PMID- 32594339
OWN - NLM
STAT- MEDLINE
DCOM- 20210428
LR  - 20210428
IS  - 1573-4919 (Electronic)
IS  - 0300-8177 (Linking)
VI  - 473
IP  - 1-2
DP  - 2020 Oct
TI  - Inflammation suppression by dexamethasone via inhibition of CD147-mediated NF-kappaB 
      pathway in collagen-induced arthritis rats.
PG  - 63-76
LID - 10.1007/s11010-020-03808-5 [doi]
AB  - Dexamethasone (Dex) exhibits broad-spectrum anti-inflammatory effects in chronic 
      destructive rheumatoid arthritis. We present in vivo and in vitro evidence 
      supporting the preventive effects and underlying mechanisms of Dex on 
      collagen-induced arthritis (CIA)-induced synovial injuries. After successful 
      induction of CIA, Wistar rats were administered Dex intraperitoneally (1 mg/kg) 
      three times a week for more than 2 weeks. In vivo, paw swelling, arthritis 
      scores, and histological evaluations were analyzed to determine the therapeutic 
      effects of Dex on the progression of arthritis. In vitro, CIA fibroblast-like 
      synoviocytes (FLSs) were treated for 48 h with vehicle (control group), 10 ng/mL 
      IL-1alpha (IL-1alpha group), 10 ng/mL IL-1alpha + 10 muM Dex (Dex group), or 10 ng/mL 
      IL-1alpha + 10 mug/mL anti-CD147 antibody (anti-CD147 group). Evaluations of FLSs 
      proliferation, cell cycle, migration, gene expression of Cyclin D1, p27, p57, 
      interleukin (IL)-1beta, IL-6, IL-17, tumor necrosis factor (TNF)-alpha, CD147, CypB, 
      matrix metalloproteinase-3 (MMP-3), MMP-9, and MMP-13, ROS generation, protein 
      expression of NF-kappaB and CD147, and translocation of NF-kappaB p65 were all conducted. 
      The in vivo results showed that arthritis intensity was attenuated in the 
      Dex-treated group. The in vitro findings demonstrated that treatment with Dex 
      induced G0/G1 arrest and suppressed proliferation, migration, gene expression of 
      IL-1beta, IL-6, IL-17, TNF-alpha, CD147, CypB, MMP-3, MMP-9, and MMP-13, ROS generation 
      and protein expression of NF-kappaB and CD147. Translocation of NF-kappaB p65 was 
      inhibited by both Dex and anti-CD147 monoclonal antibody treatment. We offer 
      molecular evidence of the anti-rheumatism efficacy Dex through hindrance to 
      CD147, splendidly stabilization of the oxidative stress by downregulating the 
      NF-kappaB signaling pathway.
FAU - Wang, Qishan
AU  - Wang Q
AD  - Departments of Pharmacy, Shanghai Ninth People's Hospital, Shanghai Jiao Tong 
      University School of Medicine, Shanghai, People's Republic of China.
FAU - Xu, Bingxin
AU  - Xu B
AD  - Departments of Pharmacy, Shanghai Ninth People's Hospital, Shanghai Jiao Tong 
      University School of Medicine, Shanghai, People's Republic of China.
FAU - Fan, Kaijian
AU  - Fan K
AD  - Departments of Pharmacy, Shanghai Ninth People's Hospital, Shanghai Jiao Tong 
      University School of Medicine, Shanghai, People's Republic of China.
FAU - Wu, Jing
AU  - Wu J
AD  - Departments of Pharmacy, Shanghai Ninth People's Hospital, Shanghai Jiao Tong 
      University School of Medicine, Shanghai, People's Republic of China.
FAU - Wang, Tingyu
AU  - Wang T
AUID- ORCID: 0000-0001-5000-7890
AD  - Departments of Pharmacy, Shanghai Ninth People's Hospital, Shanghai Jiao Tong 
      University School of Medicine, Shanghai, People's Republic of China. 
      drtywang@163.com.
LA  - eng
GR  - 81874011/National Natural Science Foundation of China/
GR  - 81572104/National Natural Science Foundation of China/
GR  - 81301531/National Natural Science Foundation of China/
GR  - 18140903502/Science and Technology Commission of Shanghai Municipality/
PT  - Journal Article
DEP - 20200627
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - 0 (Cytokines)
RN  - 0 (Rela protein, rat)
RN  - 0 (Transcription Factor RelA)
RN  - 136894-56-9 (Basigin)
RN  - 7S5I7G3JQL (Dexamethasone)
SB  - IM
MH  - Animals
MH  - Arthritis, Experimental/drug therapy/*metabolism/pathology
MH  - Basigin/*metabolism
MH  - Cytokines/biosynthesis
MH  - Dexamethasone/*pharmacology
MH  - Gene Expression Regulation/*drug effects
MH  - Male
MH  - Rats
MH  - Rats, Wistar
MH  - Signal Transduction/*drug effects
MH  - Transcription Factor RelA/*metabolism
OTO - NOTNLM
OT  - Arthritis
OT  - CD147
OT  - Dexamethasone
OT  - Inflammation
OT  - NF-kappab
OT  - ROS
EDAT- 2020/07/01 06:00
MHDA- 2021/04/29 06:00
CRDT- 2020/06/29 06:00
PHST- 2020/04/14 00:00 [received]
PHST- 2020/06/18 00:00 [accepted]
PHST- 2020/07/01 06:00 [pubmed]
PHST- 2021/04/29 06:00 [medline]
PHST- 2020/06/29 06:00 [entrez]
AID - 10.1007/s11010-020-03808-5 [pii]
AID - 10.1007/s11010-020-03808-5 [doi]
PST - ppublish
SO  - Mol Cell Biochem. 2020 Oct;473(1-2):63-76. doi: 10.1007/s11010-020-03808-5. Epub 
      2020 Jun 27.