PMID- 32585218
OWN - NLM
STAT- MEDLINE
DCOM- 20200826
LR  - 20200826
IS  - 1879-3150 (Electronic)
IS  - 0041-0101 (Linking)
VI  - 184
DP  - 2020 Sep
TI  - Disorders on cardiovascular parameters in rats and in human blood cells caused by 
      Lachesis acrochorda snake venom.
PG  - 180-191
LID - S0041-0101(20)30288-9 [pii]
LID - 10.1016/j.toxicon.2020.06.009 [doi]
AB  - In Colombia, Lachesis acrochorda causes 2-3% of all snake envenomations. The 
      accidents promote a high mortality rate (90%) due to blood and cardiovascular 
      complications. Here, the effects of the snake venom of L. acrochorda (SVLa) were 
      analyzed on human blood cells and on cardiovascular parameters of rats. SVLa 
      induced blood coagulation, as measured by the prothrombin time test, but did not 
      reduce the cell viability of neutrophils and platelets evaluated by the 
      3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide (MTT) reduction 
      assay and by the lactate dehydrogenase (LDH) enzyme assay. In fact, SVLa 
      increased the absorbance in tests made with platelets subjected to the MTT assay. 
      SVLa induced platelet aggregation whose magnitude was comparable to that of the 
      positive control adenosine diphosphate (ADP), and occurred earlier with 
      increasing SVLa concentration. Acetylsalicylic acid (ASA, a cyclooxygenase 
      inhibitor) or clopidogrel (an ADP receptor blocker) inhibited the aggregating 
      effect of SVLa. Inhibition of SVLa-elicited platelet aggregation also resulted 
      from the treatment with disodium ethylenediaminetetraacetate (Na(2)-EDTA; 
      metalloproteinase inhibitor) and with 4-(2-aminoethyl) benzenesulfonyl fluoride 
      hydrochloride (AEBSF, serine protease inhibitor). In isolated right atrium of 
      rats, SVLa increased slightly, but significantly, the magnitude of the 
      spontaneous contractions and, in isolated rat aorta, SVLa relaxed KCl- or 
      phenylephrine-induced contractions. In vivo, SVLa induced hypotension and 
      bradycardia in rats, with detection of hemorrhage in pulmonary and renal tissues. 
      Altogether, under experimental conditions, SVLa induced blood coagulation, 
      platelet aggregation, hypotension and bradycardia. Part of the effects presented 
      here may be explained by the presence of snake venom metalloproteinases (SVMPs) 
      and snake venom serine proteases (SVSPs), constituents of SVLa.
CI  - Copyright (c) 2020 Elsevier Ltd. All rights reserved.
FAU - Angel-Camilo, Karen Leonor
AU  - Angel-Camilo KL
AD  - Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade 
      Federal Do Ceara, Fortaleza, Ceara, Brazil; Facultad de Ciencias Naturales, 
      Exactas y de la Educacion, Departamento de Biologia, Centro de Investigaciones 
      Biomedicas-Bioterio, Grupo de Investigaciones Herpetologicas y Toxinologicas, 
      Universidad del Cauca, Popayan, Colombia.
FAU - Guerrero-Vargas, Jimmy Alexander
AU  - Guerrero-Vargas JA
AD  - Facultad de Ciencias Naturales, Exactas y de la Educacion, Departamento de 
      Biologia, Centro de Investigaciones Biomedicas-Bioterio, Grupo de Investigaciones 
      Herpetologicas y Toxinologicas, Universidad del Cauca, Popayan, Colombia.
FAU - Carvalho, Emanuella Feitosa de
AU  - Carvalho EF
AD  - Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade 
      Federal Do Ceara, Fortaleza, Ceara, Brazil.
FAU - Lima-Silva, Karine
AU  - Lima-Silva K
AD  - Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade 
      Federal Do Ceara, Fortaleza, Ceara, Brazil.
FAU - de Siqueira, Rodrigo Jose Bezerra
AU  - de Siqueira RJB
AD  - Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade 
      Federal Do Ceara, Fortaleza, Ceara, Brazil.
FAU - Freitas, Lyara Barbosa Nogueira
AU  - Freitas LBN
AD  - Centro de Estudos Farmaceuticos e Cosmeticos, Faculdade de Farmacia, Odontologia 
      e Enfermagem, Universidade Federal Do Ceara, Fortaleza, Ceara, Brazil.
FAU - Sousa, Joao Antonio Costa de
AU  - Sousa JAC
AD  - Centro de Estudos Farmaceuticos e Cosmeticos, Faculdade de Farmacia, Odontologia 
      e Enfermagem, Universidade Federal Do Ceara, Fortaleza, Ceara, Brazil.
FAU - Mota, Mario Rogerio Lima
AU  - Mota MRL
AD  - Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade 
      Federal Do Ceara, Fortaleza, Ceara, Brazil.
FAU - Santos, Armenio Aguiar Dos
AU  - Santos AAD
AD  - Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade 
      Federal Do Ceara, Fortaleza, Ceara, Brazil.
FAU - Neves-Ferreira, Ana Gisele da Costa
AU  - Neves-Ferreira AGDC
AD  - Laboratorio de Toxinologia, Instituto Oswaldo Cruz, Fiocruz, Fiocruz, Av. Brasil 
      4365, Manguinhos, Rio de Janeiro, 21040-900, Brazil.
FAU - Havt, Alexandre
AU  - Havt A
AD  - Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade 
      Federal Do Ceara, Fortaleza, Ceara, Brazil.
FAU - Leal, Luzia Kalyne Almeida Moreira
AU  - Leal LKAM
AD  - Centro de Estudos Farmaceuticos e Cosmeticos, Faculdade de Farmacia, Odontologia 
      e Enfermagem, Universidade Federal Do Ceara, Fortaleza, Ceara, Brazil.
FAU - Magalhaes, Pedro Jorge Caldas
AU  - Magalhaes PJC
AD  - Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade 
      Federal Do Ceara, Fortaleza, Ceara, Brazil. Electronic address: pjcmagal@ufc.br.
LA  - eng
PT  - Journal Article
DEP - 20200623
PL  - England
TA  - Toxicon
JT  - Toxicon : official journal of the International Society on Toxinology
JID - 1307333
RN  - 0 (Serine Proteinase Inhibitors)
RN  - 0 (Viper Venoms)
RN  - 9001-32-5 (Fibrinogen)
RN  - EC 3.4.- (Metalloproteases)
RN  - EC 3.4.- (Serine Proteases)
RN  - EC 3.4.21.- (Serine Endopeptidases)
SB  - IM
MH  - Animals
MH  - Blood Cells
MH  - Blood Coagulation
MH  - Blood Platelets
MH  - Cardiovascular System/*drug effects
MH  - Colombia
MH  - Fibrinogen
MH  - Hemorrhage
MH  - Humans
MH  - Hypotension
MH  - Metalloproteases
MH  - Platelet Aggregation
MH  - Prothrombin Time
MH  - Rats
MH  - Serine Endopeptidases
MH  - Serine Proteases
MH  - Serine Proteinase Inhibitors
MH  - Snake Bites
MH  - Viper Venoms/*toxicity
MH  - *Viperidae
OTO - NOTNLM
OT  - Hypotension
OT  - Lachesis
OT  - Snake venom
OT  - Vasodilation
OT  - platelet Aggregation
EDAT- 2020/06/26 06:00
MHDA- 2020/08/28 06:00
CRDT- 2020/06/26 06:00
PHST- 2020/01/31 00:00 [received]
PHST- 2020/06/11 00:00 [revised]
PHST- 2020/06/15 00:00 [accepted]
PHST- 2020/06/26 06:00 [pubmed]
PHST- 2020/08/28 06:00 [medline]
PHST- 2020/06/26 06:00 [entrez]
AID - S0041-0101(20)30288-9 [pii]
AID - 10.1016/j.toxicon.2020.06.009 [doi]
PST - ppublish
SO  - Toxicon. 2020 Sep;184:180-191. doi: 10.1016/j.toxicon.2020.06.009. Epub 2020 Jun 
      23.