PMID- 32602772
OWN - NLM
STAT- MEDLINE
DCOM- 20200930
LR  - 20200930
IS  - 1532-4109 (Electronic)
IS  - 0360-1234 (Linking)
VI  - 55
IP  - 9
DP  - 2020
TI  - Chlorpyrifos-induced toxicity has no gender selectivity in the early fetal brain.
PG  - 803-812
LID - 10.1080/03601234.2020.1786326 [doi]
AB  - Organophosphorus pesticides induce gender-specific developmental neurotoxicity 
      after birth, especially in adolescents and adults. However, whether and when the 
      selectivity occurs in fetus remains unclear. In this study, we analyzed 
      chlorpyrifos (CPF)-induced neurotoxicity in the early fetal brains of male and 
      female mice. The gestational dams were administered 0, 1, 3, and 5 mg/(kg.d) CPF 
      during gestational days (GD)7-11, and brains from the fetuses were isolated and 
      analyzed on GD12. Fetal gender was identified by PCR technique based on 
      male-specific Sry gene and Myog control gene. The body weight and head weight, 
      the activity of acetylcholinesterase (AChE), superoxide dismutase (SOD), catalase 
      (CAT), glutathione peroxidase (GPX), and the content of malondialdehyde (MDA), as 
      well as the oxidative stress-related gene expression were examined. Our results 
      showed that CPF pretreatment induced AChE inhibition in GD12 fetal brain. CPF 
      treatment activated SOD and GPX but not CAT and MDA. For oxidative stress-related 
      gene expression, CPF pretreatment increased mRNA expression of Sod1, Cat, Gpx1, 
      and Gpx2 in the fetal brain on GD12. The statistical analysis did not show 
      gender-selective CPF-induced toxicity. Moreover, our results showed that although 
      the gestational exposure to CPF could elicit abnormalities in the early fetal 
      brain, the toxicity observed was not gender-specific.
FAU - Gu, Jiabin
AU  - Gu J
AD  - College of Biotechnology and Bioengineering, Zhejiang University of Technology, 
      Hangzhou, China.
FAU - Xu, Shuai
AU  - Xu S
AD  - College of Biotechnology and Bioengineering, Zhejiang University of Technology, 
      Hangzhou, China.
FAU - Liu, Yuqiong
AU  - Liu Y
AD  - College of Biotechnology and Bioengineering, Zhejiang University of Technology, 
      Hangzhou, China.
FAU - Chen, Xiaoping
AU  - Chen X
AD  - College of Biotechnology and Bioengineering, Zhejiang University of Technology, 
      Hangzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20200630
PL  - England
TA  - J Environ Sci Health B
JT  - Journal of environmental science and health. Part. B, Pesticides, food 
      contaminants, and agricultural wastes
JID - 7607167
RN  - 0 (Cholinesterase Inhibitors)
RN  - 0 (Insecticides)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 3.1.1.7 (Acetylcholinesterase)
RN  - JCS58I644W (Chlorpyrifos)
SB  - IM
MH  - Acetylcholinesterase/metabolism
MH  - Animals
MH  - Body Weight/drug effects
MH  - Brain/*drug effects/*embryology/metabolism
MH  - Catalase/genetics/metabolism
MH  - Chlorpyrifos/*toxicity
MH  - Cholinesterase Inhibitors/toxicity
MH  - Female
MH  - Gene Expression Regulation, Developmental/drug effects
MH  - Insecticides/*toxicity
MH  - Male
MH  - Malondialdehyde/metabolism
MH  - Mice, Inbred ICR
MH  - Neurotoxicity Syndromes/etiology
MH  - Organ Size/drug effects
MH  - Oxidative Stress/drug effects/genetics
MH  - Sex Factors
MH  - Superoxide Dismutase/genetics/metabolism
MH  - Toxicity Tests/methods
OTO - NOTNLM
OT  - Organophosphorus pesticides
OT  - developmental neurotoxicity
OT  - early neurogenesis
OT  - gender selectivity
EDAT- 2020/07/01 06:00
MHDA- 2020/10/02 06:00
CRDT- 2020/07/01 06:00
PHST- 2020/07/01 06:00 [pubmed]
PHST- 2020/10/02 06:00 [medline]
PHST- 2020/07/01 06:00 [entrez]
AID - 10.1080/03601234.2020.1786326 [doi]
PST - ppublish
SO  - J Environ Sci Health B. 2020;55(9):803-812. doi: 10.1080/03601234.2020.1786326. 
      Epub 2020 Jun 30.