PMID- 32566480
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240330
IS  - 2213-5979 (Print)
IS  - 2213-5979 (Electronic)
IS  - 2213-5979 (Linking)
VI  - 20
DP  - 2020 Dec
TI  - Simultaneous photoacoustic microscopy, spectral-domain optical coherence 
      tomography, and fluorescein microscopy multi-modality retinal imaging.
PG  - 100194
LID - 10.1016/j.pacs.2020.100194 [doi]
LID - 100194
AB  - The goal of this study is to further develop a multi-modality eye imaging system 
      and evaluate its feasibility of acquiring images of different modalities 
      simultaneously. An integrated multimodality imaging system combining 
      spectral-domain optical coherence tomography (SD-OCT), photoacoustic microscopy 
      (PAM), and fluorescence microscopy (FM) was developed, and its performance for 
      eye imaging was validated on multiple clinically-relevant retinal disease models 
      in vivo in rabbits. OCT imaging allows for visualization of the different 
      anatomic retinal layers with high axial resolution. PAM can be used to image 
      vasculature, angiogenesis, and hemorrhages. The leakage of neovascularization can 
      be verified with FM and fluorescein dye. Simultaneous imaging with OCT, PAM, and 
      FM ensures co-registration of the three modalities without being affected by 
      motion artifacts caused by breathing, body or eye movements, and heartbeat. This 
      simultaneous multi-modality eye imaging system could be a new tool for 
      applications both in ophthalmology and other fields.
CI  - (c) 2020 The Authors.
FAU - Zhang, Wei
AU  - Zhang W
AD  - Department of Biomedical Engineering, University of Michigan, Ann Arbor, United 
      States.
FAU - Li, Yanxiu
AU  - Li Y
AD  - Department of Ophthalmology and Visual Sciences, University of Michigan, Ann 
      Arbor, United States.
AD  - Department of Ophthalmology, Xiangya Hospital, Central South University, 
      Changsha, PR China.
FAU - Yu, Yixin
AU  - Yu Y
AD  - Department of Ophthalmology and Visual Sciences, University of Michigan, Ann 
      Arbor, United States.
AD  - Department of Ophthalmology, Xiangya Hospital, Central South University, 
      Changsha, PR China.
FAU - Derouin, Katherine
AU  - Derouin K
AD  - Department of Ophthalmology and Visual Sciences, University of Michigan, Ann 
      Arbor, United States.
FAU - Qin, Yu
AU  - Qin Y
AD  - Department of Biomedical Engineering, University of Michigan, Ann Arbor, United 
      States.
FAU - Nguyen, V Phuc
AU  - Nguyen VP
AD  - Department of Ophthalmology and Visual Sciences, University of Michigan, Ann 
      Arbor, United States.
FAU - Xia, Xiaobo
AU  - Xia X
AD  - Department of Ophthalmology, Xiangya Hospital, Central South University, 
      Changsha, PR China.
FAU - Wang, Xueding
AU  - Wang X
AD  - Department of Biomedical Engineering, University of Michigan, Ann Arbor, United 
      States.
FAU - Paulus, Yannis M
AU  - Paulus YM
AD  - Department of Biomedical Engineering, University of Michigan, Ann Arbor, United 
      States.
AD  - Department of Ophthalmology and Visual Sciences, University of Michigan, Ann 
      Arbor, United States.
LA  - eng
GR  - K08 EY027458/EY/NEI NIH HHS/United States
GR  - P30 EY007003/EY/NEI NIH HHS/United States
GR  - R01 EY029489/EY/NEI NIH HHS/United States
PT  - Journal Article
DEP - 20200606
PL  - Germany
TA  - Photoacoustics
JT  - Photoacoustics
JID - 101622604
PMC - PMC7298671
OTO - NOTNLM
OT  - Fluorescence microscopy
OT  - Multi-modality imaging
OT  - Optical coherence tomography
OT  - Photoacoustic microscopy
OT  - Simultaneous imaging
EDAT- 2020/06/23 06:00
MHDA- 2020/06/23 06:01
PMCR- 2020/06/06
CRDT- 2020/06/23 06:00
PHST- 2020/01/20 00:00 [received]
PHST- 2020/05/28 00:00 [revised]
PHST- 2020/06/02 00:00 [accepted]
PHST- 2020/06/23 06:00 [entrez]
PHST- 2020/06/23 06:00 [pubmed]
PHST- 2020/06/23 06:01 [medline]
PHST- 2020/06/06 00:00 [pmc-release]
AID - S2213-5979(20)30034-3 [pii]
AID - 100194 [pii]
AID - 10.1016/j.pacs.2020.100194 [doi]
PST - epublish
SO  - Photoacoustics. 2020 Jun 6;20:100194. doi: 10.1016/j.pacs.2020.100194. 
      eCollection 2020 Dec.