PMID- 32572931
OWN - NLM
STAT- MEDLINE
DCOM- 20210421
LR  - 20211204
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 24
IP  - 11
DP  - 2020 Jun
TI  - MiR-199a modulates autophagy and inflammation in rats with cerebral infarction 
      via regulating mTOR expression.
PG  - 6338-6345
LID - 21532 [pii]
LID - 10.26355/eurrev_202006_21532 [doi]
AB  - OBJECTIVE: The aim of this study was to investigate the roles of micro 
      ribonucleic acid (miR)-199a in rats with cerebral infarction by regulating 
      mammalian target of rapamycin (mTOR). MATERIALS AND METHODS: A total of 36 
      Sprague-Dawley rats were randomly assigned into three groups, including: sham 
      group (n=12), model group (n=12) and miR-199a mimics group (n=12). In sham group 
      internal and external carotid arteries were exposed. The ischemia-reperfusion 
      model was successfully established using suture embolization in the other two 
      groups. After modeling, rats in sham group and model group were intraperitoneally 
      injected with normal saline. However, rats in miR-199a mimics group were injected 
      with miR-199a mimics. Following intervention for 3 d, sampling was conducted. 
      Neurological deficit was evaluated in rats based on the Zea-Longa scoring system. 
      Hematoxylin-eosin (HE) staining was performed to observe neuronal morphology. The 
      expression of mTOR was detected using immunohistochemistry, and the relative 
      expression level of tau protein was determined via Western blotting (WB). 
      Besides, the messenger RNA (mRNA) expressions of mTOR and tau were detected by 
      quantitative Polymerase Chain Reaction (qPCR). Finally, inflammatory factor 
      content was measured through enzyme-linked immunosorbent assay (ELISA). RESULTS: 
      Model group and miR-199a mimics group exhibited a substantially higher Zea-Longa 
      score than sham group (p<0.05). Compared with model group, the Zea-Longa score 
      rose prominently in miR-199a mimics group (p<0.05). According to the results of 
      HE staining, the structure of neurons in sham group was clear and intact, while 
      the structure of neurons in model group was disordered. Meanwhile, neuronal 
      morphology in miR-199a mimics group was significantly worse than that in model 
      group (p<0.05). Immunohistochemistry results demonstrated that the positive 
      expression level of mTOR was considerably upregulated in both model group and 
      miR-199a mimics group in comparison with sham group (p<0.05). Moreover, its 
      positive expression level in miR-199a mimics group was markedly higher that in 
      model group (p<0.05). Based on the results of WB, model and miR-199a mimics 
      groups exhibited a remarkably higher relative expression level of tau protein 
      than sham group (p<0.05). However, the relative expression level of tau protein 
      in miR-199a mimics group was prominently higher than that in model group 
      (p<0.05). QPCR results manifested that the relative mRNA expression levels of 
      mTOR and tau in model group and miR-199a mimics group were dramatically higher 
      than those in sham group (p<0.05). Compared with those in model group, the 
      relative mRNA expression levels of mTOR and tau increased significantly in 
      miR-199a mimics group (p<0.05). ELISA results revealed that model group and 
      miR-199a mimics group had prominently higher content of inflammatory factors than 
      sham group (p<0.05). In addition, content of inflammatory factors in miR-199a 
      mimics group was considerably higher than that in model group (p<0.05). 
      CONCLUSIONS: MiR-199a modulates mTOR expression to exert important regulatory 
      effects on the autophagy and inflammation in rats with cerebral infarction.
FAU - Zhou, J
AU  - Zhou J
AD  - Department of Internal Medicine, Wuhan Hospital of Traditional Chinese Medicine, 
      Wuhan, China. 349033774@qq.com.
FAU - Wu, J-S
AU  - Wu JS
FAU - Yan, Y
AU  - Yan Y
FAU - Li, J
AU  - Li J
FAU - Ni, T
AU  - Ni T
FAU - Shao, W
AU  - Shao W
FAU - Mei, J-H
AU  - Mei JH
FAU - Xiong, W-Z
AU  - Xiong WZ
FAU - Wu, H
AU  - Wu H
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (MIRN199 microRNA, rat)
RN  - 0 (MicroRNAs)
RN  - EC 2.7.1.1 (mTOR protein, rat)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - *Autophagy
MH  - Cerebral Infarction/*metabolism/pathology
MH  - Inflammation/*metabolism/pathology
MH  - Injections, Intraperitoneal
MH  - MicroRNAs/administration & dosage/genetics/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - TOR Serine-Threonine Kinases/*metabolism
EDAT- 2020/06/24 06:00
MHDA- 2021/04/22 06:00
CRDT- 2020/06/24 06:00
PHST- 2020/06/24 06:00 [entrez]
PHST- 2020/06/24 06:00 [pubmed]
PHST- 2021/04/22 06:00 [medline]
AID - 21532 [pii]
AID - 10.26355/eurrev_202006_21532 [doi]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2020 Jun;24(11):6338-6345. doi: 
      10.26355/eurrev_202006_21532.