PMID- 32583437
OWN - NLM
STAT- MEDLINE
DCOM- 20210908
LR  - 20210908
IS  - 1097-4652 (Electronic)
IS  - 0021-9541 (Linking)
VI  - 236
IP  - 2
DP  - 2021 Feb
TI  - Effects of photobiomodulation combined with MSCs transplantation on the repair of 
      spinal cord injury in rat.
PG  - 921-930
LID - 10.1002/jcp.29902 [doi]
AB  - Stem cell transplantation has shown promising regenerative effects against neural 
      injury, and photobiomodulation (PBM) can aid tissue recovery. This study aims to 
      evaluate the therapeutic effect of human umbilical cord mesenchymal stem cells 
      (hUCMSCs) and laser alone or combined on spinal cord injury (SCI). The animals 
      were divided into SCI, hUCMSCs, laser treatment (LASER) and combination treatment 
      (hUCMSCs + LASER) groups. Cell-enriched grafts of hUCMSCs (1 x 10(6) cells/ml) 
      were injected at the site of antecedent trauma in SCI model rats. A 2 cm(2) 
      damaged area was irradiated with 630 nm laser at 100 mW/cm(2) power for 20 min. 
      Locomotion was evaluated using Basso-Beattie-Bresnahan (BBB) scores, and 
      neurofilament repair were monitored by histological staining and diffusion tensor 
      imaging (DTI). First, after SCI, the motor function of each group was restored 
      with different degrees, the combination treatment significantly increased the BBB 
      scores compared to either monotherapy. In addition, Nissl bodies were more 
      numerous, and the nerve fibers were longer and thicker in the combination 
      treatment group. Consistent with this, the in situ expression of NF-200 and glial 
      fibrillary acidic protein in the damaged area was the highest in the combination 
      treatment group. Finally, DTI showed that the combination therapy optimally 
      improved neurofilament structure and arrangement. These results may show that the 
      combination of PBM and hUCMSCs transplantation is a feasible strategy for 
      reducing secondary damage and promoting functional recovery following SCI.
CI  - (c) 2020 Wiley Periodicals LLC.
FAU - Chen, Hongli
AU  - Chen H
AD  - School of Life Sciences, Tiangong University, Tianjin, China.
AD  - Academy of Medical Engineering and Translational Medicine, Tianjin University, 
      Tianjin, China.
FAU - Wang, Yunhao
AU  - Wang Y
AD  - School of Life Sciences, Tiangong University, Tianjin, China.
FAU - Tu, Wenjun
AU  - Tu W
AD  - School of Life Sciences, Tiangong University, Tianjin, China.
AD  - Institute of Radiation Medicine, Chinese Academy of Medical Science & Peking 
      Union Medical College, Tianjin, China.
FAU - Wang, Hong
AU  - Wang H
AUID- ORCID: 0000-0001-5580-707X
AD  - School of Life Sciences, Tiangong University, Tianjin, China.
AD  - Institute of Radiation Medicine, Chinese Academy of Medical Science & Peking 
      Union Medical College, Tianjin, China.
FAU - Yin, Huijuan
AU  - Yin H
AD  - Institute of Biomedical Engineering, Chinese Academy of Medical Science & Peking 
      Union Medical College, Tianjin, China.
FAU - Sha, Hong
AU  - Sha H
AD  - Institute of Biomedical Engineering, Chinese Academy of Medical Science & Peking 
      Union Medical College, Tianjin, China.
FAU - Li, Yingxin
AU  - Li Y
AD  - Institute of Biomedical Engineering, Chinese Academy of Medical Science & Peking 
      Union Medical College, Tianjin, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200624
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
SB  - IM
MH  - Animals
MH  - Cell Differentiation/radiation effects
MH  - Cells, Cultured
MH  - Diffusion Tensor Imaging/methods
MH  - Humans
MH  - Intermediate Filaments/radiation effects
MH  - Low-Level Light Therapy/methods
MH  - Male
MH  - Mesenchymal Stem Cell Transplantation/methods
MH  - Mesenchymal Stem Cells/*physiology
MH  - Motor Activity/radiation effects
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Recovery of Function/radiation effects
MH  - Spinal Cord/radiation effects
MH  - Spinal Cord Injuries/*radiotherapy/*therapy
MH  - Umbilical Cord/radiation effects
OTO - NOTNLM
OT  - PBM
OT  - functional recovery
OT  - mesenchymal stem cell
OT  - spinal cord injury
OT  - transplantation
EDAT- 2020/06/26 06:00
MHDA- 2021/09/09 06:00
CRDT- 2020/06/26 06:00
PHST- 2020/01/20 00:00 [received]
PHST- 2020/06/14 00:00 [revised]
PHST- 2020/06/14 00:00 [accepted]
PHST- 2020/06/26 06:00 [pubmed]
PHST- 2021/09/09 06:00 [medline]
PHST- 2020/06/26 06:00 [entrez]
AID - 10.1002/jcp.29902 [doi]
PST - ppublish
SO  - J Cell Physiol. 2021 Feb;236(2):921-930. doi: 10.1002/jcp.29902. Epub 2020 Jun 
      24.