PMID- 32578983
OWN - NLM
STAT- MEDLINE
DCOM- 20201204
LR  - 20201214
IS  - 1520-510X (Electronic)
IS  - 0020-1669 (Linking)
VI  - 59
IP  - 13
DP  - 2020 Jul 6
TI  - Copper Complexes with 1,10-Phenanthroline Derivatives: Underlying Factors 
      Affecting Their Cytotoxicity.
PG  - 9116-9134
LID - 10.1021/acs.inorgchem.0c00925 [doi]
AB  - The interpretation of in vitro cytotoxicity data of Cu(II)-1,10-phenanthroline 
      (phen) complexes normally does not take into account the speciation that 
      complexes undergo in cell incubation media and its implications in cellular 
      uptake and mechanisms of action. We synthesize and test the activity of several 
      distinct Cu(II)-phen compounds; up to 24 h of incubation, the cytotoxic activity 
      differs for the Cu complexes and the corresponding free ligands, but for longer 
      incubation times (e.g., 72 h), all compounds display similar activity. Combining 
      the use of several spectroscopic, spectrometric, and electrochemical techniques, 
      the speciation of Cu-phen compounds in cell incubation media is evaluated, 
      indicating that the originally added complex almost totally decomposed and that 
      Cu(II) and phen are mainly bound to bovine serum albumin. Several methods are 
      used to disclose relationships between structure, activity, speciation in 
      incubation media, cellular uptake, distribution of Cu in cells, and cytotoxicity. 
      Contrary to what is reported in most studies, we conclude that interaction with 
      cell components and cell death involves the separate action of Cu ions and phen 
      molecules, not [Cu(phen)(n)] species. This conclusion should similarly apply to 
      many other Cu-ligand systems reported to date.
FAU - Nunes, Patrique
AU  - Nunes P
AD  - Centro de Quimica Estrutural and Departamento de Engenharia Quimica, Instituto 
      Superior Tecnico, Universidade de Lisboa, Av Rovisco Pais, 1049-001 Lisboa, 
      Portugal.
FAU - Correia, Isabel
AU  - Correia I
AD  - Centro de Quimica Estrutural and Departamento de Engenharia Quimica, Instituto 
      Superior Tecnico, Universidade de Lisboa, Av Rovisco Pais, 1049-001 Lisboa, 
      Portugal.
FAU - Marques, Fernanda
AU  - Marques F
AD  - Centro de Ciencias e Tecnologias Nucleares and Departamento de Ciencias e 
      Engenharia Nucleares, Instituto Superior Tecnico, Universidade de Lisboa, Estrada 
      Nacional 10, 2695-066 Bobadela LRS, Portugal.
FAU - Matos, Antonio Pedro
AU  - Matos AP
AD  - Centro de Investigacao Interdisciplinar Egas Moniz, Campus Universitario, Quinta 
      da Granja, Monte de Caparica, 2829-511 Caparica, Portugal.
FAU - Dos Santos, Margarida M C
AU  - Dos Santos MMC
AD  - Centro de Quimica Estrutural and Departamento de Engenharia Quimica, Instituto 
      Superior Tecnico, Universidade de Lisboa, Av Rovisco Pais, 1049-001 Lisboa, 
      Portugal.
FAU - Azevedo, Cristina G
AU  - Azevedo CG
AD  - Centro de Quimica Estrutural and Departamento de Engenharia Quimica, Instituto 
      Superior Tecnico, Universidade de Lisboa, Av Rovisco Pais, 1049-001 Lisboa, 
      Portugal.
FAU - Capelo, Jose-Luis
AU  - Capelo JL
AD  - LAVQ, REQUIMTE, Departamento de Quimica, Faculdade de Ciencias e Tecnologia, 
      Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
AD  - PROTEOMASS Scientific Society, Madan Park, Rua dos Inventores, 2825-152 Caparica, 
      Portugal.
FAU - Santos, Hugo M
AU  - Santos HM
AD  - LAVQ, REQUIMTE, Departamento de Quimica, Faculdade de Ciencias e Tecnologia, 
      Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
AD  - PROTEOMASS Scientific Society, Madan Park, Rua dos Inventores, 2825-152 Caparica, 
      Portugal.
FAU - Gama, Sofia
AU  - Gama S
AD  - Department of Analytical Chemistry, Faculty of Chemistry, University of 
      Bialystok, ul. Ciolkowskiego 1K, 15-245 Bialystok, Poland.
FAU - Pinheiro, Teresa
AU  - Pinheiro T
AD  - Institute for Bioengineering and Biosciences and Departamento de Engenharia e 
      Ciencias Nucleares, Instituto Superior Tecnico, Universidade de Lisboa, Av. 
      Rovisco Pais 1, 1049-001 Lisboa, Portugal.
FAU - Cavaco, Isabel
AU  - Cavaco I
AD  - Centro de Quimica Estrutural and Departamento de Engenharia Quimica, Instituto 
      Superior Tecnico, Universidade de Lisboa, Av Rovisco Pais, 1049-001 Lisboa, 
      Portugal.
AD  - Departamento de Quimica e Farmacia, Universidade do Algarve, Campus de Gambelas, 
      8005-139 Faro, Portugal.
FAU - Pessoa, Joao Costa
AU  - Pessoa JC
AUID- ORCID: 0000-0002-3978-9964
AD  - Centro de Quimica Estrutural and Departamento de Engenharia Quimica, Instituto 
      Superior Tecnico, Universidade de Lisboa, Av Rovisco Pais, 1049-001 Lisboa, 
      Portugal.
LA  - eng
PT  - Journal Article
DEP - 20200624
PL  - United States
TA  - Inorg Chem
JT  - Inorganic chemistry
JID - 0366543
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Coordination Complexes)
RN  - 0 (Ligands)
RN  - 0 (Phenanthrolines)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - 789U1901C5 (Copper)
SB  - IM
MH  - Animals
MH  - Antineoplastic Agents/chemical synthesis/metabolism/*pharmacology
MH  - Cattle
MH  - Cell Line, Tumor
MH  - Coordination Complexes/chemical synthesis/metabolism/*pharmacology
MH  - Copper/chemistry/metabolism/*pharmacology
MH  - Drug Screening Assays, Antitumor
MH  - Humans
MH  - Ligands
MH  - Phenanthrolines/chemical synthesis/metabolism/*pharmacology
MH  - Protein Binding
MH  - Serum Albumin, Bovine/metabolism
EDAT- 2020/06/25 06:00
MHDA- 2020/12/15 06:00
CRDT- 2020/06/25 06:00
PHST- 2020/06/25 06:00 [pubmed]
PHST- 2020/12/15 06:00 [medline]
PHST- 2020/06/25 06:00 [entrez]
AID - 10.1021/acs.inorgchem.0c00925 [doi]
PST - ppublish
SO  - Inorg Chem. 2020 Jul 6;59(13):9116-9134. doi: 10.1021/acs.inorgchem.0c00925. Epub 
      2020 Jun 24.