PMID- 32568883
OWN - NLM
STAT- MEDLINE
DCOM- 20210413
LR  - 20221005
IS  - 1533-404X (Electronic)
IS  - 0195-7910 (Print)
IS  - 0195-7910 (Linking)
VI  - 41
IP  - 4
DP  - 2020 Dec
TI  - Reliable Postmortem Molecular Diagnosis of Anaphylaxis: Co-localization of Mast 
      Cell Degranulation and Immunoglobulin E in Allergic Throat Tissues.
PG  - 249-258
LID - 10.1097/PAF.0000000000000572 [doi]
AB  - Anaphylaxis is a serious reaction that may cause death in half an hour without 
      diagnostic characteristic in autopsies. Mast cell (MC) degranulation combined 
      with immunoglobulin E (IgE) plays the key roles in anaphylaxis. Unavailability of 
      serum and instability of measured serum in postmortem diagnoses sometimes limit 
      the opinion of medical experts. Allergic tissues are more accessible than serum, 
      and there is a little research on degranulated mast cells and IgE in different 
      human tissues, whereas we hardly know whether the expression will keep stable 
      over the increasing postmortem interval (PMI). In this research, we examined the 
      mast cell counts and degranulation rates and gE contents in human throat, lung, 
      and intestine tissues and preliminarily investigated the correlation of these 
      markers with PMI in anaphylaxis-associated death. Allergic samples showed a 
      significant increase in mast cell degranulation accompanied by an increase in IgE 
      levels than the control group, but the expression was not significantly 
      correlated with increasing PMI only in throat tissues. Elevated mast cell 
      degranulation combined with increased IgE levels may be a reliable biomarker for 
      forensic diagnosis of human tissues due to IgE-mediated allergic sudden death.
FAU - Wang, Xiaoyan
AU  - Wang X
AD  - From the Department of General Pathology, The First Affiliated Hospital, School 
      of Medicine, Yangtze University, Jingzhou, Hubei Province.
FAU - Yin, Chuanchang
AU  - Yin C
AD  - From the Department of General Pathology, The First Affiliated Hospital, School 
      of Medicine, Yangtze University, Jingzhou, Hubei Province.
FAU - Su, Xue
AU  - Su X
AD  - Institute of Clinical Pathology, Medical College of Shantou University, Shantou, 
      Guangdong Province, China.
FAU - Su, Min
AU  - Su M
AD  - Institute of Clinical Pathology, Medical College of Shantou University, Shantou, 
      Guangdong Province, China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Forensic Med Pathol
JT  - The American journal of forensic medicine and pathology
JID - 8108948
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Anaphylaxis/*pathology
MH  - Case-Control Studies
MH  - *Cell Degranulation
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - Humans
MH  - Immunoglobulin E/*metabolism
MH  - Immunohistochemistry
MH  - Infant
MH  - Infant, Newborn
MH  - Intestinal Mucosa/metabolism/pathology
MH  - Larynx/metabolism/pathology
MH  - Lung/pathology
MH  - Male
MH  - Mast Cells/*pathology
MH  - Middle Aged
MH  - Pharynx/metabolism/*pathology
MH  - *Postmortem Changes
MH  - Young Adult
PMC - PMC7668346
COIS- The authors report no conflict of interest.
EDAT- 2020/06/23 06:00
MHDA- 2021/04/14 06:00
PMCR- 2020/11/16
CRDT- 2020/06/23 06:00
PHST- 2020/06/23 06:00 [pubmed]
PHST- 2021/04/14 06:00 [medline]
PHST- 2020/06/23 06:00 [entrez]
PHST- 2020/11/16 00:00 [pmc-release]
AID - 00000433-202012000-00003 [pii]
AID - PAF50427 [pii]
AID - 10.1097/PAF.0000000000000572 [doi]
PST - ppublish
SO  - Am J Forensic Med Pathol. 2020 Dec;41(4):249-258. doi: 
      10.1097/PAF.0000000000000572.