PMID- 32608990
OWN - NLM
STAT- MEDLINE
DCOM- 20201023
LR  - 20201023
IS  - 1522-1563 (Electronic)
IS  - 0363-6143 (Linking)
VI  - 319
IP  - 3
DP  - 2020 Sep 1
TI  - Upregulating microRNA-874-3p inhibits CXCL12 expression to promote angiogenesis 
      and suppress inflammatory response in ischemic stroke.
PG  - C579-C588
LID - 10.1152/ajpcell.00001.2020 [doi]
AB  - Identification of specific biomarkers for ischemic stroke is necessary due to 
      their abilities to improve treatment outcomes. Many studies have demonstrated the 
      involvement of microRNAs (miRNAs) in the pathogenesis and complications of 
      ischemic stroke and patient outcomes. We found that the expression of miR-874-3p 
      was downregulated in clinical samples of ischemic stroke. Thus the present study 
      explored the potential role of miR-874-3p in ischemic stroke and related 
      mechanisms. A mouse model of ischemic stroke was constructed by middle cerebral 
      artery occlusion. The relationship among miR-874-3p, C-X-C motif chemokine ligand 
      12 (CXCL12), and the Wnt/beta-catenin pathway was explored by dual luciferase 
      reporter assay and Western blot analysis. Angiogenesis and brain tissue apoptosis 
      were evaluated by immunofluorescence staining and TUNEL staining, respectively. 
      ELISA was introduced to measure levels of inflammatory factors, including tumor 
      necrosis factor-alpha (TNF-alpha), interleukin (IL)-1, IL-6, IL-8, and IL-10 in brain 
      tissues. Primary hippocampal neuronal cells were isolated from the mouse model of 
      ischemic stroke and incubated with human umbilical vein endothelial cells 
      (HUVECs) for HUVEC tube formation. High expression of CXCL12 and low expression 
      of miR-874-3p were confirmed in ischemic stroke. In addition, miR-874-3p was 
      found to target and downregulate CXCL12, thus reducing TNF-alpha, IL-1, IL-6, and 
      IL-8 levels, but enhancing IL-10 level. Collectively, upregulating miR-874-3p 
      inhibits CXCL12 expression to promote angiogenesis and inhibit inflammation in 
      ischemic stroke mice by activating the Wnt/beta-catenin pathway, which may provide a 
      new direction of ischemic stroke treatment.
FAU - Xie, Kangling
AU  - Xie K
AD  - Department of Rehabilitation, Xiangya Hospital, Central South University, 
      Changsha, People's Republic of China.
FAU - Cai, Ying
AU  - Cai Y
AD  - Department of Rehabilitation, Xiangya Hospital, Central South University, 
      Changsha, People's Republic of China.
FAU - Yang, Pu
AU  - Yang P
AD  - Department of Vascular Surgery, Xiangya Hospital, Central South University, 
      Changsha, People's Republic of China.
FAU - Du, Feng
AU  - Du F
AD  - Guangzhou University of Chinese Medicine, Guangzhou, People's Republic of China.
AD  - Department of Cardiology, The First People's Hospital of Guiyang, Guiyang, 
      People's Republic of China.
FAU - Wu, Kai
AU  - Wu K
AD  - Department of Rehabilitation, Xiangya Hospital, Central South University, 
      Changsha, People's Republic of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200701
PL  - United States
TA  - Am J Physiol Cell Physiol
JT  - American journal of physiology. Cell physiology
JID - 100901225
RN  - 0 (Chemokine CXCL12)
RN  - 0 (Cxcl12 protein, mouse)
RN  - 0 (MIRN874 microRNA, mouse)
RN  - 0 (MicroRNAs)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Apoptosis/physiology
MH  - Brain Ischemia/genetics/metabolism
MH  - Chemokine CXCL12/*metabolism
MH  - Inflammation/*metabolism
MH  - Mice
MH  - MicroRNAs/*genetics
MH  - Neurons/metabolism
MH  - Stroke/genetics/*metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Up-Regulation
OTO - NOTNLM
OT  - C-X-C motif chemokine ligand 12
OT  - angiogenesis
OT  - inflammatory factor release
OT  - ischemic stroke
OT  - microRNA-874-3p
EDAT- 2020/07/02 06:00
MHDA- 2020/10/24 06:00
CRDT- 2020/07/02 06:00
PHST- 2020/07/02 06:00 [pubmed]
PHST- 2020/10/24 06:00 [medline]
PHST- 2020/07/02 06:00 [entrez]
AID - 10.1152/ajpcell.00001.2020 [doi]
PST - ppublish
SO  - Am J Physiol Cell Physiol. 2020 Sep 1;319(3):C579-C588. doi: 
      10.1152/ajpcell.00001.2020. Epub 2020 Jul 1.