PMID- 32566004
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 20
IP  - 1
DP  - 2020 Jul
TI  - Long non-coding RNA AC023794.4-201 exerts a tumor-suppressive function in 
      laryngeal squamous cell cancer and may serve as a potential prognostic biomarker.
PG  - 774-784
LID - 10.3892/ol.2020.11595 [doi]
AB  - After the expression level of lncRNA AC023794.4-201 was upregulated in 2 
      laryngeal squamous cell carcinoma (LSCC) cell lines (AMC-HN-8 and TU-212) and 
      LSCC xenografts, the biological function of lncRNA AC023794.4-201 in LSCC was 
      further investigated using in vitro and in vivo experiments, such as cell 
      function experiments and nude mice transplantation. In our previous study, it was 
      demonstrated that the expression level of the long non-coding RNA (lncRNA) 
      AC023794.4-201 were decreased in laryngeal squamous cell carcinoma, particularly 
      in cases of LSCC with lymphatic metastasis. Moreover, low expression levels of 
      AC023794.4-201 were revealed to be an adverse prognostic factor for patients with 
      LSCC. In the present study, lentiviruses were used to overexpress AC023794.4-201 
      before a series of cell function assays were performed and a xenograft nude mouse 
      model was constructed, in order to further investigate the functions of 
      AC023794.4-201 in LSCC. AC023794.4-201 inhibited the proliferation and the 
      cloning capacity of LSCC cells compared with the negative control group as 
      indicated by real-time cell analysis and the plate colony formation assay. Flow 
      cytometry and transwell migration assays demonstrated that AC023794.4-201 
      inhibited the migration, induced cell cycle arrest and increased the apoptotic 
      rate of LSCC cells. The results of the in vivo studies demonstrated that 
      AC023794.4-201 significantly inhibited the growth of LSCC xenografts, and 
      promoted apoptosis. In conclusion, the findings of the present study suggested 
      that AC023794.4-201 may exert tumor-suppressive functions in the progression of 
      LSCC and may serve as a potential prognostic biomarker for LSCC.
CI  - Copyright: (c) Shen et al.
FAU - Shen, Zhisen
AU  - Shen Z
AD  - Department of Otorhinolaryngology and Head and Neck Surgery, Li Huili Hospital 
      Affiliated to Ningbo University, Ningbo, Zhejiang 315040, P.R. China.
FAU - Yuan, Jie
AU  - Yuan J
AD  - Department of Otorhinolaryngology and Head and Neck Surgery, Li Huili Hospital 
      Affiliated to Ningbo University, Ningbo, Zhejiang 315040, P.R. China.
AD  - Department of Otorhinolaryngology, Medical School of Ningbo University, Ningbo, 
      Zhejiang 315211, P.R. China.
FAU - Tong, Qiaoling
AU  - Tong Q
AD  - Department of Otorhinolaryngology and Head and Neck Surgery, Ningbo No. 2 
      Hospital, Ningbo, Zhejiang 315010, P.R. China.
FAU - Hao, Wenjuan
AU  - Hao W
AD  - Department of Otorhinolaryngology and Head and Neck Surgery, Li Huili Hospital 
      Affiliated to Ningbo University, Ningbo, Zhejiang 315040, P.R. China.
FAU - Deng, Hongxia
AU  - Deng H
AD  - Department of Otorhinolaryngology and Head and Neck Surgery, Li Huili Hospital 
      Affiliated to Ningbo University, Ningbo, Zhejiang 315040, P.R. China.
FAU - Li, Qun
AU  - Li Q
AD  - Department of Otorhinolaryngology and Head and Neck Surgery, Li Huili Hospital 
      Affiliated to Ningbo University, Ningbo, Zhejiang 315040, P.R. China.
FAU - Zhou, Chongchang
AU  - Zhou C
AD  - Department of Otorhinolaryngology and Head and Neck Surgery, Li Huili Hospital 
      Affiliated to Ningbo University, Ningbo, Zhejiang 315040, P.R. China.
FAU - Hu, Yan
AU  - Hu Y
AD  - Department of Otorhinolaryngology and Head and Neck Surgery, Li Huili Hospital 
      Affiliated to Ningbo University, Ningbo, Zhejiang 315040, P.R. China.
AD  - Department of Otorhinolaryngology, Medical School of Ningbo University, Ningbo, 
      Zhejiang 315211, P.R. China.
FAU - Xu, Jie
AU  - Xu J
AD  - Department of Otorhinolaryngology and Head and Neck Surgery, Li Huili Hospital 
      Affiliated to Ningbo University, Ningbo, Zhejiang 315040, P.R. China.
AD  - Department of Otorhinolaryngology, Medical School of Ningbo University, Ningbo, 
      Zhejiang 315211, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20200507
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC7286120
OTO - NOTNLM
OT  - AC023794.4-201
OT  - laryngeal squamous cell carcinoma
OT  - long non-coding RNA
OT  - prognostic biomarker
EDAT- 2020/06/23 06:00
MHDA- 2020/06/23 06:01
PMCR- 2020/05/07
CRDT- 2020/06/23 06:00
PHST- 2019/07/28 00:00 [received]
PHST- 2020/02/20 00:00 [accepted]
PHST- 2020/06/23 06:00 [entrez]
PHST- 2020/06/23 06:00 [pubmed]
PHST- 2020/06/23 06:01 [medline]
PHST- 2020/05/07 00:00 [pmc-release]
AID - OL-0-0-11595 [pii]
AID - 10.3892/ol.2020.11595 [doi]
PST - ppublish
SO  - Oncol Lett. 2020 Jul;20(1):774-784. doi: 10.3892/ol.2020.11595. Epub 2020 May 7.