PMID- 32594320 OWN - NLM STAT- MEDLINE DCOM- 20200814 LR - 20200814 IS - 1432-8798 (Electronic) IS - 0304-8608 (Linking) VI - 165 IP - 9 DP - 2020 Sep TI - Porcine reproductive and respiratory syndrome virus: phylogenetic analysis of circulating strains in the Republic of Ireland from 2016 to 2017. PG - 2057-2063 LID - 10.1007/s00705-020-04710-7 [doi] AB - In order to investigate the genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV) strains currently circulating in the Republic of Ireland (ROI), the ORF5 gene from 17 field strains originating from four vaccinating commercial herds was sequenced and phylogenetically analysed. High genetic variability was observed between farms at the nucleotide (86.3-95.2%) and amino acid (85.5-96%) levels. Phylogenetic analysis confirmed that all field strains belonged to the European species (type 1) and clustered into three separate groups within the subtype 1 subgroup. This variation may pose challenges for diagnosis and prophylactic control of PRRSV through vaccination in the ROI. FAU - Fitzgerald, Rose M AU - Fitzgerald RM AUID- ORCID: 0000-0002-0847-1830 AD - Bio-Explore, Department of Biological Sciences, Cork Institute of Technology, Rossa Avenue, Bishopstown, Cork, T12 P928, Republic of Ireland. rose.fitzgerald@mycit.ie. FAU - Collins, Patrick J AU - Collins PJ AD - Veterinary Science Division, Agri-Food and Biosciences Institute, Stormont, Belfast, BT4 3SD, Northern Ireland. FAU - McMenamey, Michael J AU - McMenamey MJ AD - Veterinary Science Division, Agri-Food and Biosciences Institute, Stormont, Belfast, BT4 3SD, Northern Ireland. FAU - Leonard, Finola C AU - Leonard FC AD - School of Veterinary Medicine, University College Dublin, Belfield, Dublin 4, D04 W6F6, Republic of Ireland. FAU - McGlynn, Hugh AU - McGlynn H AD - Bio-Explore, Department of Biological Sciences, Cork Institute of Technology, Rossa Avenue, Bishopstown, Cork, T12 P928, Republic of Ireland. FAU - O'Shea, Helen AU - O'Shea H AD - Bio-Explore, Department of Biological Sciences, Cork Institute of Technology, Rossa Avenue, Bishopstown, Cork, T12 P928, Republic of Ireland. LA - eng GR - 14/S/832 (PathSurvPig)/Department of Agriculture, Food and the Marine/ PT - Journal Article DEP - 20200627 PL - Austria TA - Arch Virol JT - Archives of virology JID - 7506870 RN - 0 (Viral Envelope Proteins) SB - IM MH - Amino Acid Sequence MH - Animals MH - Genetic Variation MH - Genotype MH - Ireland/epidemiology MH - Open Reading Frames MH - *Phylogeny MH - Porcine Reproductive and Respiratory Syndrome/epidemiology/*virology MH - Porcine respiratory and reproductive syndrome virus/*classification/genetics/*isolation & purification MH - Swine MH - Viral Envelope Proteins/genetics EDAT- 2020/07/01 06:00 MHDA- 2020/08/15 06:00 CRDT- 2020/06/29 06:00 PHST- 2020/04/21 00:00 [received] PHST- 2020/05/22 00:00 [accepted] PHST- 2020/07/01 06:00 [pubmed] PHST- 2020/08/15 06:00 [medline] PHST- 2020/06/29 06:00 [entrez] AID - 10.1007/s00705-020-04710-7 [pii] AID - 10.1007/s00705-020-04710-7 [doi] PST - ppublish SO - Arch Virol. 2020 Sep;165(9):2057-2063. doi: 10.1007/s00705-020-04710-7. Epub 2020 Jun 27.