PMID- 32578338
OWN - NLM
STAT- MEDLINE
DCOM- 20201013
LR  - 20230331
IS  - 1740-0929 (Electronic)
IS  - 1344-3941 (Print)
IS  - 1344-3941 (Linking)
VI  - 91
IP  - 1
DP  - 2020 Jan-Dec
TI  - Pluripotency-associated genes reposition during early embryonic developmental 
      stages in pigs.
PG  - e13408
LID - 10.1111/asj.13408 [doi]
LID - e13408
AB  - We examined the allelic expression and positioning of two pluripotency-associated 
      genes, OCT4 and SOX2, and two housekeeping genes, ACTB and TUBA, in 4- and 8-cell 
      porcine embryos utilizing RNA and DNA fluorescence in situ hybridization (FISH) 
      in single blastomeres. The proportion of blastomeres expressing SOX2 
      bi-allelically increased from 45% at the 4-cell stage to 60% at the 8-cell stage. 
      Moreover, in 8-cell embryos, SOX2 was expressed bi-allelically in significantly 
      more blastomeres than was the case for OCT4, and this was associated with a 
      tendency for SOX2 alleles to move toward the nuclear interior during 4- to 8-cell 
      transition. However, the radial location of OCT4 alleles did not change 
      significantly during this transition. The locations of active and inactive 
      alleles based on DNA and RNA FISH signals were also calculated. Inactive OCT4 
      alleles were located in very close proximity to the nuclear membrane, whereas 
      active OCT4 alleles were more centrally disposed in the nucleus. Nevertheless, 
      the nuclear location of active and inactive SOX2 alleles did not change in either 
      4- or 8-cell blastomeres. Our RNA and DNA FISH data provide novel information on 
      the allelic expression patterns and positioning of pluripotency-associated genes, 
      OCT4 and SOX2, during embryonic genome activation in pigs.
CI  - (c) 2020 The Authors. Animal Science Journal published by John Wiley & Sons 
      Australia, Ltd on behalf of Japanese Society of Animal Science.
FAU - Nguyen, Hiep Thi
AU  - Nguyen HT
AD  - Institute of Agrobiological Sciences, National Agriculture and Food Research 
      Organization (NARO), Tsukuba, Japan.
AD  - The United Graduate School of Veterinary Science, Yamaguchi University, 
      Yamaguchi, Japan.
AD  - Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, 
      Vietnam.
FAU - Nghia, Nguyen Trong
AU  - Nghia NT
AD  - Institute of Physics, Vietnam Academy of Science and Technology, Hanoi, Vietnam.
FAU - Lien, Nghiem Thi Ha
AU  - Lien NTH
AD  - Institute of Physics, Vietnam Academy of Science and Technology, Hanoi, Vietnam.
FAU - Dang-Nguyen, Thanh Quang
AU  - Dang-Nguyen TQ
AUID- ORCID: 0000-0001-8176-4827
AD  - Institute of Agrobiological Sciences, National Agriculture and Food Research 
      Organization (NARO), Tsukuba, Japan.
FAU - Men, Nguyen Thi
AU  - Men NT
AD  - Institute of Agrobiological Sciences, National Agriculture and Food Research 
      Organization (NARO), Tsukuba, Japan.
FAU - Viet Linh, Nguyen
AU  - Viet Linh N
AD  - Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, 
      Vietnam.
FAU - Xuan Nguyen, Bui
AU  - Xuan Nguyen B
AD  - Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, 
      Vietnam.
FAU - Noguchi, Junko
AU  - Noguchi J
AD  - Institute of Agrobiological Sciences, National Agriculture and Food Research 
      Organization (NARO), Tsukuba, Japan.
FAU - Kaneko, Hiroyuki
AU  - Kaneko H
AUID- ORCID: 0000-0003-1829-3895
AD  - Institute of Agrobiological Sciences, National Agriculture and Food Research 
      Organization (NARO), Tsukuba, Japan.
FAU - Kikuchi, Kazuhiro
AU  - Kikuchi K
AUID- ORCID: 0000-0002-7198-9237
AD  - Institute of Agrobiological Sciences, National Agriculture and Food Research 
      Organization (NARO), Tsukuba, Japan.
AD  - The United Graduate School of Veterinary Science, Yamaguchi University, 
      Yamaguchi, Japan.
LA  - eng
GR  - JPMJSA1404/Science and Technology Research Partnership for Sustainable 
      Development/International
PT  - Journal Article
PL  - Australia
TA  - Anim Sci J
JT  - Animal science journal = Nihon chikusan Gakkaiho
JID - 100956805
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (DNMBP protein, human)
RN  - 0 (Octamer Transcription Factor-3)
RN  - 0 (POU5F1 protein, human)
RN  - 0 (SOX2 protein, human)
RN  - 0 (SOXB1 Transcription Factors)
SB  - IM
MH  - Alleles
MH  - Animals
MH  - Blastomeres/*cytology/*metabolism
MH  - Cytoskeletal Proteins/genetics/metabolism
MH  - Embryonic Development/*genetics
MH  - Fertilization in Vitro
MH  - *Gene Expression
MH  - *Gene Expression Regulation, Developmental
MH  - In Situ Hybridization, Fluorescence
MH  - In Vitro Oocyte Maturation Techniques
MH  - In Vitro Techniques
MH  - Octamer Transcription Factor-3/genetics/metabolism
MH  - SOXB1 Transcription Factors/genetics/metabolism
MH  - Swine/*embryology/*genetics
PMC - PMC7378944
OTO - NOTNLM
OT  - allelic expression
OT  - gene positioning
OT  - pluripotency-associated genes
OT  - porcine embryos
COIS- All authors declare no conflict of interest.
EDAT- 2020/06/25 06:00
MHDA- 2020/10/21 06:00
PMCR- 2020/07/24
CRDT- 2020/06/25 06:00
PHST- 2020/04/07 00:00 [received]
PHST- 2020/05/13 00:00 [revised]
PHST- 2020/05/22 00:00 [accepted]
PHST- 2020/06/25 06:00 [entrez]
PHST- 2020/06/25 06:00 [pubmed]
PHST- 2020/10/21 06:00 [medline]
PHST- 2020/07/24 00:00 [pmc-release]
AID - ASJ13408 [pii]
AID - 10.1111/asj.13408 [doi]
PST - ppublish
SO  - Anim Sci J. 2020 Jan-Dec;91(1):e13408. doi: 10.1111/asj.13408.