PMID- 32626831
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 2471-254X (Electronic)
IS  - 2471-254X (Linking)
VI  - 4
IP  - 7
DP  - 2020 Jul
TI  - HBV-RNA Co-amplification May Influence HBV DNA Viral Load Determination.
PG  - 983-997
LID - 10.1002/hep4.1520 [doi]
AB  - Despite effective hepatitis B virus (HBV)-DNA suppression, HBV RNA can circulate 
      in patients receiving nucleoside/nucleotide analogues (NAs). Current assays 
      quantify HBV DNA by either real-time polymerase chain reaction (PCR), which uses 
      DNA polymerase, or transcription-mediated amplification, which uses 
      reverse-transcriptase (RT) and RNA polymerase. We assessed the effect of RT 
      capability on HBV-DNA quantification in samples from three cohorts, including 
      patients with quantified HBV RNA. We compared the HBV-DNA levels by real-time PCR 
      (cobas HBV, Roche 6800/8800; Xpert HBV, Cepheid), transcription-mediated 
      amplification (Aptima HBV, Hologic), and real-time PCR with added RT capability 
      (cobas HBV+RT). In the first cohort (n = 45) followed over 192 weeks of NA 
      therapy, on-treatment HBV-DNA levels were higher with cobas HBV+RT than cobas HBV 
      (mean difference: 0.14 log(10) IU/mL). In a second cohort (n = 50) followed over 
      96 weeks of NA therapy, HBV-DNA viral load was significantly higher with the 
      cobas HBV+RT and Aptima HBV compared with the cobas HBV test at all time points 
      after initiation of NA therapy (mean difference: 0.65-1.16 log(10) IU/mL). A 
      clinically significant difference was not detected between the assays at 
      baseline. In a third cohort (n = 53), after a median of 2.2 years of NA therapy, 
      we detected HBV RNA (median 5.6 log(10) copies/mL) in 23 patients (43.4%). Median 
      HBV-DNA levels by Aptima HBV were 2.4 versus less than 1 log(10) IU/mL in samples 
      with HBV RNA and without HBV RNA, respectively (P = 0.0006). In treated patients 
      with HBV RNA, Aptima HBV measured higher HBV-DNA levels than Xpert HBV and cobas 
      HBV. Conclusion: Tests including an RT step may overestimate HBV DNA, 
      particularly in samples with low viral loads as a result of NA therapy. This 
      overestimation is likely due to amplification of HBV RNA and may have an impact 
      on clinical decisions.
CI  - (c) 2020 The Authors. Hepatology Communications published by Wiley Periodicals, 
      Inc., on behalf of the American Association for the Study of Liver Diseases.
FAU - Maasoumy, Benjamin
AU  - Maasoumy B
AUID- ORCID: 0000-0002-3250-3177
AD  - Department of Gastroenterology, Hepatology and Endocrinology Hannover Medical 
      School Hannover Germany.
AD  - Center for Infection Research Hannover-Braunschweig site Braunschweig Germany.
FAU - Geretti, Anna Maria
AU  - Geretti AM
AD  - Institute of Infection University of Liverpool Liverpool United Kingdom.
FAU - Frontzek, Andre
AU  - Frontzek A
AD  - Labor Stein Monchengladbach Germany.
FAU - Austin, Harrison
AU  - Austin H
AD  - Institute of Infection University of Liverpool Liverpool United Kingdom.
FAU - Aretzweiler, Gudrun
AU  - Aretzweiler G
AD  - Labor Stein Monchengladbach Germany.
FAU - Garcia-Alvarez, Monica
AU  - Garcia-Alvarez M
AD  - Hospital Universitario 12 de Octubre Madrid Spain.
FAU - Leuchter, Susanne
AU  - Leuchter S
AD  - Labor Stein Monchengladbach Germany.
FAU - Simon, Christian O
AU  - Simon CO
AD  - Roche Molecular Systems Pleasanton CA.
FAU - Marins, Ed G
AU  - Marins EG
AD  - Roche Molecular Systems Pleasanton CA.
FAU - Canchola, Jesse A
AU  - Canchola JA
AD  - Roche Molecular Systems Pleasanton CA.
FAU - Cornberg, Markus
AU  - Cornberg M
AD  - Department of Gastroenterology, Hepatology and Endocrinology Hannover Medical 
      School Hannover Germany.
AD  - Center for Infection Research Hannover-Braunschweig site Braunschweig Germany.
AD  - Center for Individualized Infection Medicine Hannover Germany.
FAU - Delgado, Rafael
AU  - Delgado R
AD  - Hospital Universitario 12 de Octubre Madrid Spain.
FAU - Wedemeyer, Heiner
AU  - Wedemeyer H
AD  - Department of Gastroenterology, Hepatology and Endocrinology Hannover Medical 
      School Hannover Germany.
AD  - Essen University Hospital University of Duisburg-Essen Essen Germany.
LA  - eng
PT  - Journal Article
DEP - 20200526
PL  - United States
TA  - Hepatol Commun
JT  - Hepatology communications
JID - 101695860
PMC - PMC7327219
EDAT- 2020/07/07 06:00
MHDA- 2020/07/07 06:01
PMCR- 2020/05/26
CRDT- 2020/07/07 06:00
PHST- 2019/08/12 00:00 [received]
PHST- 2020/03/13 00:00 [revised]
PHST- 2020/03/18 00:00 [accepted]
PHST- 2020/07/07 06:00 [entrez]
PHST- 2020/07/07 06:00 [pubmed]
PHST- 2020/07/07 06:01 [medline]
PHST- 2020/05/26 00:00 [pmc-release]
AID - HEP41520 [pii]
AID - 10.1002/hep4.1520 [doi]
PST - epublish
SO  - Hepatol Commun. 2020 May 26;4(7):983-997. doi: 10.1002/hep4.1520. eCollection 
      2020 Jul.