PMID- 32573970
OWN - NLM
STAT- MEDLINE
DCOM- 20210527
LR  - 20210527
IS  - 2324-9269 (Electronic)
IS  - 2324-9269 (Linking)
VI  - 8
IP  - 9
DP  - 2020 Sep
TI  - Cytogenetic and FISH analysis of 93 multiple myeloma Moroccan patients.
PG  - e1363
LID - 10.1002/mgg3.1363 [doi]
LID - e1363
AB  - BACKGROUND: Multiple myeloma (MM) is a disease characterized by heterogeneous 
      clinical presentations as well as complex genetic and molecular abnormalities. In 
      MM, cytogenetic analysis is a challenge because of the low proliferation of 
      malignant plasma cells. Thus, interphase fluorescence in situ hybridization 
      (FISH), performed on sorted plasma cells detected abnormalities independently of 
      a proliferative and infiltrative index. The purpose of this study was to explore, 
      for the first time, the cytogenetic and molecular genetics features in Moroccan 
      patients with multiple myeloma referred exclusively to National Reference 
      Laboratory and to determine their risk stratification based on these features. 
      METHODS: We performed cytogenetic analysis on 93 MM cases, all patients were 
      subjected to FISH analysis, among which 45 patients have benefited from both FISH 
      analysis and standard karyotype. RESULTS: Karyotype was normal in 78% (35/45) 
      while, it was complex with varied structural and numerical abnormalities in 22% 
      (10/45) of all patients, among which Hyperdiploid karyotype was found in 9% 
      (n = 4 cases) and nonhyperdiploid in 13% (n = 6 cases). The most common numerical 
      abnormalities were gains of chromosomes 3, 5, 9, 15, and 19. Whole chromosome 
      losses were also frequent, affecting chromosomes X, 3, 14, 16 and 22. FISH 
      analysis detected abnormalities in 50% of cases. The translocation t(4;14) and 
      dup (1q) were the most frequent types of anomalies (14% and 13% respectively), 
      followed by (17p) deletion and 14q32/IGH translocations with an undetermined 
      origin (12% each) then the (1p) deletion (4%). For the normal karyotypes, FISH 
      revealed chromosome abnormalities in 46%. CONCLUSION: This study compares the 
      results of cytogenetic analysis of chromosomal abnormalities in the Moroccan 
      population with other countries. (1/2) patient showed at least one type of molecular 
      genetic abnormalities. Therefore, the introducing of the cytogenetic analysis is 
      obligatory in the diagnosis of multiple myeloma.
CI  - (c) 2020 The Authors. Molecular Genetics & Genomic Medicine published by Wiley 
      Periodicals LLC.
FAU - Hamdaoui, Hasna
AU  - Hamdaoui H
AUID- ORCID: 0000-0002-8267-8186
AD  - National Reference Laboratory, Mohammed VI University of Health Sciences (UM6SS), 
      Casablanca, Morocco.
AD  - Laboratory of Health and Environment, Faculty of Sciences Ain Chock, University 
      Hassan II, Casablanca, Morocco.
FAU - Benlarroubia, Oumaima
AU  - Benlarroubia O
AD  - National Reference Laboratory, Mohammed VI University of Health Sciences (UM6SS), 
      Casablanca, Morocco.
FAU - Ait Boujmia, Oum Kaltoum
AU  - Ait Boujmia OK
AUID- ORCID: 0000-0002-1014-9728
AD  - Laboratory of Cellular and Molecular Pathology, Faculty of Medicine and Pharmacy 
      of Casablanca, University Hassan II, Casablanca, Morocco.
FAU - Mossafa, Hossein
AU  - Mossafa H
AD  - National Reference Laboratory, Mohammed VI University of Health Sciences (UM6SS), 
      Casablanca, Morocco.
FAU - Ouldim, Karim
AU  - Ouldim K
AD  - Faculty of Medicine and Pharmacy, Medical Genetics and Oncogenetics Unit, Sidi 
      Mohamed Ben Abdellah University, Fes, Morocco.
FAU - Belkhayat, Aziza
AU  - Belkhayat A
AD  - National Reference Laboratory, Mohammed VI University of Health Sciences (UM6SS), 
      Casablanca, Morocco.
FAU - Smyej, Imane
AU  - Smyej I
AD  - National Reference Laboratory, Mohammed VI University of Health Sciences (UM6SS), 
      Casablanca, Morocco.
FAU - Benrahma, Houda
AU  - Benrahma H
AD  - National Reference Laboratory, Mohammed VI University of Health Sciences (UM6SS), 
      Casablanca, Morocco.
AD  - Faculty of Medicine, Mohammed VI University of Health Sciences (UM6SS), 
      Casablanca, Morocco.
FAU - Dehbi, Hind
AU  - Dehbi H
AD  - Laboratory of Cellular and Molecular Pathology, Faculty of Medicine and Pharmacy 
      of Casablanca, University Hassan II, Casablanca, Morocco.
FAU - Chegdani, Fatima
AU  - Chegdani F
AD  - Laboratory of Health and Environment, Faculty of Sciences Ain Chock, University 
      Hassan II, Casablanca, Morocco.
LA  - eng
PT  - Journal Article
DEP - 20200623
PL  - United States
TA  - Mol Genet Genomic Med
JT  - Molecular genetics & genomic medicine
JID - 101603758
SB  - IM
MH  - *Abnormal Karyotype
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Chromosome Deletion
MH  - Chromosome Duplication
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Multiple Myeloma/blood/*genetics
MH  - Translocation, Genetic
PMC - PMC7507047
OTO - NOTNLM
OT  - FISH
OT  - conventional karyotype
OT  - cytogenetic
OT  - multiple myeloma
OT  - plasma cells sorted
COIS- The authors declare that there are no conflicts of interest.
EDAT- 2020/06/24 06:00
MHDA- 2021/05/28 06:00
PMCR- 2020/06/23
CRDT- 2020/06/24 06:00
PHST- 2020/03/19 00:00 [received]
PHST- 2020/05/13 00:00 [revised]
PHST- 2020/05/19 00:00 [accepted]
PHST- 2020/06/24 06:00 [pubmed]
PHST- 2021/05/28 06:00 [medline]
PHST- 2020/06/24 06:00 [entrez]
PHST- 2020/06/23 00:00 [pmc-release]
AID - MGG31363 [pii]
AID - 10.1002/mgg3.1363 [doi]
PST - ppublish
SO  - Mol Genet Genomic Med. 2020 Sep;8(9):e1363. doi: 10.1002/mgg3.1363. Epub 2020 Jun 
      23.