PMID- 32580933
OWN - NLM
STAT- MEDLINE
DCOM- 20210315
LR  - 20210315
IS  - 1550-6606 (Electronic)
IS  - 0022-1767 (Linking)
VI  - 205
IP  - 3
DP  - 2020 Aug 1
TI  - Extracellular Release of Antigen by Dendritic Cell Regurgitation Promotes B Cell 
      Activation through NF-kappaB/cRel.
PG  - 608-618
LID - 10.4049/jimmunol.1900394 [doi]
AB  - Dendritic cells (DCs) are professional APCs, which sample Ags in the periphery 
      and migrate to the lymph node where they activate T cells. DCs can also present 
      native Ag to B cells through interactions observed both in vitro and in vivo. 
      However, the mechanisms of Ag transfer and B cell activation by DCs remain 
      incompletely understood. In this study, we report that murine DCs are an 
      important cell transporter of Ag from the periphery to the lymph node B cell zone 
      and also potent inducers of B cell activation both in vivo and in vitro. 
      Importantly, we highlight a novel extracellular mechanism of B cell activation by 
      DCs. In this study, we demonstrate that Ag released upon DC regurgitation is 
      sufficient to efficiently induce early B cell activation, which is BCR driven and 
      mechanistically dependent on the nuclear accumulation of the transcription factor 
      NF-kappaB/cRel. Thus, our study provides new mechanistic insights into Ag delivery 
      and B cell activation modalities by DCs and a promising approach for targeting 
      NF-kappaB/cRel pathway to modulate the DC-elicited B cell responses.
CI  - Copyright (c) 2020 by The American Association of Immunologists, Inc.
FAU - El-Barbry, Houssam
AU  - El-Barbry H
AD  - Universite de Paris, Institut Cochin, INSERM, U1016, CNRS, UMR 8104, F-75014 
      Paris, France.
FAU - Capitao, Marisa
AU  - Capitao M
AD  - Universite de Paris, Institut Cochin, INSERM, U1016, CNRS, UMR 8104, F-75014 
      Paris, France.
FAU - Barrin, Sarah
AU  - Barrin S
AD  - Universite de Paris, Institut Cochin, INSERM, U1016, CNRS, UMR 8104, F-75014 
      Paris, France.
FAU - Amziani, Samir
AU  - Amziani S
AD  - Universite de Paris, Institut Cochin, INSERM, U1016, CNRS, UMR 8104, F-75014 
      Paris, France.
FAU - Pierre Paul, Pascal
AU  - Pierre Paul P
AD  - Universite de Paris, Institut Cochin, INSERM, U1016, CNRS, UMR 8104, F-75014 
      Paris, France.
FAU - Borreill, Susanna
AU  - Borreill S
AD  - Universite de Paris, Institut Cochin, INSERM, U1016, CNRS, UMR 8104, F-75014 
      Paris, France.
FAU - Guilbert, Thomas
AU  - Guilbert T
AD  - Universite de Paris, Institut Cochin, INSERM, U1016, CNRS, UMR 8104, F-75014 
      Paris, France.
FAU - Donnadieu, Emmanuel
AU  - Donnadieu E
AD  - Universite de Paris, Institut Cochin, INSERM, U1016, CNRS, UMR 8104, F-75014 
      Paris, France.
FAU - Niedergang, Florence
AU  - Niedergang F
AUID- ORCID: 0000-0002-7064-830X
AD  - Universite de Paris, Institut Cochin, INSERM, U1016, CNRS, UMR 8104, F-75014 
      Paris, France.
FAU - Ouaaz, Fatah
AU  - Ouaaz F
AUID- ORCID: 0000-0001-8881-5703
AD  - Universite de Paris, Institut Cochin, INSERM, U1016, CNRS, UMR 8104, F-75014 
      Paris, France fatah.ouaaz@inserm.fr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200624
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antigens)
RN  - 0 (NF-kappa B)
RN  - 0 (Proto-Oncogene Proteins c-rel)
SB  - IM
MH  - Animals
MH  - *Antigen Presentation
MH  - Antigens/genetics/*immunology
MH  - B-Lymphocytes/*immunology
MH  - Dendritic Cells/*immunology
MH  - Female
MH  - *Lymphocyte Activation
MH  - Mice
MH  - Mice, Transgenic
MH  - NF-kappa B/genetics/*immunology
MH  - Proto-Oncogene Proteins c-rel/genetics/*immunology
MH  - Signal Transduction/*immunology
EDAT- 2020/06/26 06:00
MHDA- 2021/03/16 06:00
CRDT- 2020/06/26 06:00
PHST- 2019/04/04 00:00 [received]
PHST- 2020/05/03 00:00 [accepted]
PHST- 2020/06/26 06:00 [pubmed]
PHST- 2021/03/16 06:00 [medline]
PHST- 2020/06/26 06:00 [entrez]
AID - jimmunol.1900394 [pii]
AID - 10.4049/jimmunol.1900394 [doi]
PST - ppublish
SO  - J Immunol. 2020 Aug 1;205(3):608-618. doi: 10.4049/jimmunol.1900394. Epub 2020 
      Jun 24.