PMID- 32588307 OWN - NLM STAT- MEDLINE DCOM- 20200828 LR - 20211221 IS - 1614-7499 (Electronic) IS - 0944-1344 (Linking) VI - 27 IP - 28 DP - 2020 Oct TI - Ameliorative effect of kefir against gamma-irradiation induced liver injury in male rats: impact on oxidative stress and inflammation. PG - 35161-35173 LID - 10.1007/s11356-020-09833-7 [doi] AB - Ionizing radiation is a double-edged sword because of its benefits and risks to human health. Therefore, protecting human organs from harmful effects of radiation is an important concern of researchers. Kefir, as a good source of probiotics, received growing interest in protective medicine owing to its antioxidant, anti-inflammatory, and immunomodulatory properties. Thus, this study was planned to investigate the protective role of kefir against gamma-radiation-induced hepatotoxicity. Thirty-two male rats were distributed in four groups: (I) control, (II) received Kefir orally (5 ml/kg body weight) for 28 days, (III) exposed to whole body gamma-irradiation (6.5 Gy) to induce hepatotoxicity, and (IV) was pretreated with kefir for 21 days then exposed to gamma-irradiation followed by 7 days of kefir treatment. At the end of the experiment, complete blood picture (CBC), liver function, and lipid profile were estimated. Furthermore, levels of lipid peroxidation, nitric oxide content, and endogenous antioxidants, in addition to concentrations of copper, iron, and calcium were measured in liver tissue. Furthermore, monocyte chemoattractant protein-1 (MCP-1) and relative gene expression of nuclear factor kappa (NF-kappaB) were assessed. The results revealed that oral administration of kefir significantly reduced the radiation-induced hepatic histological alterations, hepatic function impairment, and dyslipidemia. Moreover, kefir notably ameliorated the state of oxidative stress and appeared to inhibit the induced inflammation. This study provides a possible counteracting role of kefir against hepatotoxicity induced gamma-radiation. This can focus the benefit of kefir application as a prophylactic treatment to limit hepatic inflammation during radiotherapy. FAU - Ali, Ola Sayed M AU - Ali OSM AD - Department of Biochemistry, Faculty of Pharmacy (Girls), Al Azhar University, Nasr City, Cairo, Egypt. FAU - Amin, Nour El-Din AU - Amin NE AD - Department of Drug Radiation Research, National Centre for Radiation Research and Technology (NCRRT) Atomic Energy Authority, Nasr City, Cairo, 11137, Egypt. FAU - Abdel Fattah, Salma M AU - Abdel Fattah SM AD - Department of Drug Radiation Research, National Centre for Radiation Research and Technology (NCRRT) Atomic Energy Authority, Nasr City, Cairo, 11137, Egypt. FAU - Abd El-Rahman, Omnia AU - Abd El-Rahman O AUID- ORCID: 0000-0002-9920-7356 AD - Department of Drug Radiation Research, National Centre for Radiation Research and Technology (NCRRT) Atomic Energy Authority, Nasr City, Cairo, 11137, Egypt. omniaabdelrahman132@gmail.com. LA - eng PT - Journal Article DEP - 20200625 PL - Germany TA - Environ Sci Pollut Res Int JT - Environmental science and pollution research international JID - 9441769 RN - 0 (Antioxidants) SB - IM MH - Animals MH - Antioxidants MH - *Chemical and Drug Induced Liver Injury, Chronic MH - Humans MH - Inflammation MH - *Kefir MH - Lipid Peroxidation MH - Liver MH - Male MH - Oxidative Stress MH - Rats OTO - NOTNLM OT - Hepatotoxicity OT - Kefir OT - MCP-1 OT - NF-kappaB OT - Oxidative stress OT - gamma-irradiation EDAT- 2020/06/27 06:00 MHDA- 2020/08/29 06:00 CRDT- 2020/06/27 06:00 PHST- 2019/12/13 00:00 [received] PHST- 2020/06/22 00:00 [accepted] PHST- 2020/06/27 06:00 [pubmed] PHST- 2020/08/29 06:00 [medline] PHST- 2020/06/27 06:00 [entrez] AID - 10.1007/s11356-020-09833-7 [pii] AID - 10.1007/s11356-020-09833-7 [doi] PST - ppublish SO - Environ Sci Pollut Res Int. 2020 Oct;27(28):35161-35173. doi: 10.1007/s11356-020-09833-7. Epub 2020 Jun 25.