PMID- 32593148
OWN - NLM
STAT- MEDLINE
DCOM- 20210401
LR  - 20210401
IS  - 1873-2828 (Electronic)
IS  - 1350-4177 (Linking)
VI  - 68
DP  - 2020 Nov
TI  - Ultrasound driven conformational and physicochemical changes of soy protein 
      hydrolysates.
PG  - 105202
LID - S1350-4177(20)30942-1 [pii]
LID - 10.1016/j.ultsonch.2020.105202 [doi]
AB  - The effect of ultrasound on the conformational and physicochemical properties of 
      soy protein isolate hydrolysates (SPHs) was investigated. SPHs were prepared at 
      hydrolysis times of 20 min, 60 min, and 180 min, then treated with ultrasound for 
      10 min, 20 min, and 30 min at a frequency of 20 kHz and output powers of 150 W 
      and 450 W. The structural properties and antioxidant capacities of the aqueous 
      layer of SPHs (ASPHs) after sonication were evaluated by Fourier-transform 
      infrared spectroscopy (FTIR), intrinsic fluorescence, DPPH radical scavenging 
      activity assays, and microscopy observations. Results obtained showed that 
      ultrasound treatment significantly disrupted the peptide aggregates formed during 
      protein hydrolysis. The protein solubility was significantly increased after 
      sonication (by up to 18.33%), as did the percentage of proteins with MW < 1 kDa 
      in ASPHs. The antioxidant capacity of ASPHs also increased, as measured by DPPH 
      assay. FTIR analysis of ASPHs indicated that the protein secondary structures 
      were different, with an increase in beta-sheet and a decrease in alpha-helix and beta-turn. 
      Furthermore, the changes in fluorescence spectra of ASPHs showed the transition 
      of protein tertiary structure with a greater exposure of Trp residues in the side 
      chains. Scanning electron microscope (SEM) and atomic force microscope (AFM) 
      observations of the morphological structure of ASPHs further confirmed the 
      significant effect of sonication on disrupting peptide aggregates. In conclusion, 
      ultrasound can be used as an efficient treatment to promote the solubility of 
      protein hydrolysates.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Tian, Ran
AU  - Tian R
AD  - College of Food Science, Northeast Agricultural University, China.
FAU - Feng, Junran
AU  - Feng J
AD  - College of Food Science, Northeast Agricultural University, China.
FAU - Huang, Guo
AU  - Huang G
AD  - College of Food Science, Northeast Agricultural University, China.
FAU - Tian, Bo
AU  - Tian B
AD  - College of Food Science, Northeast Agricultural University, China.
FAU - Zhang, Yan
AU  - Zhang Y
AD  - College of Horticulture and Landscape Architecture, Northeast Agricultural 
      University, Harbin 150030, China.
FAU - Jiang, Lianzhou
AU  - Jiang L
AD  - College of Food Science, Northeast Agricultural University, China; National 
      Research Center of Soybean Engineering and Technology, Harbin 150030, China.
FAU - Sui, Xiaonan
AU  - Sui X
AD  - College of Food Science, Northeast Agricultural University, China; National 
      Research Center of Soybean Engineering and Technology, Harbin 150030, China. 
      Electronic address: xiaonan.sui@neau.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20200618
PL  - Netherlands
TA  - Ultrason Sonochem
JT  - Ultrasonics sonochemistry
JID - 9433356
RN  - 0 (Biphenyl Compounds)
RN  - 0 (Free Radical Scavengers)
RN  - 0 (Picrates)
RN  - 0 (Soybean Proteins)
RN  - DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl)
SB  - IM
MH  - Biphenyl Compounds/chemistry
MH  - *Chemical Phenomena
MH  - Free Radical Scavengers/chemistry
MH  - Hydrolysis
MH  - Molecular Weight
MH  - Particle Size
MH  - Picrates/chemistry
MH  - Protein Conformation
MH  - Solubility
MH  - Soybean Proteins/*chemistry
MH  - *Ultrasonic Waves
OTO - NOTNLM
OT  - Antioxidant capacity
OT  - Morphological structure
OT  - Protein solubility
OT  - Secondary and tertiary structure
OT  - Soy protein hydrolysate
OT  - Ultrasound treatment
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2020/06/28 06:00
MHDA- 2021/04/02 06:00
CRDT- 2020/06/28 06:00
PHST- 2020/05/16 00:00 [received]
PHST- 2020/05/24 00:00 [revised]
PHST- 2020/05/30 00:00 [accepted]
PHST- 2020/06/28 06:00 [pubmed]
PHST- 2021/04/02 06:00 [medline]
PHST- 2020/06/28 06:00 [entrez]
AID - S1350-4177(20)30942-1 [pii]
AID - 10.1016/j.ultsonch.2020.105202 [doi]
PST - ppublish
SO  - Ultrason Sonochem. 2020 Nov;68:105202. doi: 10.1016/j.ultsonch.2020.105202. Epub 
      2020 Jun 18.