PMID- 32560036
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 2073-4360 (Electronic)
IS  - 2073-4360 (Linking)
VI  - 12
IP  - 6
DP  - 2020 Jun 16
TI  - Enhanced Catalytic Dye Decolorization by Microencapsulation of Laccase from P. 
      Sanguineus CS43 in Natural and Synthetic Polymers.
LID - 10.3390/polym12061353 [doi]
LID - 1353
AB  - Polymeric microcapsules with the fungal laccase from Pycnoporus sanguineus CS43 
      may represent an attractive avenue for the removal or degradation of dyes from 
      wastewaters. Microcapsules of alginate/chitosan (9.23 +/- 0.12 microm) and 
      poly(styrenesulfonate) (PSS) (9.25 +/- 0.35 microm) were synthesized and subsequently 
      tested for catalytic activity in the decolorization of the diazo dye Congo Red. 
      Successful encapsulation into the materials was verified via confocal microscopy 
      of labeled enzyme molecules. Laccase activity was measured as a function of time 
      and the initial reaction rates were recovered for each preparation, showing up to 
      sevenfold increase with respect to free laccase. The ability of substrates to 
      diffuse through the pores of the microcapsules was evaluated with the aid of 
      fluorescent dyes and confocal microscopy. pH and thermal stability were also 
      measured for encapsulates, showing catalytic activity for pH values as low as 4 
      and temperatures of about 80  degrees C. Scanning electron microscope (SEM) analyses 
      demonstrated the ability of PSS capsules to avoid accumulation of byproducts and, 
      therefore, superior catalytic performance. This was corroborated by the direct 
      observation of substrates diffusing in and out of the materials. Compared with 
      our PSS preparation, alginate/chitosan microcapsules studied by others degrade 
      2.6 times more dye, albeit with a 135-fold increase in units of enzyme per mg of 
      dye. Similarly, poly(vinyl) alcohol microcapsules from degrade 1.7 times more 
      dye, despite an eightfold increase in units of enzyme per mg of dye. This could 
      be potentially beneficial from the economic viewpoint as a significantly lower 
      amount of enzyme might be needed for the same decolorization level achieved with 
      similar encapsulated systems.
FAU - Lopez-Barbosa, Natalia
AU  - Lopez-Barbosa N
AUID- ORCID: 0000-0001-6599-0859
AD  - Department of Electrical and Electronic Engineering, Universidad de los Andes, 
      Cra. 1E No. 19a-40, Bogota D.C. 111711, Colombia.
AD  - Department of Biomedical Engineering, Universidad de Los Andes, Cra. 1E No. 
      19a-40, Bogota D.C. 111711, Colombia.
FAU - Campana, Ana Lucia
AU  - Campana AL
AUID- ORCID: 0000-0001-8374-4942
AD  - Department of Electrical and Electronic Engineering, Universidad de los Andes, 
      Cra. 1E No. 19a-40, Bogota D.C. 111711, Colombia.
FAU - Cruz, Juan C
AU  - Cruz JC
AUID- ORCID: 0000-0002-7790-7546
AD  - Department of Biomedical Engineering, Universidad de Los Andes, Cra. 1E No. 
      19a-40, Bogota D.C. 111711, Colombia.
FAU - Ornelas-Soto, Nancy
AU  - Ornelas-Soto N
AD  - Laboratorio de Nanotecnologia Ambiental, Escuela de Ingenieria y Ciencias, 
      Tecnologico de Monterrey, Monterrey 64849, Mexico.
FAU - Osma, Johann F
AU  - Osma JF
AUID- ORCID: 0000-0003-2928-3406
AD  - Department of Electrical and Electronic Engineering, Universidad de los Andes, 
      Cra. 1E No. 19a-40, Bogota D.C. 111711, Colombia.
LA  - eng
PT  - Journal Article
DEP - 20200616
PL  - Switzerland
TA  - Polymers (Basel)
JT  - Polymers
JID - 101545357
PMC - PMC7362170
OTO - NOTNLM
OT  - P. Sanguineus CS43
OT  - azo dyes
OT  - decolorization
OT  - laccase
OT  - microcapsules
COIS- The authors declare no conflict of interest.
EDAT- 2020/06/21 06:00
MHDA- 2020/06/21 06:01
PMCR- 2020/06/16
CRDT- 2020/06/21 06:00
PHST- 2020/05/14 00:00 [received]
PHST- 2020/05/27 00:00 [revised]
PHST- 2020/05/29 00:00 [accepted]
PHST- 2020/06/21 06:00 [entrez]
PHST- 2020/06/21 06:00 [pubmed]
PHST- 2020/06/21 06:01 [medline]
PHST- 2020/06/16 00:00 [pmc-release]
AID - polym12061353 [pii]
AID - polymers-12-01353 [pii]
AID - 10.3390/polym12061353 [doi]
PST - epublish
SO  - Polymers (Basel). 2020 Jun 16;12(6):1353. doi: 10.3390/polym12061353.