PMID- 3262473
OWN - NLM
STAT- MEDLINE
DCOM- 19881121
LR  - 20190820
IS  - 0090-1229 (Print)
IS  - 0090-1229 (Linking)
VI  - 49
IP  - 2
DP  - 1988 Nov
TI  - Effect of aggregated immunoglobulins on mononuclear cell protein production in 
      rheumatoid arthritis.
PG  - 318-25
AB  - The synthesis and secretion of proteins, especially interleukin 1 (IL-1), by 
      mononuclear cells from patients with rheumatoid arthritis (RA) in response to 
      heat-aggregated IgG (HAGG) has been investigated. Mononuclear cells were labeled 
      with 35S-methionine during 1 hr of HAGG stimulation, and the intracellular and 
      extracellular protein content was measured and compared by fluorography. HAGG 
      dramatically accelerated protein release into the medium. This was observed in 
      both normal and RA mononuclear cells but was much more marked with the latter. 
      The phenomenon was correlated with the release of a IL-1 activity measurable in 
      the thymocyte proliferation assay with a more pronounced labeling in the 
      extracellular medium of a 14-kDa band not detectable in the intracellular 
      pattern. Partially purified proteins between 14-20 kDa recovered by transblotting 
      also stimulated thymocyte proliferation. These results therefore suggest a 
      particular sensitivity of RA mononuclear cells to HAGG, resulting in a very rapid 
      de novo synthesis and an accelerated secretion rate of IL-1.
FAU - Mairesse, N
AU  - Mairesse N
AD  - Division of Rheumatology, Erasmus University Hospital, Brussels, Belgium.
FAU - Ameryckx, G
AU  - Ameryckx G
FAU - Pierart, M
AU  - Pierart M
FAU - Appelboom, T
AU  - Appelboom T
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Clin Immunol Immunopathol
JT  - Clinical immunology and immunopathology
JID - 0356637
RN  - 0 (Immunoglobulins)
RN  - 0 (Interleukin-1)
RN  - 0 (Macromolecular Substances)
SB  - IM
MH  - Adult
MH  - Arthritis, Rheumatoid/*immunology/metabolism
MH  - Female
MH  - Hot Temperature
MH  - Humans
MH  - Immunoglobulins/*physiology
MH  - Interleukin-1/biosynthesis/isolation & purification
MH  - Intracellular Fluid/metabolism
MH  - Kinetics
MH  - Leukocytes, Mononuclear/*metabolism
MH  - Macromolecular Substances
MH  - Male
MH  - Middle Aged
MH  - Molecular Weight
MH  - *Protein Biosynthesis
EDAT- 1988/11/01 00:00
MHDA- 1988/11/01 00:01
CRDT- 1988/11/01 00:00
PHST- 1988/11/01 00:00 [pubmed]
PHST- 1988/11/01 00:01 [medline]
PHST- 1988/11/01 00:00 [entrez]
AID - 10.1016/0090-1229(88)90122-5 [doi]
PST - ppublish
SO  - Clin Immunol Immunopathol. 1988 Nov;49(2):318-25. doi: 
      10.1016/0090-1229(88)90122-5.