PMID- 32564013
OWN - NLM
STAT- MEDLINE
DCOM- 20210906
LR  - 20210906
IS  - 1423-0259 (Electronic)
IS  - 0030-3747 (Linking)
VI  - 64
IP  - 1
DP  - 2021
TI  - A Comparison of Subconjunctival Wound Healing between Different Methods of 
      Dissecting Subconjunctival Tissues.
PG  - 99-107
LID - 10.1159/000509551 [doi]
AB  - OBJECTIVE: To compare different methods for dissecting subconjunctival tissues by 
      developing subconjunctival wound healing models. METHODS: New Zealand white 
      rabbits were separated into 3 groups based on the method by which the rabbit 
      subconjunctival wound healing model was generated: subconjunctival tissues were 
      dissected episclerally (EPI) or subepithelially (SUB), with a corresponding blank 
      control (CON). All the cases in the experimental groups were surgically prepared 
      with conjunctival flaps, and they were sacrificed on the third postoperative day. 
      At the surgical sites, the protein levels of hypoxia-inducible factor-1 
      (HIF-1)-alpha, vascular endothelial growth factor (VEGF)-A, and matrix 
      metalloproteinase (MMP)-2 were detected by Western blot, morphological 
      vascularity was measured by Adobe Photoshop, and subconjunctival fibrosis was 
      assessed by histology. RESULTS: Compared with the CON group, both the EPI and SUB 
      groups showed significantly upregulated protein levels of HIF-1alpha, VEGF-A, and 
      MMP-2. In addition, the protein levels of HIF-1alpha, VEGF-A, and MMP-2 were higher 
      in the EPI group than in the SUB group. Morphological vascularity was 
      significantly elevated in the EPI group compared with the SUB and CON groups. 
      Collagen content was markedly increased in the EPI group compared with the SUB 
      and CON groups. CONCLUSIONS: Dissecting subconjunctival tissues subepithelially 
      inhibits subconjunctival fibrosis, which may be instructive in tenonectomy in 
      filtration surgery.
CI  - (c) 2020 S. Karger AG, Basel.
FAU - Gong, Ruowen
AU  - Gong R
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen 
      University, Guangzhou, China.
AD  - Department of Ophthalmology, Eye and ENT Hospital of Fudan University, Shanghai, 
      China.
FAU - Zuo, Chengguo
AU  - Zuo C
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen 
      University, Guangzhou, China.
FAU - Wu, Keling
AU  - Wu K
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen 
      University, Guangzhou, China.
FAU - Zhang, Simin
AU  - Zhang S
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen 
      University, Guangzhou, China.
FAU - Qin, Xi
AU  - Qin X
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen 
      University, Guangzhou, China.
FAU - Li, Yangyang
AU  - Li Y
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen 
      University, Guangzhou, China.
FAU - Gao, Xinbo
AU  - Gao X
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen 
      University, Guangzhou, China.
FAU - Huang, Danping
AU  - Huang D
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen 
      University, Guangzhou, China.
FAU - Lin, Mingkai
AU  - Lin M
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen 
      University, Guangzhou, China, linmk@mail.sysu.edu.cn.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20200619
PL  - Switzerland
TA  - Ophthalmic Res
JT  - Ophthalmic research
JID - 0267442
SB  - IM
MH  - Animals
MH  - Conjunctiva/*surgery
MH  - Disease Models, Animal
MH  - Female
MH  - Filtering Surgery/*methods
MH  - Glaucoma/*surgery
MH  - Rabbits
MH  - *Wound Healing
OTO - NOTNLM
OT  - Conjunctival flap
OT  - Glaucoma filtration surgery
OT  - Tenonectomy
OT  - Wound healing
EDAT- 2020/06/22 06:00
MHDA- 2021/09/07 06:00
CRDT- 2020/06/22 06:00
PHST- 2020/03/14 00:00 [received]
PHST- 2020/06/17 00:00 [accepted]
PHST- 2020/06/22 06:00 [pubmed]
PHST- 2021/09/07 06:00 [medline]
PHST- 2020/06/22 06:00 [entrez]
AID - 000509551 [pii]
AID - 10.1159/000509551 [doi]
PST - ppublish
SO  - Ophthalmic Res. 2021;64(1):99-107. doi: 10.1159/000509551. Epub 2020 Jun 19.