PMID- 32603879 OWN - NLM STAT- MEDLINE DCOM- 20201124 LR - 20231213 IS - 1879-1506 (Electronic) IS - 0003-9969 (Linking) VI - 117 DP - 2020 Sep TI - Botulinum toxin A alleviates orofacial nociception induced by orthodontic tooth movement through nociceptin/orphanin-FQ pathway in rats. PG - 104817 LID - S0003-9969(20)30195-3 [pii] LID - 10.1016/j.archoralbio.2020.104817 [doi] AB - OBJECTIVES: To investigate the effect and mechanism of botulinum neurotoxin type A (BoNT/A) in the modulation of orofacial nociception induced by orthodontic tooth movement in rats. METHODS: An orofacial nociception model was established in male Sprague-Dawley rats by ligating closed-coil springs between incisors and ipsilateral molars. There were two group sets of animals. For the first group set, 120 rats were randomly divided into four groups: no-force group (n = 30), force + saline group (n = 30), force + low dose BoNT/A group (1U/6 muL, n = 30), and force + high dose BoNT/A group (1U/6 muL, n = 30). BoNT/A and saline were injected into periodontal ligament to explore the nociceptive effect of BoNT/A. Ipsilateral trigeminal ganglia (TG) were harvested for detecting the expression levels of nociceptin/orphanin-FQ (N/OFQ). For the second group set, 36 rats were randomly divided into three force groups: BoNT/A + saline group (n = 12), BoNT/A + UFP-101 group (n = 12), and saline + UFP-101 group (n = 12). A potent N/OFQ receptor (NOP) antagonist (UFP-101) was used to examine the role of N/OFQ in BoNT/A-induced antinociception. Tooth-movement nociception level of all groups was evaluated by bite force and rat grimace scale (RGS) at baseline, day 1, day 3, day 5, day 7, day 14. RESULTS: The behavioral assessments showed the orofacial nociception level in the force + low dose BoNT/A group and force + high dose BoNT/A group were lower than that in the force + saline group. No significant difference was observed in orofacial nociception among no-force group, force + low dose and force + high dose group. The expression levels of N/OFQ in TG were elevated from day 1 and maintained a high level, presenting in descending order among the force + high dose, force + low dose, force + saline and no-force group, respectively. The nociception level of the BoNT/A + UFP-101 group was higher than that of the BoNT/A + saline group. No significant difference was observed between the BoNT/A + UFP-101 group and the saline + UFP-101 group. CONCLUSIONS: BoNT/A can exert an antinociceptive effect on orofacial nociception induced by tooth movement by stimulating the expression of N/OFQ in TG. CI - Copyright (c) 2020 Elsevier Ltd. All rights reserved. FAU - Lyu, Jiahong AU - Lyu J AD - Department of Orthodontics, State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China. FAU - Wen, Jing AU - Wen J AD - Department of Orthodontics, State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China. FAU - Guo, Rui AU - Guo R AD - Department of Orthodontics, State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China. FAU - Zhu, Yafen AU - Zhu Y AD - Department of Orthodontics, State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China. FAU - Liang, Hengyan AU - Liang H AD - Department of Orthodontics, State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China. FAU - Gao, Meiya AU - Gao M AD - Department of Orthodontics, State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China. FAU - Wang, Hang AU - Wang H AD - The Plastic and Cosmetic Center, State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China. FAU - Lai, Wenli AU - Lai W AD - Department of Orthodontics, State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China. FAU - Long, Hu AU - Long H AD - Department of Orthodontics, State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China. Electronic address: hlong@scu.edu.cn. LA - eng PT - Journal Article DEP - 20200612 PL - England TA - Arch Oral Biol JT - Archives of oral biology JID - 0116711 RN - 0 (Opioid Peptides) RN - 0 (Receptors, Opioid) RN - EC 3.4.24.69 (Botulinum Toxins, Type A) SB - IM MH - Animals MH - Botulinum Toxins, Type A/*therapeutic use MH - Male MH - *Nociception MH - Opioid Peptides/*metabolism MH - Rats MH - Rats, Sprague-Dawley MH - Receptors, Opioid/*metabolism MH - Tooth Movement Techniques/*adverse effects MH - Nociceptin OTO - NOTNLM OT - Antinociception OT - Botulinum toxin A OT - Nociceptin/orphanin-FQ OT - Orofacial nociception OT - Trigeminal ganglia COIS- Declaration of Competing Interest The authors declare that there is no conflict of interest. EDAT- 2020/07/01 06:00 MHDA- 2020/11/25 06:00 CRDT- 2020/07/01 06:00 PHST- 2019/11/27 00:00 [received] PHST- 2020/06/06 00:00 [revised] PHST- 2020/06/08 00:00 [accepted] PHST- 2020/07/01 06:00 [pubmed] PHST- 2020/11/25 06:00 [medline] PHST- 2020/07/01 06:00 [entrez] AID - S0003-9969(20)30195-3 [pii] AID - 10.1016/j.archoralbio.2020.104817 [doi] PST - ppublish SO - Arch Oral Biol. 2020 Sep;117:104817. doi: 10.1016/j.archoralbio.2020.104817. Epub 2020 Jun 12.