PMID- 3261968
OWN - NLM
STAT- MEDLINE
DCOM- 19881011
LR  - 20190503
IS  - 0003-4967 (Print)
IS  - 1468-2060 (Electronic)
IS  - 0003-4967 (Linking)
VI  - 47
IP  - 8
DP  - 1988 Aug
TI  - Complement mediated inhibition of immune precipitation in rheumatoid arthritis: 
      studies on interaction of heat aggregated IgG with IgM rheumatoid factor.
PG  - 675-80
AB  - Serum samples from patients with seropositive rheumatoid arthritis contain an 
      inhibitor of complement mediated inhibition of immune precipitation (CMIP). This 
      inhibitory effect can be produced by the addition of either purified monoclonal 
      or polyclonal IgM rheumatoid factor (RF) to human serum. The specificity of the 
      rheumatoid factor influences the degree of inhibition, and when precipitation 
      occurs the rheumatoid factor coprecipitates with the antigen-antibody complex. In 
      rheumatoid sera there was a significant positive correlation between IgM RF 
      concentration and inhibitory activity, though the range of inhibitory activity 
      seen for the same concentration of rheumatoid factor was considerable. Small 
      quantities of heat aggregated IgG (HAGG) had a much greater effect on the 
      measurement in an enzyme linked immunosorbent assay (ELISA) of IgM RF than they 
      did on the inhibitory activity of IgM RF in the CMIP assay. Larger quantities of 
      HAGG initiated complement activation and increased the precipitation of immune 
      complexes. IgM RF reduced the complement activating properties of HAGG by 
      reducing the amount of Clq which bound to the aggregate. The mechanisms by which 
      IgM RF overcomes CMIP in rheumatoid sera may involve its inhibitory effects on 
      the binding of Cl to the antigen-antibody complex.
FAU - O'Sullivan, M M
AU  - O'Sullivan MM
AD  - Department of Rheumatology, University Hospital of Wales, Cardiff.
FAU - Amos, N
AU  - Amos N
FAU - Williams, B D
AU  - Williams BD
LA  - eng
PT  - Journal Article
PL  - England
TA  - Ann Rheum Dis
JT  - Annals of the rheumatic diseases
JID - 0372355
RN  - 0 (Antigen-Antibody Complex)
RN  - 0 (Complement C1)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Immunoglobulin M)
RN  - 80295-33-6 (Complement C1q)
RN  - 9009-79-4 (Rheumatoid Factor)
RN  - EC 3.- (Complement Activating Enzymes)
SB  - IM
MH  - Antigen-Antibody Complex/immunology
MH  - Arthritis, Rheumatoid/*immunology
MH  - Chemical Precipitation
MH  - Complement Activating Enzymes/immunology
MH  - *Complement Activation
MH  - Complement C1/immunology
MH  - Complement C1q
MH  - Humans
MH  - Immunoglobulin G/*immunology
MH  - Immunoglobulin M/*immunology
MH  - Rheumatoid Factor/*immunology
PMC - PMC1006721
EDAT- 1988/08/01 00:00
MHDA- 1988/08/01 00:01
PMCR- 1991/08/01
CRDT- 1988/08/01 00:00
PHST- 1988/08/01 00:00 [pubmed]
PHST- 1988/08/01 00:01 [medline]
PHST- 1988/08/01 00:00 [entrez]
PHST- 1991/08/01 00:00 [pmc-release]
AID - 10.1136/ard.47.8.675 [doi]
PST - ppublish
SO  - Ann Rheum Dis. 1988 Aug;47(8):675-80. doi: 10.1136/ard.47.8.675.