PMID- 32621335
OWN - NLM
STAT- MEDLINE
DCOM- 20210803
LR  - 20210803
IS  - 1098-2825 (Electronic)
IS  - 0887-8013 (Print)
IS  - 0887-8013 (Linking)
VI  - 34
IP  - 10
DP  - 2020 Oct
TI  - CTLA-4 and HLA-DQ are key molecules in the regulation of mDC-mediated cellular 
      immunity by Tregs in severe aplastic anemia.
PG  - e23443
LID - 10.1002/jcla.23443 [doi]
LID - e23443
AB  - BACKGROUND: Regulatory T cells (Tregs) inhibit the activation of cluster of 
      differentiation (CD) 4(+) , CD8(+) T cells and the antigen-presenting process of 
      antigen-presenting cells, and may play an important role in acquired severe 
      aplastic anemia (SAA). METHODS: Flow cytometry was used to measure CD4(+) CD25(+) 
      CD127(dim) Tregs, cytotoxic T lymphocyte antigen 4 (CTLA-4) expression on Tregs, 
      and human leukocyte antigen (HLA)-DQ expression on myeloid dendritic cells 
      (mDCs). The correlations of CTLA-4 and HLA-DQ with immune status and clinical 
      indicators and the changes in these indicators after immunosuppressive therapy 
      (IST) were analyzed. RESULTS: In SAA patients, the number of Tregs and their 
      CTLA-4 expression were low but recovered after IST; the HLA-DQ expression on mDCs 
      was high but decreased after IST. The CTLA-4 expression on Tregs and the HLA-DQ 
      expression on mDCs showed a negative correlation. The CTLA-4 on Tregs was 
      positively but HLA-DQ on mDCs negatively correlated with the number of Tregs, 
      natural killer (NK) cell number, and CD4(+) T/CD8(+) T ratio. CTLA-4 was 
      positively but HLA-DQ negatively correlated with the percentage of granulocytoid 
      and erythroid cells in bone marrow, white blood cell count in PB, absolute 
      neutrophil count in PB, and the percentage of reticulocytes in PB. CONCLUSIONS: 
      CTLA-4/HLA-DQ may be key in the regulation of Tregs on mDCs in SAA patients. Our 
      findings should be helpful for further investigation of the mechanism of immune 
      pathogenesis in SAA patients. Studies on the regulators of Treg and CTLA-4 
      activity will be valuable for SAA therapeutic target research and disease 
      monitoring.
CI  - (c) 2020 The Authors. Journal of Clinical Laboratory Analysis published by Wiley 
      Periodicals LLC.
FAU - Liu, Bingnan
AU  - Liu B
AUID- ORCID: 0000-0002-1938-4679
AD  - Department of Hematology, Tianjin Medical University General Hospital, Tianjin, 
      PR China.
FAU - Shao, Yuanyuan
AU  - Shao Y
AD  - Department of Hematology, Tianjin Medical University General Hospital, Tianjin, 
      PR China.
FAU - Liang, Xiaowei
AU  - Liang X
AD  - Department of Hematology, Tianjin Medical University General Hospital, Tianjin, 
      PR China.
FAU - Lu, Dan
AU  - Lu D
AD  - Department of Hematology, Tianjin Medical University General Hospital, Tianjin, 
      PR China.
FAU - Yan, Li
AU  - Yan L
AD  - Department of Hematology, Tianjin Medical University General Hospital, Tianjin, 
      PR China.
FAU - Churov, Alexey
AU  - Churov A
AUID- ORCID: 0000-0002-3920-7581
AD  - Department of Hematology, Tianjin Medical University General Hospital, Tianjin, 
      PR China.
FAU - Fu, Rong
AU  - Fu R
AD  - Department of Hematology, Tianjin Medical University General Hospital, Tianjin, 
      PR China.
LA  - eng
GR  - 16JCZDJC35300/Natural Science Foundation of Tianjin City/
GR  - 17JCQNJC11500/Natural Science Foundation of Tianjin City/
GR  - 18JCYBJC91700/Natural Science Foundation of Tianjin City/
GR  - 18ZXDBSY00140/Natural Science Foundation of Tianjin City/
GR  - 81700117/National Natural Science Foundation of China/
GR  - 81700118/National Natural Science Foundation of China/
GR  - 81770110/National Natural Science Foundation of China/
GR  - 81770118/National Natural Science Foundation of China/
GR  - 81800119/National Natural Science Foundation of China/
GR  - 81800120/National Natural Science Foundation of China/
GR  - 81870101/National Natural Science Foundation of China/
GR  - 81900125/National Natural Science Foundation of China/
GR  - 81970115/National Natural Science Foundation of China/
GR  - 81970116/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20200703
PL  - United States
TA  - J Clin Lab Anal
JT  - Journal of clinical laboratory analysis
JID - 8801384
RN  - 0 (CTLA-4 Antigen)
RN  - 0 (HLA-DQ Antigens)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Anemia, Aplastic/*immunology
MH  - CTLA-4 Antigen/*metabolism
MH  - Child
MH  - Dendritic Cells/*immunology
MH  - Female
MH  - HLA-DQ Antigens/*metabolism
MH  - Humans
MH  - *Immunity, Cellular
MH  - Killer Cells, Natural/immunology
MH  - Lymphocyte Count
MH  - Male
MH  - Middle Aged
MH  - T-Lymphocytes, Regulatory/*immunology
MH  - Young Adult
PMC - PMC7595925
OTO - NOTNLM
OT  - cytotoxic T lymphocyte antigen 4
OT  - human leukocyte antigen
OT  - regulatory T cells
OT  - severe aplastic anemia
COIS- The authors declare no conflicts of interest.
EDAT- 2020/07/06 06:00
MHDA- 2021/08/04 06:00
PMCR- 2020/07/03
CRDT- 2020/07/05 06:00
PHST- 2020/05/06 00:00 [received]
PHST- 2020/06/01 00:00 [revised]
PHST- 2020/06/03 00:00 [accepted]
PHST- 2020/07/06 06:00 [pubmed]
PHST- 2021/08/04 06:00 [medline]
PHST- 2020/07/05 06:00 [entrez]
PHST- 2020/07/03 00:00 [pmc-release]
AID - JCLA23443 [pii]
AID - 10.1002/jcla.23443 [doi]
PST - ppublish
SO  - J Clin Lab Anal. 2020 Oct;34(10):e23443. doi: 10.1002/jcla.23443. Epub 2020 Jul 
      3.