PMID- 32559408
OWN - NLM
STAT- MEDLINE
DCOM- 20210730
LR  - 20210730
IS  - 1557-8100 (Electronic)
IS  - 1536-2310 (Linking)
VI  - 24
IP  - 9
DP  - 2020 Sep
TI  - Dentistry and OMICS: Transcriptome Dynamics of an Oral Ecosystem as Measured by 
      Changes in Oral Polymorphonuclear Neutrophils in Experimental Gingivitis.
PG  - 531-540
LID - 10.1089/omi.2020.0034 [doi]
AB  - Oral health and dentistry are essential components of systems medicine, which has 
      received lesser attention in comparison to other medical fields, such as cancer 
      biology. In this context, oral polymorphonuclear neutrophils (oPMNs) play an 
      important role in the maintenance of oral health. To the best of our knowledge, 
      this is the first study to report original observations on the transcriptional 
      responses of oPMNs during experimentally induced gingivitis, by temporarily 
      refraining from regular oral care. Oral rinses were prospectively collected at 
      four different time points for oPMNs isolation from healthy volunteers: day 1 
      (start of the experimental gingivitis challenge), day 9 (during challenge), day 
      14 (end of the challenge), and day 21 (postchallenge). Transcriptome of oPMNs was 
      determined by RNA sequencing. Differentially expressed genes (DEGs) were selected 
      at p < 0.01 level, and evaluated for pathway regulation using Ingenuity Pathway 
      Analysis suite. We found four major clusters of DEGs, consisting of 256 initial 
      response DEGs (day 9 only), 221 late response DEGs (day 14 only), 53 persistent 
      responsive DEGs (consistent at day 9 and 14), and 524 DEGs showing responses only 
      in the postchallenge phase (day 21 only). Pathway analysis of the initial and 
      late response DEGs showed involvement in many immune regulatory pathways and PMN 
      function, whereas DEGs at day 21 were associated with epithelial adherence 
      signaling and other miscellaneous related signaling pathways. The results from 
      this pilot study showed that oPMNs mediate oral inflammatory processes, 
      suggesting their immunomodulatory role in oral equilibrium.
FAU - Rijkschroeff, Patrick
AU  - Rijkschroeff P
AD  - Department of Periodontology, Academic Center for Dentistry Amsterdam (ACTA), 
      University of Amsterdam (UVA) and Vrije Universiteit Amsterdam (VU), Amsterdam, 
      The Netherlands.
FAU - Schoenmaker, Ton
AU  - Schoenmaker T
AD  - Department of Periodontology, Academic Center for Dentistry Amsterdam (ACTA), 
      University of Amsterdam (UVA) and Vrije Universiteit Amsterdam (VU), Amsterdam, 
      The Netherlands.
FAU - Caspers, Martien
AU  - Caspers M
AD  - Microbiology and System Biology, TNO Earth, Environmental and Life Sciences, 
      Zeist, The Netherlands.
FAU - Verschuren, Lars
AU  - Verschuren L
AD  - Microbiology and System Biology, TNO Earth, Environmental and Life Sciences, 
      Zeist, The Netherlands.
FAU - Keijser, Bart J F
AU  - Keijser BJF
AD  - Microbiology and System Biology, TNO Earth, Environmental and Life Sciences, 
      Zeist, The Netherlands.
AD  - Department of Preventive Dentistry, Academic Center for Dentistry Amsterdam 
      (ACTA), University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, The 
      Netherlands.
AD  - Top Institute Food and Nutrition, Wageningen, The Netherlands.
FAU - Nicu, Elena A
AU  - Nicu EA
AD  - Department of Periodontology, Academic Center for Dentistry Amsterdam (ACTA), 
      University of Amsterdam (UVA) and Vrije Universiteit Amsterdam (VU), Amsterdam, 
      The Netherlands.
AD  - Opris Dent SRL, Sibiu, Romania.
FAU - Loos, Bruno G
AU  - Loos BG
AD  - Department of Periodontology, Academic Center for Dentistry Amsterdam (ACTA), 
      University of Amsterdam (UVA) and Vrije Universiteit Amsterdam (VU), Amsterdam, 
      The Netherlands.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200619
PL  - United States
TA  - OMICS
JT  - Omics : a journal of integrative biology
JID - 101131135
SB  - IM
MH  - Cell Communication
MH  - Dentistry/*methods/standards
MH  - Disease Susceptibility
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation
MH  - *Genomics/methods
MH  - Gingivitis/*etiology/metabolism/pathology
MH  - Host-Pathogen Interactions/genetics/immunology
MH  - Humans
MH  - Mouth/*microbiology
MH  - Neutrophils/*immunology/*metabolism/pathology
MH  - *Oral Hygiene
MH  - Signal Transduction
OTO - NOTNLM
OT  - dentistry
OT  - gingivitis
OT  - neutrophils
OT  - oral health
OT  - transcriptomics
EDAT- 2020/06/20 06:00
MHDA- 2021/07/31 06:00
CRDT- 2020/06/20 06:00
PHST- 2020/06/20 06:00 [pubmed]
PHST- 2021/07/31 06:00 [medline]
PHST- 2020/06/20 06:00 [entrez]
AID - 10.1089/omi.2020.0034 [doi]
PST - ppublish
SO  - OMICS. 2020 Sep;24(9):531-540. doi: 10.1089/omi.2020.0034. Epub 2020 Jun 19.