PMID- 32567002
OWN - NLM
STAT- MEDLINE
DCOM- 20211126
LR  - 20211204
IS  - 1869-1889 (Electronic)
IS  - 1674-7305 (Linking)
VI  - 64
IP  - 1
DP  - 2021 Jan
TI  - FoxO directly regulates the expression of TOR/S6K and vitellogenin to modulate 
      the fecundity of the brown planthopper.
PG  - 133-143
LID - 10.1007/s11427-019-1734-6 [doi]
AB  - As a conserved transcription factor, FoxO plays a crucial role in multiple 
      physiological processes in vivo, including stress resistance, longevity, growth 
      and reproduction. Previous studies on FoxO have focused on human, mouse, 
      Drosophila melanogaster and Caenorhabditis elegans, while there are few reports 
      on agricultural pests and little is known about how FoxO modulates insect 
      fecundity. In Asia, the brown planthopper (BPH) Nilaparvata lugens (Stal) is one 
      of the most serious pests in rice production and high fecundity is the basis of 
      the outbreak of BPH. Here, using the genome-wide ChIP-seq of NlFoxO in BPH, we 
      found that NlFoxO binds to the promoters of ribosomal proteinS6 kinase (NlS6K) 
      and serine/threonine-protein kinase mTOR (NlTOR) and increases their expression 
      levels. We also found that NlFoxO directly binds to the exon of vitellogenin 
      (NlVg) and has a specific inhibitory effect on its expression. In addition, the 
      number of eggs laid and their hatching rate decreased significantly after 
      injection of NlFoxO double-stranded RNA into BPH adults. Our findings provide 
      direct evidence that FoxO modulates insect fecundity through binding to the 
      promoters of NlS6K, NlTOR and the exon of NlVg and affecting their gene 
      expression in the Vg network.
FAU - Dong, Yi
AU  - Dong Y
AD  - State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen 
      University, Guangzhou, 510275, China.
FAU - Chen, Weiwen
AU  - Chen W
AD  - State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen 
      University, Guangzhou, 510275, China.
FAU - Kang, Kui
AU  - Kang K
AD  - State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen 
      University, Guangzhou, 510275, China.
FAU - Pang, Rui
AU  - Pang R
AD  - State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen 
      University, Guangzhou, 510275, China.
FAU - Dong, Yipei
AU  - Dong Y
AD  - State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen 
      University, Guangzhou, 510275, China.
FAU - Liu, Kai
AU  - Liu K
AD  - State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen 
      University, Guangzhou, 510275, China.
FAU - Zhang, Wenqing
AU  - Zhang W
AD  - State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen 
      University, Guangzhou, 510275, China. lsszwq@mail.sysu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20200618
PL  - China
TA  - Sci China Life Sci
JT  - Science China. Life sciences
JID - 101529880
RN  - 0 (Forkhead Transcription Factors)
RN  - 0 (Insect Proteins)
RN  - 0 (Vitellogenins)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Binding Sites/genetics
MH  - Blotting, Western
MH  - Chromatin Immunoprecipitation Sequencing/methods
MH  - Female
MH  - Fertility/genetics
MH  - Forkhead Transcription Factors/*genetics/metabolism
MH  - *Gene Expression Regulation
MH  - Hemiptera/*genetics/metabolism/physiology
MH  - Insect Proteins/*genetics/metabolism
MH  - Oryza/parasitology
MH  - Protein Binding
MH  - RNA Interference
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Ribosomal Protein S6 Kinases/*genetics/metabolism
MH  - TOR Serine-Threonine Kinases/*genetics/metabolism
MH  - Vitellogenins/*genetics/metabolism
OTO - NOTNLM
OT  - ChIP-seq
OT  - FoxO
OT  - Nilaparvata lugens
OT  - bind
OT  - fecundity
OT  - vitellogenin (Vg)
EDAT- 2020/06/23 06:00
MHDA- 2021/11/27 06:00
CRDT- 2020/06/23 06:00
PHST- 2019/12/25 00:00 [received]
PHST- 2020/04/29 00:00 [accepted]
PHST- 2020/06/23 06:00 [pubmed]
PHST- 2021/11/27 06:00 [medline]
PHST- 2020/06/23 06:00 [entrez]
AID - 10.1007/s11427-019-1734-6 [pii]
AID - 10.1007/s11427-019-1734-6 [doi]
PST - ppublish
SO  - Sci China Life Sci. 2021 Jan;64(1):133-143. doi: 10.1007/s11427-019-1734-6. Epub 
      2020 Jun 18.