PMID- 32562559
OWN - NLM
STAT- MEDLINE
DCOM- 20210806
LR  - 20210806
IS  - 1097-0231 (Electronic)
IS  - 0951-4198 (Linking)
VI  - 34
IP  - 19
DP  - 2020 Oct 15
TI  - Pathway attenuation of fatty acid beta-oxidation in the skeletal muscle of a type 
      2 diabetic mouse model.
PG  - e8869
LID - 10.1002/rcm.8869 [doi]
AB  - RATIONALE: Whether catabolic abnormalities of fatty acids exist in the skeletal 
      muscle of type 2 diabetes mellitus (T2DM) has not been determined. In this study, 
      we postulated that a systematic evaluation of the protein abundance and metabolic 
      activity related to fatty acids in the skeletal muscle tissues of a T2DM mouse 
      model was feasible to address this question. METHODS: Mitochondria were extracted 
      from wild-type (WT) and db/db mice followed by quantitative analysis of the 
      proteins involved in mitochondrial fatty acid oxidation (mFAO). The pathway 
      activity of mFAO in skeletal muscle tissues was monitored in vitro using mass 
      spectrometry, and tissue lipidomic analysis was conducted in profiling and target 
      mode to distinguish the levels of long-chain acylcarnitines between WT and db/db 
      mice. RESULTS: Two proteins related to the mFAO pathway were significantly 
      downregulated in the skeletal muscle mitochondria of db/db mice. The measurement 
      of mFAO pathway activity in vitro revealed that the abundance of long-chain 
      acylcarnitines (C14 to C18) in db/db mice was lower than that in WT mice, and the 
      determination of acylcarnitines in skeletal muscle tissues in vivo revealed that 
      most long-chain acylcarnitines were decreased in db/db mice. CONCLUSIONS: The 
      findings of lower abundance of ACAD9 and CPT1B, reduced activity of the mFAO 
      pathway in vitro and decreased acylcarnitines in vivo firmly support that the 
      mFAO pathway in the skeletal muscle of diabetic mice is attenuated, possibly 
      resulting in cell/tissue dysfunction in diabetes.
CI  - (c) 2020 John Wiley & Sons, Ltd.
FAU - Zhou, Yang
AU  - Zhou Y
AD  - BGI-Shenzhen, Shenzhen, China.
AD  - China National GeneBank, BGI-Shenzhen, Shenzhen, China.
FAU - Tan, Yifan
AU  - Tan Y
AD  - BGI-Shenzhen, Shenzhen, China.
AD  - China National GeneBank, BGI-Shenzhen, Shenzhen, China.
FAU - Hou, Guixue
AU  - Hou G
AD  - BGI-Shenzhen, Shenzhen, China.
AD  - China National GeneBank, BGI-Shenzhen, Shenzhen, China.
FAU - Ren, Yan
AU  - Ren Y
AD  - BGI-Shenzhen, Shenzhen, China.
AD  - China National GeneBank, BGI-Shenzhen, Shenzhen, China.
FAU - Deng, Yamei
AU  - Deng Y
AD  - BGI-Shenzhen, Shenzhen, China.
AD  - China National GeneBank, BGI-Shenzhen, Shenzhen, China.
FAU - Yan, Keqiang
AU  - Yan K
AD  - BGI-Shenzhen, Shenzhen, China.
AD  - China National GeneBank, BGI-Shenzhen, Shenzhen, China.
FAU - Zhang, Yue
AU  - Zhang Y
AD  - BGI-Shenzhen, Shenzhen, China.
AD  - China National GeneBank, BGI-Shenzhen, Shenzhen, China.
FAU - Lin, Liang
AU  - Lin L
AD  - BGI-Shenzhen, Shenzhen, China.
AD  - China National GeneBank, BGI-Shenzhen, Shenzhen, China.
FAU - Lou, Xiaomin
AU  - Lou X
AD  - Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China.
FAU - Liu, Siqi
AU  - Liu S
AUID- ORCID: 0000-0001-9744-3681
AD  - BGI-Shenzhen, Shenzhen, China.
AD  - China National GeneBank, BGI-Shenzhen, Shenzhen, China.
LA  - eng
GR  - 31700728/National Natural Science Foundation of China/
GR  - DRC-SZ[2016]749/Shenzhen Engineering Laboratory for Proteomics/
PT  - Journal Article
PL  - England
TA  - Rapid Commun Mass Spectrom
JT  - Rapid communications in mass spectrometry : RCM
JID - 8802365
RN  - 0 (Fatty Acids)
SB  - IM
MH  - Animals
MH  - Diabetes Mellitus, Experimental
MH  - Diabetes Mellitus, Type 2/*metabolism
MH  - *Fatty Acids/chemistry/metabolism
MH  - Lipidomics
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mitochondria, Muscle/*metabolism
MH  - Muscle, Skeletal/*cytology
MH  - Oxidation-Reduction
MH  - Tandem Mass Spectrometry
EDAT- 2020/06/21 06:00
MHDA- 2021/08/07 06:00
CRDT- 2020/06/21 06:00
PHST- 2020/02/17 00:00 [received]
PHST- 2020/05/21 00:00 [revised]
PHST- 2020/06/09 00:00 [accepted]
PHST- 2020/06/21 06:00 [pubmed]
PHST- 2021/08/07 06:00 [medline]
PHST- 2020/06/21 06:00 [entrez]
AID - 10.1002/rcm.8869 [doi]
PST - ppublish
SO  - Rapid Commun Mass Spectrom. 2020 Oct 15;34(19):e8869. doi: 10.1002/rcm.8869.