PMID- 32619428
OWN - NLM
STAT- MEDLINE
DCOM- 20201026
LR  - 20201026
IS  - 1879-2650 (Electronic)
IS  - 0005-2728 (Linking)
VI  - 1861
IP  - 10
DP  - 2020 Oct 1
TI  - Regulation of light-induced H(+) extrusion and uptake by cyanobacterial homologs 
      of the plastidial FLAP1, DLDG1, and Ycf10 in Synechocystis sp. PCC6803.
PG  - 148258
LID - S0005-2728(20)30108-0 [pii]
LID - 10.1016/j.bbabio.2020.148258 [doi]
AB  - Upon a dark-to-light transition, multiple species of cyanobacteria release a 
      certain amount of H(+) from the inside to the outside of their cells. Previous 
      studies revealed the plasma membrane-localizing Proton exchange A (PxcA) is 
      involved in the light-induced H(+) extrusion in the cyanobacterium Synechocystis 
      sp. PCC6803. Among oxygenic phototrophs, two PxcA homologs are conserved; they 
      are the nuclear-encoded Day-length-dependent delayed-greening1 (DLDG1) and the 
      plastid-encoded Ycf10 in Arabidopsis thaliana. We previously identified the 
      putative DLDG1/Ycf10-interacting protein, Fluctuating-light acclimation protein1 
      (FLAP1), required for pH regulation in Arabidopsis chloroplasts. Synechocystis 
      has PxcA and FLAP1 homologs designated here as PxcA like (PxcL) and FLAP1 homolog 
      A (FlpA). Synechocystis mutants lacking pxcA, pxcL, and flpA were constructed and 
      characterized to gain more insight into regulatory mechanisms of light-induced 
      H(+) extrusion in cyanobacteria. pH change kinetics of the extracellular solvent 
      after shifting Synechocystis cells from dark to light indicated that PxcA is 
      essential for the light-induced H(+) extrusion, and both PxcA and PxcL were 
      involved in H(+) uptake. Mutational loss of flpA resulted in altered PxcA- and 
      PxcL-dependent H(+) efflux/influx activities, and the flpA-null mutant showed 
      inhibited growth under dark-light cycles, indicating the importance of FlpA 
      function for photosynthetic growth under fluctuating light. Collectively, these 
      data suggest that PxcA is involved in H(+) efflux immediately after light 
      irradiation for the rapid formation of the H(+) concentration gradient across the 
      thylakoid membranes, PxcL is involved in H(+) influx for activation of the 
      Calvin-Benson-Bassham cycle, and FlpA controls the H(+) transport under 
      fluctuating light.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Inago, Haruya
AU  - Inago H
AD  - Department of Life Science and Technology, Tokyo Institute of Technology, 
      Yokohama 226-8501, Japan.
FAU - Sato, Ryoichi
AU  - Sato R
AD  - Department of Life Science and Technology, Tokyo Institute of Technology, 
      Yokohama 226-8501, Japan.
FAU - Masuda, Shinji
AU  - Masuda S
AD  - Department of Life Science and Technology, Tokyo Institute of Technology, 
      Yokohama 226-8501, Japan. Electronic address: shmasuda@bio.titech.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200630
PL  - Netherlands
TA  - Biochim Biophys Acta Bioenerg
JT  - Biochimica et biophysica acta. Bioenergetics
JID - 101731706
RN  - 0 (Bacterial Proteins)
RN  - 0 (Protons)
SB  - IM
MH  - Bacterial Proteins/chemistry/genetics/*metabolism
MH  - Biological Transport/radiation effects
MH  - Hydrogen-Ion Concentration
MH  - *Light
MH  - Mutation
MH  - Plastids/*metabolism
MH  - *Protons
MH  - *Sequence Homology, Amino Acid
MH  - Synechocystis/genetics/*metabolism/*radiation effects
OTO - NOTNLM
OT  - Cyanobacteria
OT  - H(+) extrusion
OT  - NPQ
OT  - Photosynthesis
OT  - Synechocystis
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2020/07/04 06:00
MHDA- 2020/10/27 06:00
CRDT- 2020/07/04 06:00
PHST- 2020/04/22 00:00 [received]
PHST- 2020/06/17 00:00 [revised]
PHST- 2020/06/18 00:00 [accepted]
PHST- 2020/07/04 06:00 [pubmed]
PHST- 2020/10/27 06:00 [medline]
PHST- 2020/07/04 06:00 [entrez]
AID - S0005-2728(20)30108-0 [pii]
AID - 10.1016/j.bbabio.2020.148258 [doi]
PST - ppublish
SO  - Biochim Biophys Acta Bioenerg. 2020 Oct 1;1861(10):148258. doi: 
      10.1016/j.bbabio.2020.148258. Epub 2020 Jun 30.