PMID- 32585357
OWN - NLM
STAT- MEDLINE
DCOM- 20210311
LR  - 20210311
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 104
DP  - 2020 Sep
TI  - Two types of TNF-alpha and their receptors in snakehead (Channa argus): Functions in 
      antibacterial innate immunity.
PG  - 470-477
LID - S1050-4648(20)30385-5 [pii]
LID - 10.1016/j.fsi.2020.05.059 [doi]
AB  - Tumor necrosis factor-alpha (TNF-alpha) is a pluripotent mediator of pro-inflammatory and 
      antimicrobial defense mechanisms and a regulator of lymphoid organ development. 
      Although two types of TNF-alpha have been identified in several teleost species, 
      their functions in pathogen infection remain largely unexplored, especially in 
      pathogen clearance. Herein, we cloned and characterized two types of TNF-alpha, 
      termed shTNF-alpha1 and shTNF-alpha2, and their receptors, shTNFR1 and shTNFR2, from 
      snakehead (Channa argus). These genes were constitutively expressed in all tested 
      tissues, and were induced by Aeromonas schubertii and Nocardia seriolae in head 
      kidney and spleen in vivo, and by lipoteichoic acid (LTA), lipopolysaccharides 
      (LPS), and Polyinosinic-polycytidylic acid [Poly (I:C)] in head kidney leukocytes 
      (HKLs) in vitro. Moreover, recombinant shTNF-alpha1 and shTNF-alpha2 upregulated the 
      expression of endogenous shTNF-alpha1, shTNF-alpha2, shTNFR1, and shTNFR2, and enhanced 
      intracellular bactericidal activity, with shTNF-alpha1 having a greater effect than 
      shTNF-alpha2. These findings suggest important roles of fish TNFalpha1, TNFalpha2, and their 
      receptors in bacterial infection and pathogen clearance, and provide a new 
      insight into their function in antibacterial innate immunity.
CI  - Copyright (c) 2020 Elsevier Ltd. All rights reserved.
FAU - Cui, Zheng-Wei
AU  - Cui ZW
AD  - Key Laboratory of Fishery Drug Development of Ministry of Agriculture and Rural 
      Affairs, Key Laboratory of Aquatic Animal Immune Technology of Guangdong 
      Province, Pearl River Fisheries Research Institute, Chinese Academy of Fishery 
      Sciences, Guangzhou, China.
FAU - Kong, Lu-Lu
AU  - Kong LL
AD  - Key Laboratory of Fishery Drug Development of Ministry of Agriculture and Rural 
      Affairs, Key Laboratory of Aquatic Animal Immune Technology of Guangdong 
      Province, Pearl River Fisheries Research Institute, Chinese Academy of Fishery 
      Sciences, Guangzhou, China; College of Fisheries and Life Science, Shanghai Ocean 
      University, Shanghai, China.
FAU - Zhao, Fei
AU  - Zhao F
AD  - Key Laboratory of Fishery Drug Development of Ministry of Agriculture and Rural 
      Affairs, Key Laboratory of Aquatic Animal Immune Technology of Guangdong 
      Province, Pearl River Fisheries Research Institute, Chinese Academy of Fishery 
      Sciences, Guangzhou, China. Electronic address: zhaofei@prfri.ac.cn.
FAU - Tan, Ai-Ping
AU  - Tan AP
AD  - Key Laboratory of Fishery Drug Development of Ministry of Agriculture and Rural 
      Affairs, Key Laboratory of Aquatic Animal Immune Technology of Guangdong 
      Province, Pearl River Fisheries Research Institute, Chinese Academy of Fishery 
      Sciences, Guangzhou, China.
FAU - Deng, Yu-Ting
AU  - Deng YT
AD  - Key Laboratory of Fishery Drug Development of Ministry of Agriculture and Rural 
      Affairs, Key Laboratory of Aquatic Animal Immune Technology of Guangdong 
      Province, Pearl River Fisheries Research Institute, Chinese Academy of Fishery 
      Sciences, Guangzhou, China.
FAU - Jiang, Lan
AU  - Jiang L
AD  - Key Laboratory of Fishery Drug Development of Ministry of Agriculture and Rural 
      Affairs, Key Laboratory of Aquatic Animal Immune Technology of Guangdong 
      Province, Pearl River Fisheries Research Institute, Chinese Academy of Fishery 
      Sciences, Guangzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20200622
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Fish Proteins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Receptors, Tumor Necrosis Factor)
RN  - 0 (Teichoic Acids)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 56411-57-5 (lipoteichoic acid)
RN  - O84C90HH2L (Poly I-C)
RN  - Aeromonas schubertii
RN  - Nocardia seriolae
SB  - IM
MH  - Aeromonas/physiology
MH  - Animals
MH  - Fish Diseases/*immunology
MH  - Fish Proteins/genetics/immunology
MH  - Fishes/*genetics/*immunology
MH  - Gram-Negative Bacterial Infections/immunology/veterinary
MH  - Head Kidney/immunology
MH  - Immunity, Innate/*genetics
MH  - Leukocytes/immunology
MH  - Lipopolysaccharides/pharmacology
MH  - Nocardia/physiology
MH  - Nocardia Infections/immunology/veterinary
MH  - Poly I-C/pharmacology
MH  - Receptors, Tumor Necrosis Factor/*genetics/immunology
MH  - Spleen/immunology
MH  - Teichoic Acids/pharmacology
MH  - Tumor Necrosis Factor-alpha/*genetics/immunology
OTO - NOTNLM
OT  - Aeromonas schubertii
OT  - Nocardia seriolae
OT  - Snakehead
OT  - TNF-alpha
OT  - TNFR
EDAT- 2020/06/26 06:00
MHDA- 2021/03/12 06:00
CRDT- 2020/06/26 06:00
PHST- 2020/02/25 00:00 [received]
PHST- 2020/05/15 00:00 [revised]
PHST- 2020/05/23 00:00 [accepted]
PHST- 2020/06/26 06:00 [pubmed]
PHST- 2021/03/12 06:00 [medline]
PHST- 2020/06/26 06:00 [entrez]
AID - S1050-4648(20)30385-5 [pii]
AID - 10.1016/j.fsi.2020.05.059 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2020 Sep;104:470-477. doi: 10.1016/j.fsi.2020.05.059. 
      Epub 2020 Jun 22.