PMID- 3261736
OWN - NLM
STAT- MEDLINE
DCOM- 19880926
LR  - 20190919
IS  - 0271-9142 (Print)
IS  - 0271-9142 (Linking)
VI  - 8
IP  - 4
DP  - 1988 Jul
TI  - Anti-Klebsiella K30 phospholipid antibodies in systemic lupus erythematosus: 
      antigen cross-reactions and idiotypic sharing with antibodies to DNA and 
      Klebsiella K30 polysaccharide.
PG  - 319-31
AB  - Patients with systemic lupus erythematosus were examined for the presence of 
      serum antibodies reactive with phospholipids (PL) extracted from the membranes of 
      Klebsiella K30 (K30PL). Affinity-purified anti-K30PL antibodies were tested for 
      their ability to cross-react with other PL antigens or DNA and for the presence 
      of idiotypic markers known to be associated with anti-DNA antibodies or 
      antibodies to Klebsiella K30 polysaccharide (K30p). Affinity-purified antibodies 
      to K30PL, phosphatidylserine (PS), or phosphatidylinositol (PL) uniformly 
      cross-reacted with each other. Analysis of the PL preparations by thin-layer 
      chromatography and reversed-phase high-performance liquid chromatography (HPLC) 
      showed the presence of several components. Lupus sera reacted with one component 
      mainly in the HPLC-fractionated K30PL preparation, although this component 
      appeared to be present in the other PL preparations. Direct-binding and 
      inhibition studies showed that affinity-purified antibodies to the K30PL extract, 
      PS, or PI reacted poorly with DNA. However, the anti-K30PL antibodies possessed a 
      prominent anti-DNA idiotypic marker (AM Id) in 61% of the patients and an 
      anti-K30p idiotypic marker (SP Id) in 94% of the patients. The results thus show 
      that anti-K30PL antibodies are idiotypically related to anti-DNA and anti-K30p 
      antibody subpopulations, although they do not share the same antigen-binding 
      characteristics.
FAU - Harkiss, G D
AU  - Harkiss GD
AD  - University of Edinburgh, Department of Veterinary Pathology, UK.
FAU - Hendrie, F M
AU  - Hendrie FM
FAU - Thompson, D
AU  - Thompson D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Clin Immunol
JT  - Journal of clinical immunology
JID - 8102137
RN  - 0 (Antibodies, Antinuclear)
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Immunoglobulin Idiotypes)
RN  - 0 (Phospholipids)
RN  - 0 (Polysaccharides, Bacterial)
SB  - IM
MH  - Antibodies, Antinuclear/*analysis
MH  - Antibodies, Bacterial/*analysis
MH  - Antibody Specificity
MH  - Chromatography, High Pressure Liquid
MH  - Chromatography, Thin Layer
MH  - Cross Reactions
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Immunoglobulin Idiotypes/*analysis
MH  - Klebsiella/analysis/*immunology
MH  - Lupus Erythematosus, Systemic/*immunology/microbiology
MH  - Phospholipids/*immunology/isolation & purification
MH  - Polysaccharides, Bacterial/*immunology
MH  - Spectrum Analysis
EDAT- 1988/07/01 00:00
MHDA- 1988/07/01 00:01
CRDT- 1988/07/01 00:00
PHST- 1988/07/01 00:00 [pubmed]
PHST- 1988/07/01 00:01 [medline]
PHST- 1988/07/01 00:00 [entrez]
AID - 10.1007/BF00916560 [doi]
PST - ppublish
SO  - J Clin Immunol. 1988 Jul;8(4):319-31. doi: 10.1007/BF00916560.