PMID- 32605184
OWN - NLM
STAT- MEDLINE
DCOM- 20210319
LR  - 20210319
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 21
IP  - 13
DP  - 2020 Jun 28
TI  - Early Elevation of Systemic Plasma Clusterin after Reperfused Acute Myocardial 
      Infarction in a Preclinical Porcine Model of Ischemic Heart Disease.
LID - 10.3390/ijms21134591 [doi]
LID - 4591
AB  - Clusterin exerts anti-inflammatory, cytoprotective and anti-apoptotic effects. 
      Both an increase and decrease of clusterin in acute myocardial infarction (AMI) 
      has been reported. We aimed to clarify the role of clusterin as a systemic 
      biomarker in AMI. AMI was induced by percutaneous left anterior artery (LAD) 
      occlusion for 90 min followed by reperfusion in 24 pigs. Contrast 
      ventriculography was performed after reperfusion to assess left ventricular 
      ejection fraction (LVEF), left ventricular end diastolic volume (LVEDV) and left 
      ventricular end systolic volume (LVESV) and additional cMRI + late enhancement to 
      measure infarct size and LV functions at day 3 and week 6 post-MI. Blood samples 
      were collected at prespecified timepoints. Plasma clusterin and other biomarkers 
      (cTnT, NT-proBNP, neprilysin, NGAL, ET-1, osteopontin, miR21, miR29) were 
      measured by ELISA and qPCR. Gene expression profiles of infarcted and remote 
      region 3 h (n = 5) and 3 days (n = 5) after AMI onset were analysed by 
      RNA-sequencing. AMI led to an increase in LVEDV and LVESV during 6-week, with 
      concomitant elevation of NT-proBNP 3-weeks after AMI. Plasma clusterin levels 
      were increased immediately after AMI and returned to normal levels until 3-weeks. 
      Plasma NGAL, ET-1 and miR29 was significantly elevated at 3 weeks follow-up, 
      miR21 increased after reperfusion and at 3 weeks post-AMI, while circulating 
      neprilysin levels did not change. Elevated plasma clusterin levels 120 min after 
      AMI onset suggest that clusterin might be an additional early biomarker of 
      myocardial ischemia.
FAU - Traxler, Denise
AU  - Traxler D
AD  - Division of Cardiology, Department of Internal Medicine II, Medical University of 
      Vienna, 1090 Vienna, Austria.
FAU - Spannbauer, Andreas
AU  - Spannbauer A
AD  - Division of Cardiology, Department of Internal Medicine II, Medical University of 
      Vienna, 1090 Vienna, Austria.
FAU - Einzinger, Patrick
AU  - Einzinger P
AD  - Institute of Information Systems Engineering, Research Unit of Information and 
      Software Engineering, Vienna University of Technology, 1040 Vienna, Austria.
FAU - Mester-Tonczar, Julia
AU  - Mester-Tonczar J
AD  - Division of Cardiology, Department of Internal Medicine II, Medical University of 
      Vienna, 1090 Vienna, Austria.
FAU - Lukovic, Dominika
AU  - Lukovic D
AD  - Division of Cardiology, Department of Internal Medicine II, Medical University of 
      Vienna, 1090 Vienna, Austria.
FAU - Winkler, Johannes
AU  - Winkler J
AD  - Division of Cardiology, Department of Internal Medicine II, Medical University of 
      Vienna, 1090 Vienna, Austria.
FAU - Zlabinger, Katrin
AU  - Zlabinger K
AD  - Division of Cardiology, Department of Internal Medicine II, Medical University of 
      Vienna, 1090 Vienna, Austria.
FAU - Gugerell, Alfred
AU  - Gugerell A
AD  - Division of Cardiology, Department of Internal Medicine II, Medical University of 
      Vienna, 1090 Vienna, Austria.
FAU - Mandic, LJubica
AU  - Mandic L
AD  - Division of Cardiology, Department of Internal Medicine II, Medical University of 
      Vienna, 1090 Vienna, Austria.
FAU - Gyongyosi, Mariann
AU  - Gyongyosi M
AD  - Division of Cardiology, Department of Internal Medicine II, Medical University of 
      Vienna, 1090 Vienna, Austria.
FAU - Pavo, Noemi
AU  - Pavo N
AD  - Division of Cardiology, Department of Internal Medicine II, Medical University of 
      Vienna, 1090 Vienna, Austria.
LA  - eng
PT  - Journal Article
DEP - 20200628
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Clusterin)
SB  - IM
MH  - Animals
MH  - Clusterin/*blood
MH  - *Disease Models, Animal
MH  - Female
MH  - Myocardial Infarction/blood/*pathology/therapy
MH  - Myocardial Ischemia/blood/*pathology/therapy
MH  - Reperfusion
MH  - Stroke Volume
MH  - Swine
MH  - Transcriptome
MH  - Ventricular Remodeling
PMC - PMC7369988
OTO - NOTNLM
OT  - NGS
OT  - acute myocardial infarction
OT  - clusterin
OT  - miRNAs
COIS- The authors declare no conflict of interest.
EDAT- 2020/07/02 06:00
MHDA- 2021/03/20 06:00
PMCR- 2020/07/01
CRDT- 2020/07/02 06:00
PHST- 2020/05/25 00:00 [received]
PHST- 2020/06/14 00:00 [revised]
PHST- 2020/06/23 00:00 [accepted]
PHST- 2020/07/02 06:00 [entrez]
PHST- 2020/07/02 06:00 [pubmed]
PHST- 2021/03/20 06:00 [medline]
PHST- 2020/07/01 00:00 [pmc-release]
AID - ijms21134591 [pii]
AID - ijms-21-04591 [pii]
AID - 10.3390/ijms21134591 [doi]
PST - epublish
SO  - Int J Mol Sci. 2020 Jun 28;21(13):4591. doi: 10.3390/ijms21134591.