PMID- 32560179
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 2077-0383 (Print)
IS  - 2077-0383 (Electronic)
IS  - 2077-0383 (Linking)
VI  - 9
IP  - 6
DP  - 2020 Jun 16
TI  - Transient and Persistent Gastric Microbiome: Adherence of Bacteria in Gastric 
      Cancer and Dyspeptic Patient Biopsies after Washing.
LID - 10.3390/jcm9061882 [doi]
LID - 1882
AB  - Helicobacter pylori is a common colonizer of the human stomach, and long-term 
      colonization has been related to development of atrophic gastritis, peptic ulcers 
      and gastric cancer. The increased gastric pH caused by H. pylori colonization, 
      treatment with antibiotics or proton pump inhibitors (PPI) may allow growth of 
      other bacteria. Previous studies have detected non-Helicobacter bacteria in 
      stomach biopsies, but no conclusion has been made of whether these represent a 
      transient contamination or a persistent microbiota. The aim of this study was to 
      evaluate the transient and persistent bacterial communities of gastric biopsies. 
      The washed or unwashed gastric biopsies were investigated by cultivation and 
      microbiota analysis (16S rRNA gene-targeted amplicon sequencing) for the 
      distribution of H. pylori and other non-Helicobacter bacteria. The number of 
      cultured non-Helicobacter bacteria decreased in the washed biopsies, suggesting 
      that they might be a transient contamination. No significant differences in the 
      bacterial diversity were observed in the microbiome analysis between unwashed and 
      washed biopsies. However, the bacterial diversity in biopsies shown H. 
      pylori-positive and H. pylori-negative were significantly different, implying 
      that H. pylori is the major modulator of the gastric microbiome. Further 
      large-scale studies are required to investigate the transient and persistent 
      gastric microbiota.
FAU - Spiegelhauer, Malene R
AU  - Spiegelhauer MR
AD  - Department of Clinical Microbiology, Rigshospitalet, Henrik Harpestrengs Vej 4A, 
      2100 Copenhagen, Denmark.
FAU - Kupcinskas, Juozas
AU  - Kupcinskas J
AUID- ORCID: 0000-0002-8760-7416
AD  - Department of Gastroenterology, Lithuanian University of Health Sciences, Eiveniu 
      str. 2, LT-50009 Kaunas, Lithuania.
AD  - Institute for Digestive Research, Lithuanian University of Health Sciences, 
      Eiveniu str. 2, LT-50009 Kaunas, Lithuania.
FAU - Johannesen, Thor B
AU  - Johannesen TB
AD  - Department of Clinical Microbiology and Infection Control, Statens Serum 
      Institute, Artillerivej 5, 2300 Copenhagen, Denmark.
FAU - Urba, Mindaugas
AU  - Urba M
AD  - Department of Gastroenterology, Lithuanian University of Health Sciences, Eiveniu 
      str. 2, LT-50009 Kaunas, Lithuania.
AD  - Institute for Digestive Research, Lithuanian University of Health Sciences, 
      Eiveniu str. 2, LT-50009 Kaunas, Lithuania.
FAU - Skieceviciene, Jurgita
AU  - Skieceviciene J
AD  - Institute for Digestive Research, Lithuanian University of Health Sciences, 
      Eiveniu str. 2, LT-50009 Kaunas, Lithuania.
FAU - Jonaitis, Laimas
AU  - Jonaitis L
AD  - Department of Gastroenterology, Lithuanian University of Health Sciences, Eiveniu 
      str. 2, LT-50009 Kaunas, Lithuania.
FAU - Frandsen, Tove H
AU  - Frandsen TH
AD  - Department of Clinical Microbiology, Rigshospitalet, Henrik Harpestrengs Vej 4A, 
      2100 Copenhagen, Denmark.
FAU - Kupcinskas, Limas
AU  - Kupcinskas L
AD  - Department of Gastroenterology, Lithuanian University of Health Sciences, Eiveniu 
      str. 2, LT-50009 Kaunas, Lithuania.
AD  - Institute for Digestive Research, Lithuanian University of Health Sciences, 
      Eiveniu str. 2, LT-50009 Kaunas, Lithuania.
FAU - Fuursted, Kurt
AU  - Fuursted K
AD  - Department of Clinical Microbiology and Infection Control, Statens Serum 
      Institute, Artillerivej 5, 2300 Copenhagen, Denmark.
FAU - Andersen, Leif P
AU  - Andersen LP
AD  - Department of Clinical Microbiology, Rigshospitalet, Henrik Harpestrengs Vej 4A, 
      2100 Copenhagen, Denmark.
LA  - eng
GR  - APP-2/2016/Lietuvos Mokslo Taryba/
PT  - Journal Article
DEP - 20200616
PL  - Switzerland
TA  - J Clin Med
JT  - Journal of clinical medicine
JID - 101606588
PMC - PMC7357088
OTO - NOTNLM
OT  - Helicobacter pylori
OT  - culture
OT  - gastric microbiota
OT  - microbiome
OT  - persistent
OT  - sequencing
OT  - transient
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study; in the collection, analyses or interpretation of data; in 
      the writing of the manuscript or in the decision to publish the results.
EDAT- 2020/06/21 06:00
MHDA- 2020/06/21 06:01
PMCR- 2020/06/16
CRDT- 2020/06/21 06:00
PHST- 2020/04/21 00:00 [received]
PHST- 2020/06/12 00:00 [revised]
PHST- 2020/06/13 00:00 [accepted]
PHST- 2020/06/21 06:00 [entrez]
PHST- 2020/06/21 06:00 [pubmed]
PHST- 2020/06/21 06:01 [medline]
PHST- 2020/06/16 00:00 [pmc-release]
AID - jcm9061882 [pii]
AID - jcm-09-01882 [pii]
AID - 10.3390/jcm9061882 [doi]
PST - epublish
SO  - J Clin Med. 2020 Jun 16;9(6):1882. doi: 10.3390/jcm9061882.