PMID- 32597748 OWN - NLM STAT- MEDLINE DCOM- 20200904 LR - 20231214 IS - 1473-5644 (Electronic) IS - 0022-2615 (Print) IS - 0022-2615 (Linking) VI - 69 IP - 8 DP - 2020 Aug TI - Matrix-assisted laser desorption/ionization time-of-flight MS for the accurate identification of Burkholderia cepacia complex and Burkholderia gladioli in the clinical microbiology laboratory. PG - 1105-1113 LID - 10.1099/jmm.0.001223 [doi] AB - Introduction. Burkholderia cepacia complex (Bcc) bacteria, currently consisting of 23 closely related species, and Burkholderia gladioli, can cause serious and difficult-to-treat infections in people with cystic fibrosis. Identifying Burkholderia bacteria to the species level is considered important for understanding epidemiology and infection control, and predicting clinical outcomes. Matrix-assisted laser desorption/ionization time-of-flight MS (MALDI-TOF) is a rapid method recently introduced in clinical laboratories for bacterial species-level identification. However, reports on the ability of MALDI-TOF to accurately identify Bcc to the species level are mixed.Aim. The aim of this project was to evaluate the accuracy of MALDI-TOF using the Biotyper and VITEK MS systems in identifying isolates from 22 different Bcc species and B. gladioli compared to recA gene sequencing, which is considered the current gold standard for Bcc.Methodology. To capture maximum intra-species variation, phylogenetic trees were constructed from concatenated multi-locus sequence typing alleles and clustered with a novel k-medoids approach. One hundred isolates representing 22 Bcc species, plus B. gladioli, were assessed for bacterial identifications using the two MALDI-TOF systems.Results. At the genus level, 100 and 97.0 % of isolates were confidently identified as Burkholderia by the Biotyper and VITEK MS systems, respectively; moreover, 26.0 and 67.0 % of the isolates were correctly identified to the species level, respectively. In many, but not all, cases of species misidentification or failed identification, a representative library for that species was lacking.Conclusion. Currently available MALDI-TOF systems frequently do not accurately identify Bcc bacteria to the species level. FAU - Wong, Kendrew S K AU - Wong KSK AD - Division of Respiratory Medicine, Department of Pediatrics, Faculty of Medicine, University of British Columbia, Vancouver, BC, Canada. FAU - Dhaliwal, Suk AU - Dhaliwal S AD - Microbiology, BC Children's Hospital, Vancouver, BC, Canada. FAU - Bilawka, Jennifer AU - Bilawka J AD - Pathology and Laboratory Medicine, Providence Health Care, Vancouver, BC, Canada. FAU - Srigley, Jocelyn A AU - Srigley JA AD - Department of Pathology and Laboratory Medicine, Faculty of Medicine, University of British Columbia, Vancouver, BC, Canada. FAU - Champagne, Sylvie AU - Champagne S AD - Pathology and Laboratory Medicine, Providence Health Care, Vancouver, BC, Canada. AD - Department of Pathology and Laboratory Medicine, Faculty of Medicine, University of British Columbia, Vancouver, BC, Canada. FAU - Romney, Marc G AU - Romney MG AD - Pathology and Laboratory Medicine, Providence Health Care, Vancouver, BC, Canada. AD - Department of Pathology and Laboratory Medicine, Faculty of Medicine, University of British Columbia, Vancouver, BC, Canada. FAU - Tilley, Peter AU - Tilley P AD - Department of Pathology and Laboratory Medicine, Faculty of Medicine, University of British Columbia, Vancouver, BC, Canada. FAU - Sadarangani, Manish AU - Sadarangani M AD - Division of Infectious Diseases, Department of Pediatrics, Faculty of Medicine, University of British Columbia, Vancouver, BC, Canada. AD - Vaccine Evaluation Centre, BC Children's Hospital, Vancouver, BC, Canada. FAU - Zlosnik, James E A AU - Zlosnik JEA AD - Division of Infectious Diseases, Department of Pediatrics, Faculty of Medicine, University of British Columbia, Vancouver, BC, Canada. FAU - Chilvers, Mark A AU - Chilvers MA AD - Division of Respiratory Medicine, Department of Pediatrics, Faculty of Medicine, University of British Columbia, Vancouver, BC, Canada. LA - eng PT - Comparative Study PT - Journal Article DEP - 20200629 PL - England TA - J Med Microbiol JT - Journal of medical microbiology JID - 0224131 RN - 0 (DNA, Bacterial) RN - EC 2.7.7.- (Rec A Recombinases) SB - IM MH - Animals MH - Bacterial Typing Techniques/methods MH - Burkholderia cepacia/classification/*isolation & purification MH - Burkholderia gladioli/classification/*isolation & purification MH - Cluster Analysis MH - DNA, Bacterial/chemistry/isolation & purification MH - Fourier Analysis MH - Humans MH - Multilocus Sequence Typing MH - Phylogeny MH - Rec A Recombinases/genetics MH - Sequence Alignment MH - *Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization PMC - PMC7642978 OTO - NOTNLM OT - Bacterial identification OT - Burkholderia cepacia complex OT - Burkholderia gladioli OT - MALDI-TOF OT - cystic fibrosis COIS- M.R.G. has served as a consultant to bioMerieux Canada. M.S. is supported via salary awards from the BC Children's Hospital Foundation, the Canadian Child Health Clinician Scientist Program and the Michael Smith Foundation for Health Research. M.S. has been an investigator on projects funded by Pfizer, Merck, Seqirus, Sanofi-Pasteur, VBI Vaccines and GlaxoSmithKline. All funds have been paid to his institute, and he has not received any personal payments. J.E.A.Z. is supported by a grant from Cystic Fibrosis Canada to M.S., J.E.A.Z. and M.C. to operate the Canadian Burkholderia cepacia complex research and referral repository. EDAT- 2020/07/01 06:00 MHDA- 2020/09/05 06:00 PMCR- 2020/06/29 CRDT- 2020/06/30 06:00 PHST- 2020/07/01 06:00 [pubmed] PHST- 2020/09/05 06:00 [medline] PHST- 2020/06/30 06:00 [entrez] PHST- 2020/06/29 00:00 [pmc-release] AID - 001223 [pii] AID - 10.1099/jmm.0.001223 [doi] PST - ppublish SO - J Med Microbiol. 2020 Aug;69(8):1105-1113. doi: 10.1099/jmm.0.001223. Epub 2020 Jun 29.