PMID- 32576886
OWN - NLM
STAT- MEDLINE
DCOM- 20201130
LR  - 20210623
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 10
IP  - 1
DP  - 2020 Jun 23
TI  - Clinical relevance of B7H3 expression in retinoblastoma.
PG  - 10185
LID - 10.1038/s41598-020-67101-7 [doi]
LID - 10185
AB  - Retinoblastoma (RB) is the most common paediatric intraocular tumour. Currently, 
      chemotherapy is widely used to reduce the chance of metastasis as well as for 
      vision salvage. The limitations of chemotherapy for RB include chemoresistance 
      and cytotoxicity. Recently, immunotherapy is considered for treating 
      chemoresistant cancers. Although, several molecular targets are available for 
      immunotherapy in different cancers, we were interested in B7H3, as it was 
      differentially expressed between retinoblastoma and retina in our earlier 
      proteomics study. Hence, in this study we validated the previous finding by 
      Western blotting and immunohistochemistry on primary RB tumor samples. The 
      results suggest significantly increased expression of B7H3 in RB tumor samples 
      compared to retina by western blotting. Immunohistochemistry revealed spatial, 
      inter and intratumoral heterogeneity in the primary RB tumor sections. 
      Correlation of the B7H3 expression with clinical and histopathological data 
      revealed significantly increased expression of B7H3 in poorly differentiated, 
      non-neural invasive tumors and lower expression in neural invasion and severe 
      anaplastic areas of the tumors. B7H3 expression did not significantly vary 
      between low-risk and high-risk tumors. The study also revealed considerably 
      reduced infiltration of T lymphocytes in RB. We conclude that B7H3 is prominently 
      expressed in primary RB tumors and could be used for targeted therapy.
FAU - Ganesan, Bhuvaneswari
AU  - Ganesan B
AD  - L&T Ocular Pathology Department, Vision Research Foundation, Chennai, India.
FAU - Parameswaran, Sowmya
AU  - Parameswaran S
AD  - Radheshyam Kanoi Stem Cell Laboratory, Vision Research Foundation, Chennai, 
      India.
FAU - Sharma, Ashwani
AU  - Sharma A
AD  - Department of Chemistry & Biology, Indian Institute of Science Education and 
      Research (IISER), Tirupati, India.
FAU - Krishnakumar, Subramanian
AU  - Krishnakumar S
AD  - L&T Ocular Pathology Department, Vision Research Foundation, Chennai, India. 
      drkrishnakumar_2000@yahoo.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200623
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (B7 Antigens)
RN  - 0 (CD276 protein, human)
SB  - IM
MH  - B7 Antigens/*metabolism
MH  - Cell Differentiation/physiology
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - Humans
MH  - Immunohistochemistry/methods
MH  - Immunotherapy/methods
MH  - Infant
MH  - Male
MH  - Retinal Neoplasms/immunology/*metabolism/therapy
MH  - Retinoblastoma/immunology/*metabolism/therapy
MH  - T-Lymphocytes/metabolism
PMC - PMC7311428
COIS- The authors declare no competing interests.
EDAT- 2020/06/25 06:00
MHDA- 2020/12/01 06:00
PMCR- 2020/06/23
CRDT- 2020/06/25 06:00
PHST- 2019/12/12 00:00 [received]
PHST- 2020/05/22 00:00 [accepted]
PHST- 2020/06/25 06:00 [entrez]
PHST- 2020/06/25 06:00 [pubmed]
PHST- 2020/12/01 06:00 [medline]
PHST- 2020/06/23 00:00 [pmc-release]
AID - 10.1038/s41598-020-67101-7 [pii]
AID - 67101 [pii]
AID - 10.1038/s41598-020-67101-7 [doi]
PST - epublish
SO  - Sci Rep. 2020 Jun 23;10(1):10185. doi: 10.1038/s41598-020-67101-7.