PMID- 32597300
OWN - NLM
STAT- MEDLINE
DCOM- 20210902
LR  - 20211204
IS  - 1551-4005 (Electronic)
IS  - 1538-4101 (Print)
IS  - 1551-4005 (Linking)
VI  - 19
IP  - 15
DP  - 2020 Aug
TI  - Human umbilical cord mesenchymal stem cell-derived exosome-mediated transfer of 
      microRNA-133b boosts trophoblast cell proliferation, migration and invasion in 
      preeclampsia by restricting SGK1.
PG  - 1869-1883
LID - 10.1080/15384101.2020.1769394 [doi]
AB  - OBJECTIVE: Exosomes have been documented to function in human diseases, yet their 
      transfer of microRNA (miRNA) in preeclampsia (PE) has seldom been reported. This 
      study intends to discuss the role of miR-133b derived from exosomes in human 
      umbilical cord mesenchymal stem cells (hUC-MSCs) in trophoblast cell development 
      in PE. METHODS: Placentas from PE patients and normal pregnant women were 
      collected. The hUC-MSCs and their exosomes were obtained and identified. 
      Trophoblast cell HPT-8 and HTR8-S/Vneo were obtained and co-cultured with 
      hUC-MSCs-derived exosomes that had been transfected with different miR-133b 
      plasmids. MiR-133b and glucocorticoid-regulated kinase 1 (SGK1) expression in 
      placental tissues and HPT-8 and HTR8-S/Vneo cells was determined. HTR8-S/Vneo and 
      HPT-8 cell proliferation, cell cycle distribution, apoptosis rate, migration and 
      invasion were detected. RESULTS: MiR-133b was down-regulated and SGK1 was 
      up-regulated in placental tissues of PE patients. MiR-133b expression was 
      inversely related to SGK1 expression in HTR8-S/Vneo and HPT-8 cells co-cultured 
      with hUC-MSC-derived exosomes. Exosomes promoted HTR8-S/Vneo and HPT-8 cell 
      proliferation, migration and invasion abilities, cell cycle entry and inhibited 
      apoptosis. Elevated exosome-derived miR-133b from hUC-MSCs boosted HTR8-S/Vneo 
      and HPT-8 cell proliferation, cell cycle progression, migration and invasion and 
      limited cell apoptosis. MiR-133b targeted SGK1. CONCLUSION: Collectively, we 
      demonstrate that miR-133b is down-regulated and SGK1 is up-regulated in PE, and 
      miR-133b derived from exosomes in hUM-MSCs facilitates trophoblast cell 
      proliferation, migration and invasion in PE via constraining SGK1.
FAU - Wang, Dan
AU  - Wang D
AD  - Department of Obstetric and Gynecology, Shengjing Hospital of China Medical 
      University , Shengyang, Liaoning, China.
FAU - Na, Quan
AU  - Na Q
AD  - Department of Obstetric and Gynecology, Shengjing Hospital of China Medical 
      University , Shengyang, Liaoning, China.
FAU - Song, Gui Yu
AU  - Song GY
AD  - Department of Obstetric and Gynecology, Shengjing Hospital of China Medical 
      University , Shengyang, Liaoning, China.
FAU - Wang, Leilei
AU  - Wang L
AD  - Department of Obstetric and Gynecology, Shengjing Hospital of China Medical 
      University , Shengyang, Liaoning, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200628
PL  - United States
TA  - Cell Cycle
JT  - Cell cycle (Georgetown, Tex.)
JID - 101137841
RN  - 0 (Immediate-Early Proteins)
RN  - 0 (MIRN133 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (serum-glucocorticoid regulated kinase)
SB  - IM
MH  - Adult
MH  - Apoptosis
MH  - Base Sequence
MH  - Cell Cycle
MH  - *Cell Movement
MH  - Cell Proliferation
MH  - Exosomes/*metabolism
MH  - Female
MH  - Humans
MH  - Immediate-Early Proteins/*metabolism
MH  - Mesenchymal Stem Cells/*metabolism
MH  - MicroRNAs/genetics/*metabolism
MH  - Pre-Eclampsia/*pathology
MH  - Pregnancy
MH  - Protein Serine-Threonine Kinases/*metabolism
MH  - Trophoblasts/*pathology
MH  - Umbilical Cord/*cytology
PMC - PMC7469539
OTO - NOTNLM
OT  - Preeclampsia
OT  - SGK1
OT  - exosomes
OT  - human umbilical cord mesenchymal stem cells
OT  - microRNA-133b
OT  - trophoblast cells
COIS- The authors declare that they have no conflicts of interest.
EDAT- 2020/07/01 06:00
MHDA- 2021/09/03 06:00
PMCR- 2021/06/28
CRDT- 2020/06/30 06:00
PHST- 2020/07/01 06:00 [pubmed]
PHST- 2021/09/03 06:00 [medline]
PHST- 2020/06/30 06:00 [entrez]
PHST- 2021/06/28 00:00 [pmc-release]
AID - 1769394 [pii]
AID - 10.1080/15384101.2020.1769394 [doi]
PST - ppublish
SO  - Cell Cycle. 2020 Aug;19(15):1869-1883. doi: 10.1080/15384101.2020.1769394. Epub 
      2020 Jun 28.