PMID- 32595184
OWN - NLM
STAT- MEDLINE
DCOM- 20210325
LR  - 20210325
IS  - 1347-4405 (Electronic)
IS  - 1342-6311 (Print)
IS  - 1342-6311 (Linking)
VI  - 35
IP  - 3
DP  - 2020
TI  - EDTA-FISH: A Simple and Effective Approach to Reduce Non-specific Adsorption of 
      Probes in Fluorescence in situ Hybridization (FISH) for Environmental Samples.
LID - 10.1264/jsme2.ME20062 [doi]
LID - ME20062
AB  - Fluorescence in situ hybridization (FISH) is a widely used molecular technique in 
      microbial ecology. However, the non-specific adsorption of fluorescent probes and 
      resulting high intensity of background signals from mineral particles hampers the 
      specific detection of microbial cells in grain-rich environmental samples, such 
      as subseafloor sediments. We herein demonstrated that a new buffer composition 
      containing EDTA efficiently reduced the adsorption of probes without compromising 
      the properties of the FISH-based probing of microbes. The inclusion of a high 
      concentration of EDTA in the buffer in our protocol provides a simple and 
      effective approach for reducing the background in FISH for environmental samples.
FAU - Morono, Yuki
AU  - Morono Y
AD  - Geomicrobiology Group, Kochi Institute for Core Sample Research, Japan Agency for 
      Earth-Marine Science and Technology (JAMSTEC).
FAU - Kubota, Kengo
AU  - Kubota K
AD  - Department of Civil and Environmental Engineering, Tohoku University.
FAU - Tsukagoshi, Daisuke
AU  - Tsukagoshi D
AD  - Department of Civil and Environmental Engineering, Tohoku University.
FAU - Terada, Takeshi
AU  - Terada T
AD  - Marine Works Japan LTD.
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Microbes Environ
JT  - Microbes and environments
JID - 9710937
RN  - 0 (Indicators and Reagents)
RN  - 0 (Oligonucleotide Probes)
RN  - 9G34HU7RV0 (Edetic Acid)
SB  - IM
MH  - Adsorption
MH  - Edetic Acid/*chemistry
MH  - Fluorescence
MH  - Geologic Sediments/microbiology
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Indicators and Reagents/chemistry
MH  - Oligonucleotide Probes/*chemistry
MH  - Seawater/microbiology
PMC - PMC7511785
OTO - NOTNLM
OT  - EDTA
OT  - Fluorescence in situ hybridization (FISH)
OT  - marine sediments
EDAT- 2020/07/01 06:00
MHDA- 2021/03/26 06:00
PMCR- 2020/01/01
CRDT- 2020/06/30 06:00
PHST- 2020/06/30 06:00 [entrez]
PHST- 2020/07/01 06:00 [pubmed]
PHST- 2021/03/26 06:00 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - DN/JST.JSTAGE/jsme2/ME20062 [pii]
AID - 10.1264/jsme2.ME20062 [doi]
PST - ppublish
SO  - Microbes Environ. 2020;35(3):ME20062. doi: 10.1264/jsme2.ME20062.