PMID- 32588489
OWN - NLM
STAT- MEDLINE
DCOM- 20210714
LR  - 20210714
IS  - 1098-2825 (Electronic)
IS  - 0887-8013 (Print)
IS  - 0887-8013 (Linking)
VI  - 34
IP  - 9
DP  - 2020 Sep
TI  - Comparison between paramagnetic and CD71 magnetic activated cell sorting of fetal 
      nucleated red blood cells from the maternal blood.
PG  - e23420
LID - 10.1002/jcla.23420 [doi]
LID - e23420
AB  - BACKGROUND: Fetal nucleated red blood cells (NRBC) from maternal circulation are 
      rare events but can be enriched and used to evaluate the genetics of the fetus. 
      We compared two simplified selection methods of the fetal cells from the maternal 
      blood. METHODS: We isolated fetal cells from maternal blood through 
      double-density gradient centrifugation followed either by magnetic cell 
      selection, based on the paramagnetic proprieties of the NRBC hemoglobin, 
      converted to methemoglobin, or by a positive magnetic-activated cell sorting 
      (MACS) enrichment, using anti-CD71 monoclonal antibodies. Finally, the cells were 
      identified through fluorescence in situ hybridization (FISH) with specific 
      chromosome X and Y probes. RESULTS: We processed 10 mL of peripheral blood 
      samples from 27 pregnant women with singleton normal male fetuses. 
      Hemoglobin-based enrichment isolated significantly more NRBCs: 29.7 x 10(4) cells 
      than anti-CD71 MACS: 10.1 x 10(4) cells (P < .001). The FISH analysis found at 
      least one XY cell in 81.5% and 61.5% of cases, respectively, for paramagnetic and 
      anti-CD71 selection. Also, the average number of XY cells identified through 
      paramagnetic selection was 5.09 +/- 2.5, significantly higher than those observed 
      through CD71 sorting: 3.38 +/- 1.7 cells (average +/- SE) (P = .03). CONCLUSION: The 
      combination of density gradient centrifugation with paramagnetic selection has 
      the advantage of simplicity and achieves a minimal manipulation and treatment of 
      cells. It yields an increased number of NRBCs and FISH confirmed fetal cells, 
      compared to the anti-CD71 sorting.
CI  - (c) 2020 The Authors. Journal of Clinical Laboratory Analysis published by Wiley 
      Periodicals LLC.
FAU - Nemescu, Dragos
AU  - Nemescu D
AUID- ORCID: 0000-0003-2345-1077
AD  - Grigore T Popa University of Medicine and Pharmacy, Iasi, Romania.
FAU - Constantinescu, Daniela
AU  - Constantinescu D
AUID- ORCID: 0000-0003-0732-3745
AD  - Grigore T Popa University of Medicine and Pharmacy, Iasi, Romania.
FAU - Gorduza, Vlad
AU  - Gorduza V
AUID- ORCID: 0000-0002-6776-4844
AD  - Grigore T Popa University of Medicine and Pharmacy, Iasi, Romania.
FAU - Carauleanu, Alexandru
AU  - Carauleanu A
AUID- ORCID: 0000-0001-9112-7039
AD  - Grigore T Popa University of Medicine and Pharmacy, Iasi, Romania.
FAU - Caba, Lavinia
AU  - Caba L
AUID- ORCID: 0000-0001-6327-4461
AD  - Grigore T Popa University of Medicine and Pharmacy, Iasi, Romania.
FAU - Navolan, Dan Bogdan
AU  - Navolan DB
AUID- ORCID: 0000-0002-3263-1769
AD  - Victor Babes University of Medicine and Pharmacy, Timisoara, Romania.
LA  - eng
GR  - 1196/National Program for Research Development and Innovation II/
PT  - Comparative Study
PT  - Journal Article
DEP - 20200625
PL  - United States
TA  - J Clin Lab Anal
JT  - Journal of clinical laboratory analysis
JID - 8801384
RN  - 0 (Antigens, CD)
RN  - 0 (CD71 antigen)
RN  - 0 (Receptors, Transferrin)
SB  - IM
MH  - Adult
MH  - Antigens, CD/*metabolism
MH  - Cell Separation/*methods
MH  - Erythroblasts/metabolism/*pathology
MH  - Female
MH  - Fetal Blood/*cytology
MH  - Fetus/*metabolism/pathology
MH  - Gestational Age
MH  - Humans
MH  - Immunomagnetic Separation/*methods
MH  - Male
MH  - Maternal-Fetal Exchange
MH  - Pregnancy
MH  - Prenatal Care
MH  - Prenatal Diagnosis/*methods
MH  - Receptors, Transferrin/*metabolism
PMC - PMC7521243
OTO - NOTNLM
OT  - CD71
OT  - fluorescent in situ hybridization
OT  - magnetic-activated cell sorting
OT  - nucleated red blood cells
OT  - prenatal diagnosis
EDAT- 2020/06/27 06:00
MHDA- 2021/07/15 06:00
PMCR- 2020/06/25
CRDT- 2020/06/27 06:00
PHST- 2020/02/22 00:00 [received]
PHST- 2020/04/29 00:00 [revised]
PHST- 2020/05/18 00:00 [accepted]
PHST- 2020/06/27 06:00 [pubmed]
PHST- 2021/07/15 06:00 [medline]
PHST- 2020/06/27 06:00 [entrez]
PHST- 2020/06/25 00:00 [pmc-release]
AID - JCLA23420 [pii]
AID - 10.1002/jcla.23420 [doi]
PST - ppublish
SO  - J Clin Lab Anal. 2020 Sep;34(9):e23420. doi: 10.1002/jcla.23420. Epub 2020 Jun 
      25.