PMID- 32619814
OWN - NLM
STAT- MEDLINE
DCOM- 20210621
LR  - 20210621
IS  - 1879-3231 (Electronic)
IS  - 0093-691X (Linking)
VI  - 154
DP  - 2020 Sep 15
TI  - The advantages of using cytospins of uterine lavage fluid for the diagnosis of 
      equine endometritis.
PG  - 143-151
LID - S0093-691X(20)30286-7 [pii]
LID - 10.1016/j.theriogenology.2020.05.008 [doi]
AB  - Uterine lavage (UL) is a routine diagnostic procedure for endometritis. In UL the 
      fluid is centrifuged and the sediment smeared. Samples prepared in 
      cytocentrifuges, the so-called "cytospins", are useful for evaluating cells in 
      fluids, but never been used in UL. The aim of this study was to assess the 
      usefulness of cytospins after UL, comparing automatic versus manual 
      cytocentrifuges, and to determine their value for the diagnosis of endometritis. 
      The study was divided in two parts. Firstly, UL was performed in 16 mares and a 
      small part of the retrieved fluid was cytocentrifuged in an automatic (PreCyto) 
      and manual (PreMan) cytocentrifuge, whereas the remaining fluid was centrifuged. 
      After that, the sediment was divided into three quotas. One quota was smeared, 
      one was processed in an automatic cytocentrifuge (PostCyto) and the last quota 
      was cytospinned in a manual apparatus (PostMan). Cytospins obtained were scored 
      for cellularity, cell preservation, presence of inflammatory cells, bacteria and 
      contaminants; results were compared with sediment smears. Secondly in this study, 
      the best cytospin method was compared with sediment smears in another group of 13 
      mares, which had endometrial biopsy after UL. Agreement between sediment smears 
      and cytospins was poor to moderate. Compared to sediment smears, cytospins were 
      more cellular, with better morphological details. Urine crystals and fecal 
      contamination were detected more often in cytospins (especially PostCyto and 
      PostMan). No differences in the percentage of inflammatory or epithelial cells 
      existed. PostMan was considered the best method to evaluate UL fluid and it had 
      higher sensitivity (80%), compared with sediment smears (60%), for diagnosing 
      endometritis. Cytocentrifugation offers significant advantages over sediment 
      smears and the manual cytocentrifuge is well suited for horse stable conditions.
CI  - Copyright (c) 2020 Elsevier Inc. All rights reserved.
FAU - Marcos, Ricardo
AU  - Marcos R
AD  - Cytology Diagnostic Services, Laboratory of Histology and Embryology, Institute 
      of Biomedical Sciences Abel Salazar, University of Porto (ICBAS-UP), Portugal. 
      Electronic address: rmarcos@icbas.up.pt.
FAU - Ribeiro, Tiago
AU  - Ribeiro T
AD  - Animal Reproduction Center of Vairao, ICBAS-UP, Portugal.
FAU - Santos, Marta
AU  - Santos M
AD  - Cytology Diagnostic Services, Laboratory of Histology and Embryology, Institute 
      of Biomedical Sciences Abel Salazar, University of Porto (ICBAS-UP), Portugal.
FAU - Correia-Gomes, Carla
AU  - Correia-Gomes C
AD  - Epidemiology Research Unit, Department of Veterinary and Animal Science, Northern 
      Faculty, Scotland's Rural College (SRUC), United Kingdom.
FAU - Macedo, Sonia
AU  - Macedo S
AD  - Animal Reproduction Center of Vairao, ICBAS-UP, Portugal.
FAU - Guimaraes, Tiago
AU  - Guimaraes T
AD  - Animal Reproduction Center of Vairao, ICBAS-UP, Portugal.
FAU - Blielbernicht, Miguel
AU  - Blielbernicht M
AD  - EmbrioVet, Salvaterra de Magos, Portugal.
FAU - Caniatti, Mario
AU  - Caniatti M
AD  - DIMEVET Dipartimento di Medicina Veterinaria, Universita degli Studi di Milano, 
      Italy.
FAU - Rocha, Antonio
AU  - Rocha A
AD  - Animal Reproduction Center of Vairao, ICBAS-UP, Portugal.
LA  - eng
PT  - Journal Article
DEP - 20200521
PL  - United States
TA  - Theriogenology
JT  - Theriogenology
JID - 0421510
SB  - IM
MH  - Animals
MH  - Centrifugation/veterinary
MH  - *Endometritis/diagnosis/veterinary
MH  - Endometrium
MH  - Female
MH  - *Horse Diseases/diagnosis
MH  - Horses
MH  - Therapeutic Irrigation/veterinary
OTO - NOTNLM
OT  - Cytology
OT  - Endometritis
OT  - Horse
OT  - Uterine lavage
COIS- Declaration of competing interest The authors declare that they have no conflict 
      of interest.
EDAT- 2020/07/04 06:00
MHDA- 2021/06/22 06:00
CRDT- 2020/07/04 06:00
PHST- 2019/12/11 00:00 [received]
PHST- 2020/05/05 00:00 [revised]
PHST- 2020/05/05 00:00 [accepted]
PHST- 2020/07/04 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2020/07/04 06:00 [entrez]
AID - S0093-691X(20)30286-7 [pii]
AID - 10.1016/j.theriogenology.2020.05.008 [doi]
PST - ppublish
SO  - Theriogenology. 2020 Sep 15;154:143-151. doi: 
      10.1016/j.theriogenology.2020.05.008. Epub 2020 May 21.