PMID- 32569193
OWN - NLM
STAT- MEDLINE
DCOM- 20200706
LR  - 20221005
IS  - 1536-5964 (Electronic)
IS  - 0025-7974 (Print)
IS  - 0025-7974 (Linking)
VI  - 99
IP  - 25
DP  - 2020 Jun 19
TI  - Different effects of total flavonoids from Arachniodes exilis on human umbilical 
      cord mesenchymal stem cells in vitro.
PG  - e20628
LID - 10.1097/MD.0000000000020628 [doi]
LID - e20628
AB  - Traditional Chinese medicines are used in promotion of fractured bone healing and 
      bone diseases. Some studies reported total flavonoids from plant can be used as 
      an auxiliary source of exogenous.Use different methods to identify and verify 
      effects of total flavonoids from Arachniodes exilis (TFAE) on human umbilical 
      cord mesenchymal stem cells (HUCMSCs) in vitro.Concentrations of 1 and 5 mug/mL 
      TFAE significantly increased ALPase activity in HUCMSCs compared to the other 
      concentrations at days 3 and 7 (P < .05). RT-PCR showed that expression levels of 
      osteogenic genes (Col1a1, OPN, Runx2 and Osx) were remarkably enhanced in HUCMSCs 
      following treatment with different concentrations of TFAE for 9 days compared 
      with 0 mug/mL TFAE group (control). The results showed that 
      concentration < 5 mug/mL of TFAE induced osteogenic differentiation in HUCMSCs 
      Alizarin red staining assays revealed that both TFAE and S1191 was significantly 
      decreased (7.80 +/- 0.66) compared with the TFAE group (16.00 +/- 0.97) (P < .01). 
      ALPase activity on days 3 and 7 was relatively lower in HUCMSCs grown in media 
      supplemented with both S1191 and TFAE than that of in TFAE group only. The 
      results indicated that osteogenic markers (Col1a1, OPN, Runx2 and Osx) were 
      significantly downregulated in the TFAE + S1191 group in comparison to the 
      control group. The expressions of Col1a and OPN in the TFAE + S1191 group 
      decreased significantly (P < .01) by Western blotting.TFAE promotes the 
      odonto/osteogenic differentiation of human UCMSCs via activation of ER.
FAU - Yu, Wenmin
AU  - Yu W
AD  - The School of Basic Medical Science, Jiujiang University/Jiujiang Key Laboratory 
      of Translational Medicine, Jiujiang.
FAU - Hu, Wenlong
AU  - Hu W
AD  - Medicine Graduate School of Nanchang University.
AD  - Department of Orthopedics, The Second Hospital Affiliated to Nanchang University, 
      Nanchang, P. R. China.
FAU - Ke, Xiumei
AU  - Ke X
AUID- ORCID: 0000-0003-3829-4182
AD  - The School of Basic Medical Science, Jiujiang University/Jiujiang Key Laboratory 
      of Translational Medicine, Jiujiang.
FAU - Zhou, Xufeng
AU  - Zhou X
AD  - The School of Basic Medical Science, Jiujiang University/Jiujiang Key Laboratory 
      of Translational Medicine, Jiujiang.
FAU - Yin, Changchang
AU  - Yin C
AUID- ORCID: 0000-0001-7999-0104
AD  - The School of Basic Medical Science, Jiujiang University/Jiujiang Key Laboratory 
      of Translational Medicine, Jiujiang.
FAU - Yin, Ming
AU  - Yin M
AD  - Medicine Graduate School of Nanchang University.
AD  - Department of Orthopedics, The Second Hospital Affiliated to Nanchang University, 
      Nanchang, P. R. China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Medicine (Baltimore)
JT  - Medicine
JID - 2985248R
RN  - 0 (Flavonoids)
RN  - 0 (Plant Extracts)
SB  - IM
MH  - Cell Differentiation/drug effects
MH  - Cells, Cultured
MH  - Flavonoids/*pharmacology
MH  - Humans
MH  - In Vitro Techniques
MH  - Medicine, Chinese Traditional
MH  - Mesenchymal Stem Cells/*drug effects
MH  - Osteogenesis/*drug effects
MH  - Plant Extracts/*pharmacology
MH  - Plants, Medicinal/*chemistry
MH  - Umbilical Cord/*cytology
PMC - PMC7310876
COIS- The authors have no conflicts of interest to disclose.
EDAT- 2020/06/23 06:00
MHDA- 2020/07/07 06:00
PMCR- 2020/06/19
CRDT- 2020/06/23 06:00
PHST- 2020/06/23 06:00 [entrez]
PHST- 2020/06/23 06:00 [pubmed]
PHST- 2020/07/07 06:00 [medline]
PHST- 2020/06/19 00:00 [pmc-release]
AID - 00005792-202006190-00041 [pii]
AID - MD D-20-01165 [pii]
AID - 10.1097/MD.0000000000020628 [doi]
PST - ppublish
SO  - Medicine (Baltimore). 2020 Jun 19;99(25):e20628. doi: 
      10.1097/MD.0000000000020628.