PMID- 32572910
OWN - NLM
STAT- MEDLINE
DCOM- 20210421
LR  - 20210421
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 24
IP  - 11
DP  - 2020 Jun
TI  - LncRNA ROR promotes proliferation of endometrial cancer cells via regulating 
      Notch1 pathway.
PG  - 5970-5978
LID - 21490 [pii]
LID - 10.26355/eurrev_202006_21490 [doi]
AB  - OBJECTIVE: The aim of this study was to investigate the effects of long 
      non-coding ribonucleic acid regulator of reprogramming (lncRNA ROR) on the 
      proliferation and apoptosis of endometrial cancer (EC) cells, and to explore its 
      possible underlying mechanism. PATIENTS AND METHODS: The expression levels of 
      lncRNA ROR and Notch1 in EC tissues were detected via quantitative reverse 
      transcription-polymerase chain reaction (qRT-PCR). The changes in Notch1 protein 
      were detected via Western blotting. Subsequently, the regulatory mechanism of 
      lncRNA ROR on Notch1 was analyzed using Luciferase reporter gene assay. Moreover, 
      the changes in cell proliferation and apoptosis were determined through cell 
      counting kit-8 (CCK-8) assay and terminal deoxynucleotidyl transferase-mediated 
      dUTP nick end labeling (TUNEL) assay, respectively. RESULTS: Both lncRNA ROR and 
      Notch1 were highly expressed in EC tissues (p<0.05). After overexpression of 
      lncRNA ROR, HEC-1A cells had significantly enhanced proliferation (p<0.05) and 
      weakened apoptosis (p<0.05). Meanwhile, the mRNA and protein levels of Notch1 
      rose remarkably compared with those in control group (p<0.05). Luciferase 
      reporter gene assay revealed that lncRNA ROR could bind to the Notch1 regulatory 
      factor miR-34a and inhibit its activity. CONCLUSIONS: LncRNA ROR regulates the 
      proliferation and apoptosis of EC cells via promoting the expression of Notch1 
      protein.
FAU - Zeng, S-Y
AU  - Zeng SY
AD  - Department of Obstetrics & Gynecology, The Fifth Affiliated Hospital, Sun Yat-sen 
      University, Zhuhai, China. shisq2001@126.com.
FAU - Liu, C-Q
AU  - Liu CQ
FAU - Zhuang, Y
AU  - Zhuang Y
FAU - Chen, Y
AU  - Chen Y
FAU - Gu, L-L
AU  - Gu LL
FAU - Shi, S-Q
AU  - Shi SQ
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (MIRN34 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (NOTCH1 protein, human)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (Receptor, Notch1)
SB  - IM
MH  - Apoptosis
MH  - Cell Proliferation
MH  - Endometrial Neoplasms/*metabolism/pathology
MH  - Female
MH  - Humans
MH  - MicroRNAs/genetics/metabolism
MH  - RNA, Long Noncoding/genetics/*metabolism
MH  - Receptor, Notch1/genetics/*metabolism
MH  - Tumor Cells, Cultured
EDAT- 2020/06/24 06:00
MHDA- 2021/04/22 06:00
CRDT- 2020/06/24 06:00
PHST- 2020/06/24 06:00 [entrez]
PHST- 2020/06/24 06:00 [pubmed]
PHST- 2021/04/22 06:00 [medline]
AID - 21490 [pii]
AID - 10.26355/eurrev_202006_21490 [doi]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2020 Jun;24(11):5970-5978. doi: 
      10.26355/eurrev_202006_21490.