PMID- 32613452
OWN - NLM
STAT- MEDLINE
DCOM- 20220303
LR  - 20220303
IS  - 2629-3277 (Electronic)
IS  - 2629-3277 (Linking)
VI  - 17
IP  - 2
DP  - 2021 Apr
TI  - Exosomes Derived from Human Umbilical Cord Mesenchymal Stem Cells Accelerate 
      Cutaneous Wound Healing by Enhancing Angiogenesis through Delivering 
      Angiopoietin-2.
PG  - 305-317
LID - 10.1007/s12015-020-09992-7 [doi]
AB  - The underlying mechanisms of human umbilical cord mesenchymal stem cells 
      (hucMSCs) and their exosomes (hucMSC-Exs), which play significant roles in skin 
      wound healing, remain poorly understood. By using a rat model of deep 
      second-degree burn injury, the roles of hucMSC-Exs in angiogenesis and cutaneous 
      wound healing in vivo were investigated. We found that hucMSC-Exs accelerated 
      skin wound healing and angiogenesis, inducing a higher wound-closure rate and 
      increased expression of CD31 in vivo. We also discovered that hucMSC-Exs 
      contained angiopoietin-2 (Ang-2), and treatment with hucMSC-Exs enhanced the 
      expression of the Ang-2 protein in the wound area and human umbilical vein 
      endothelial cells (HUVECs) through exosomal-mediated Ang-2 transfer. Moreover, 
      hucMSC-Exs promoted the proliferative, migratory, and tube-forming ability of 
      HUVECs. Furthermore, overexpression of Ang-2 in hucMSC-Exs further enhanced HUVEC 
      migration and tube formation and exerted therapeutic and proangiogenic effects in 
      cutaneous wounds in rats, whereas knockdown of Ang-2 in hucMSC-Exs abrogated 
      these therapeutic and proangiogenic effects. Taken together, our results 
      indicated that hucMSC-Ex-derived Ang-2 plays a significant role in tube formation 
      of HUVECs and promotion of angiogenesis, and further suggested that 
      hucMSC-Ex-based therapy may serve as a promising therapeutic approach for 
      promoting cutaneous wound healing.
FAU - Liu, Jinwen
AU  - Liu J
AD  - Zhenjiang Key Laboratory of High Technology Research on Exosomes Foundation and 
      Transformation Application, School of Medicine, Jiangsu University, 301 Xuefu 
      Road, 212013, Zhenjiang, Jiangsu, People's Republic of China.
FAU - Yan, Zhixin
AU  - Yan Z
AD  - Affiliated Hospital of Jiangsu University, Jiangsu University, Zhenjiang, 
      Jiangsu, 212000, People's Republic of China.
FAU - Yang, Fuji
AU  - Yang F
AD  - Zhenjiang Key Laboratory of High Technology Research on Exosomes Foundation and 
      Transformation Application, School of Medicine, Jiangsu University, 301 Xuefu 
      Road, 212013, Zhenjiang, Jiangsu, People's Republic of China.
FAU - Huang, Yan
AU  - Huang Y
AD  - Zhenjiang Key Laboratory of High Technology Research on Exosomes Foundation and 
      Transformation Application, School of Medicine, Jiangsu University, 301 Xuefu 
      Road, 212013, Zhenjiang, Jiangsu, People's Republic of China.
FAU - Yu, Yao
AU  - Yu Y
AD  - Zhenjiang Key Laboratory of High Technology Research on Exosomes Foundation and 
      Transformation Application, School of Medicine, Jiangsu University, 301 Xuefu 
      Road, 212013, Zhenjiang, Jiangsu, People's Republic of China.
FAU - Zhou, Liping
AU  - Zhou L
AD  - Zhenjiang Key Laboratory of High Technology Research on Exosomes Foundation and 
      Transformation Application, School of Medicine, Jiangsu University, 301 Xuefu 
      Road, 212013, Zhenjiang, Jiangsu, People's Republic of China.
FAU - Sun, Zixuan
AU  - Sun Z
AD  - Zhenjiang Key Laboratory of High Technology Research on Exosomes Foundation and 
      Transformation Application, School of Medicine, Jiangsu University, 301 Xuefu 
      Road, 212013, Zhenjiang, Jiangsu, People's Republic of China.
FAU - Cui, Dawei
AU  - Cui D
AD  - Department of Blood Transfusion, The First Affiliated Hospital, Zhejiang 
      University School of Medicine, Zhejiang, China.
FAU - Yan, Yongmin
AU  - Yan Y
AD  - Zhenjiang Key Laboratory of High Technology Research on Exosomes Foundation and 
      Transformation Application, School of Medicine, Jiangsu University, 301 Xuefu 
      Road, 212013, Zhenjiang, Jiangsu, People's Republic of China. yym@ujs.edu.cn.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Stem Cell Rev Rep
JT  - Stem cell reviews and reports
JID - 101752767
RN  - 0 (Angiogenesis Inducing Agents)
RN  - 0 (Angiopoietin-2)
SB  - IM
MH  - *Angiogenesis Inducing Agents
MH  - Angiopoietin-2/*administration & dosage/genetics
MH  - Animals
MH  - Cell Movement
MH  - *Exosomes
MH  - Human Umbilical Vein Endothelial Cells
MH  - Humans
MH  - *Mesenchymal Stem Cells
MH  - Rats
MH  - Umbilical Cord/cytology
MH  - *Wound Healing
OTO - NOTNLM
OT  - Ang-2
OT  - Angiogenesis
OT  - Cutaneous wound healing
OT  - Exosomes
OT  - hucMSCs
EDAT- 2020/07/03 06:00
MHDA- 2022/03/04 06:00
CRDT- 2020/07/03 06:00
PHST- 2020/07/03 06:00 [pubmed]
PHST- 2022/03/04 06:00 [medline]
PHST- 2020/07/03 06:00 [entrez]
AID - 10.1007/s12015-020-09992-7 [pii]
AID - 10.1007/s12015-020-09992-7 [doi]
PST - ppublish
SO  - Stem Cell Rev Rep. 2021 Apr;17(2):305-317. doi: 10.1007/s12015-020-09992-7.