PMID- 32623908
OWN - NLM
STAT- MEDLINE
DCOM- 20210625
LR  - 20210625
IS  - 1555-3892 (Electronic)
IS  - 0963-6897 (Print)
IS  - 0963-6897 (Linking)
VI  - 29
DP  - 2020 Jan-Dec
TI  - Umbilical Cord Blood Units Cryopreserved in the Public Cord Blood Bank: A 
      Breakthrough in iPSC Haplobanking?
PG  - 963689720926151
LID - 10.1177/0963689720926151 [doi]
LID - 0963689720926151
AB  - The use of induced pluripotent stem cells (iPSCs) is an emerging therapeutic 
      option for precision medicine. Cord blood (CB) cells with lower immunogenicity, 
      fewer genomic changes, and persistent epigenetic memory might be ideal candidates 
      for iPSC production. Based on the human leukocyte antigen (HLA) distribution of 
      cord blood units (CBUs) in the public CB bank, we estimated the coverage of the 
      Korean population with HLA-homozygous iPSCs to repurpose cryopreserved CBUs. We 
      analyzed a total of 27,904 Korean CBUs donated to the public CB bank. 
      Low-to-intermediate resolution typing was performed for HLA-A, -B, and -DRB1 
      alleles, and individuals possessing homozygous HLA haplotypes were identified by 
      direct counting. Moreover, the matching probabilities for zero-mismatch 
      transplantation were calculated for 27,904 CBUs and 50,000,000 potential Korean 
      patients. Among the preserved CBUs, 15 HLA-A, 40 HLA-B, and 13 HLA-DRB1 alleles 
      as well as 48 homozygous HLA-A-B-DRB1 haplotypes were identified at serological 
      equivalents (2 digits). The 48 identified homozygous haplotypes cumulatively 
      matched 78.18% of the 27,904 Korean CB donors as zero HLA-mismatch iPSC sources. 
      Among the combinations of 1,699 haplotypes with frequencies greater than 0.001%, 
      assuming a population of 50 million, those 48 haplotypes can provide a match for 
      78.37% of potential Korean recipients. A practicable number of HLA-A, -B, and 
      -DRB1 homozygous iPSC lines derived from CBUs may be an efficient option in 
      allogeneic iPSC therapy because this type of haplobanking may provide cell lines 
      with optimal HLA matching for up to three-quarters of the Korean population.
FAU - Roh, Eun Youn
AU  - Roh EY
AUID- ORCID: 0000-0002-1787-7509
AD  - Department of Laboratory Medicine, Seoul National University Boramae Medical 
      Center, Republic of Korea.
AD  - Seoul Metropolitan Public Cord Blood Bank-ALLCORD, Republic of Korea.
AD  - Department of Laboratory Medicine, Seoul National University College of Medicine, 
      Republic of Korea.
AD  - Both the authors contributed equally to this study as co-first authors.
FAU - Oh, Sohee
AU  - Oh S
AD  - Department of Biostatistics, Seoul National University Boramae Medical Center, 
      Republic of Korea.
AD  - Both the authors contributed equally to this study as co-first authors.
FAU - Yoon, Jong Hyun
AU  - Yoon JH
AD  - Department of Laboratory Medicine, Seoul National University Boramae Medical 
      Center, Republic of Korea.
AD  - Seoul Metropolitan Public Cord Blood Bank-ALLCORD, Republic of Korea.
AD  - Department of Laboratory Medicine, Seoul National University College of Medicine, 
      Republic of Korea.
FAU - Kim, Byoung Jae
AU  - Kim BJ
AD  - Seoul Metropolitan Public Cord Blood Bank-ALLCORD, Republic of Korea.
AD  - Department of Obstetrics and Gynecology, Seoul National University Boramae 
      Medical Center, Republic of Korea.
FAU - Song, Eun Young
AU  - Song EY
AD  - Department of Laboratory Medicine, Seoul National University College of Medicine, 
      Republic of Korea.
FAU - Shin, Sue
AU  - Shin S
AD  - Department of Laboratory Medicine, Seoul National University Boramae Medical 
      Center, Republic of Korea.
AD  - Seoul Metropolitan Public Cord Blood Bank-ALLCORD, Republic of Korea.
AD  - Department of Laboratory Medicine, Seoul National University College of Medicine, 
      Republic of Korea.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cell Transplant
JT  - Cell transplantation
JID - 9208854
SB  - IM
MH  - Blood Banks/*standards
MH  - Cryopreservation/*methods
MH  - Fetal Blood/*chemistry
MH  - Humans
MH  - Induced Pluripotent Stem Cells/*metabolism
PMC - PMC7563803
OTO - NOTNLM
OT  - Korean
OT  - cord blood
OT  - haplobanking
OT  - homozygous
OT  - human leukocyte antigen (HLA)
OT  - iPSC (induced pluripotent stem cell)
COIS- Declaration of Conflicting Interests: The author(s) declared no potential 
      conflicts of interest with respect to the research, authorship, and/or 
      publication of this article.
EDAT- 2020/07/07 06:00
MHDA- 2021/06/29 06:00
PMCR- 2020/07/06
CRDT- 2020/07/07 06:00
PHST- 2020/07/07 06:00 [entrez]
PHST- 2020/07/07 06:00 [pubmed]
PHST- 2021/06/29 06:00 [medline]
PHST- 2020/07/06 00:00 [pmc-release]
AID - 10.1177_0963689720926151 [pii]
AID - 10.1177/0963689720926151 [doi]
PST - ppublish
SO  - Cell Transplant. 2020 Jan-Dec;29:963689720926151. doi: 10.1177/0963689720926151.