PMID- 32622342
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240227
IS  - 1876-7753 (Electronic)
IS  - 1873-5061 (Linking)
VI  - 47
DP  - 2020 Jun 26
TI  - Tissues derived from reprogrammed Wharton's jelly stem cells of the umbilical 
      cord as a platform to study gestational diabetes mellitus.
PG  - 101880
LID - S1873-5061(20)30181-1 [pii]
LID - 10.1016/j.scr.2020.101880 [doi]
AB  - Gestational diabetes mellitus (GDM) has been strongly associated with an 
      increased risk of type 2 diabetes mellitus (T2DM) in later child and adulthood. 
      The human umbilical cord and its contents are of fetal origin and represent the 
      fetus genetically and physiologically. Since it is not possible to obtain tissues 
      from the fetus and newborn to investigate the association between GDM and later 
      T2DM, we reprogrammed the stem cells from the Wharton's jelly of umbilical cords 
      (hWJSCs) of GDM and non-GDM mothers into induced pluripotent stem cells (iPSCs) 
      and then differentiated the iPSCs into insulin-producing cells (IPCs) to provide 
      pancreatic tissues that represent the fetus of GDM and normal mothers. These 
      tissues are an attractive model to study the effects of glucose on the fetus. 
      Interestingly, GDM-iPSCs had a decreased potential towards differentiation into 
      IPCs. IPCs differentiated from GDM-iPSCs also had lower total insulin content and 
      a lower capacity for insulin secretion to glucose stimulation compared to their 
      normal-iPSC counterparts. This abnormal pathogenesis in GDM-iPSCs pancreatic 
      differentiation recapitulates the pathology that may be observed in the infants 
      of the diabetic mother (IDM) and while indicating adaptive mechanisms for fetal 
      survival, may lead to the development of T2DM later in life. (199 words).
CI  - Copyright (c) 2020 The Author(s). Published by Elsevier B.V. All rights reserved.
FAU - Kong, Chiou Mee
AU  - Kong CM
AD  - Department of Obstetrics & Gynaecology, Yong Loo Lin School of Medicine, National 
      University Health System (NUHS), National University of Singapore, 1E Kent Ridge 
      Rd, Singapore 119228, Singapore.
FAU - Arjunan, Subramanian
AU  - Arjunan S
AD  - Department of Obstetrics & Gynaecology, Yong Loo Lin School of Medicine, National 
      University Health System (NUHS), National University of Singapore, 1E Kent Ridge 
      Rd, Singapore 119228, Singapore.
FAU - Gan, Shu Uin
AU  - Gan SU
AD  - Department of Surgery, Yong Loo Lin School of Medicine, National University 
      Health System (NUHS), National University of Singapore, 1E Kent Ridge Rd, 
      Singapore 119228, Singapore.
FAU - Biswas, Arijit
AU  - Biswas A
AD  - Department of Obstetrics & Gynaecology, Yong Loo Lin School of Medicine, National 
      University Health System (NUHS), National University of Singapore, 1E Kent Ridge 
      Rd, Singapore 119228, Singapore.
FAU - Bongso, Ariff
AU  - Bongso A
AD  - Department of Obstetrics & Gynaecology, Yong Loo Lin School of Medicine, National 
      University Health System (NUHS), National University of Singapore, 1E Kent Ridge 
      Rd, Singapore 119228, Singapore.
FAU - Fong, Chui-Yee
AU  - Fong CY
AD  - Department of Obstetrics & Gynaecology, Yong Loo Lin School of Medicine, National 
      University Health System (NUHS), National University of Singapore, 1E Kent Ridge 
      Rd, Singapore 119228, Singapore.
LA  - eng
PT  - Journal Article
DEP - 20200626
PL  - England
TA  - Stem Cell Res
JT  - Stem cell research
JID - 101316957
SB  - IM
OTO - NOTNLM
OT  - Gestational diabetes mellitus
OT  - Induced pluripotent stem cells
OT  - Pancreatic cells
OT  - Reprogramming
OT  - Wharton's jelly stem cells
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2020/07/06 06:00
MHDA- 2020/07/06 06:01
CRDT- 2020/07/05 06:00
PHST- 2019/12/23 00:00 [received]
PHST- 2020/05/04 00:00 [revised]
PHST- 2020/06/14 00:00 [accepted]
PHST- 2020/07/06 06:01 [medline]
PHST- 2020/07/06 06:00 [pubmed]
PHST- 2020/07/05 06:00 [entrez]
AID - S1873-5061(20)30181-1 [pii]
AID - 10.1016/j.scr.2020.101880 [doi]
PST - aheadofprint
SO  - Stem Cell Res. 2020 Jun 26;47:101880. doi: 10.1016/j.scr.2020.101880.