PMID- 32612598
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 11
DP  - 2020
TI  - Microbial and Geochemical Dynamics of an Aquifer Stimulated for Microbial Induced 
      Calcite Precipitation (MICP).
PG  - 1327
LID - 10.3389/fmicb.2020.01327 [doi]
LID - 1327
AB  - Microbially induced calcite precipitation (MICP) is an alternative to existing 
      soil stabilization techniques for construction and erosion. As with any 
      biologically induced process in soils or aquifers, it is important to track 
      changes in the microbial communities that occur as a result of the treatment. Our 
      research assessed how native microbial communities developed in response to 
      injections of reactants (dilute molasses as a carbon source; urea as a source of 
      nitrogen and alkalinity) that promoted MICP in a shallow aquifer. Microbial 
      community composition (16S rRNA gene) and ureolytic potential (ureC gene copy 
      numbers) were also measured in groundwater and artificial sediment. Aquifer 
      geochemistry showed evidence of sulfate reduction, nitrification, 
      denitrification, ureolysis, and iron reduction during the treatment. The observed 
      changes in geochemistry corresponded to microbial community succession in the 
      groundwater and this matched parallel geophysical and mineralogical evidence of 
      calcite precipitation in the aquifer. We detected an increase in the number of 
      ureC genes in the microbial communities at the end of the injection period, 
      suggesting an increase in the abundance of microbes possessing this gene as 
      needed to hydrolyze urea and stimulate MICP. We identify geochemical and 
      biological markers that highlight the microbial community response that can be 
      used along with geophysical and geotechnical evidence to assess progress of MICP.
CI  - Copyright (c) 2020 Ohan, Saneiyan, Lee, Bartlow, Ntarlagiannis, Burns and Colwell.
FAU - Ohan, J A
AU  - Ohan JA
AD  - Department of Microbiology, Oregon State University, Corvallis, OR, United 
      States.
AD  - Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM, United 
      States.
FAU - Saneiyan, S
AU  - Saneiyan S
AD  - Department of Earth & Environmental Sciences, Rutgers University, Newark, NJ, 
      United States.
FAU - Lee, J
AU  - Lee J
AD  - College of Engineering, Georgia Institute of Technology, Atlanta, GA, United 
      States.
FAU - Bartlow, Andrew W
AU  - Bartlow AW
AD  - Bioscience Division, Los Alamos National Laboratory, Los Alamos, NM, United 
      States.
FAU - Ntarlagiannis, D
AU  - Ntarlagiannis D
AD  - Department of Earth & Environmental Sciences, Rutgers University, Newark, NJ, 
      United States.
FAU - Burns, S E
AU  - Burns SE
AD  - College of Engineering, Georgia Institute of Technology, Atlanta, GA, United 
      States.
FAU - Colwell, Frederick S
AU  - Colwell FS
AD  - College of Earth, Ocean, and Atmospheric Sciences, Oregon State University, 
      Corvallis, OR, United States.
LA  - eng
PT  - Journal Article
DEP - 20200616
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC7309221
OTO - NOTNLM
OT  - biocementation
OT  - biomineralization
OT  - biostimulation
OT  - microbial induced calcite precipitation (MICP)
OT  - soil stabilization
EDAT- 2020/07/03 06:00
MHDA- 2020/07/03 06:01
PMCR- 2020/06/16
CRDT- 2020/07/03 06:00
PHST- 2020/01/28 00:00 [received]
PHST- 2020/05/25 00:00 [accepted]
PHST- 2020/07/03 06:00 [entrez]
PHST- 2020/07/03 06:00 [pubmed]
PHST- 2020/07/03 06:01 [medline]
PHST- 2020/06/16 00:00 [pmc-release]
AID - 10.3389/fmicb.2020.01327 [doi]
PST - epublish
SO  - Front Microbiol. 2020 Jun 16;11:1327. doi: 10.3389/fmicb.2020.01327. eCollection 
      2020.