PMID- 32626953
OWN - NLM
STAT- MEDLINE
DCOM- 20210308
LR  - 20210308
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Print)
IS  - 1107-3756 (Linking)
VI  - 46
IP  - 2
DP  - 2020 Aug
TI  - Exosomal microRNA‑146a derived from mesenchymal stem cells increases the 
      sensitivity of ovarian cancer cells to docetaxel and taxane via a LAMC2‑mediated 
      PI3K/Akt axis.
PG  - 609-620
LID - 10.3892/ijmm.2020.4634 [doi]
AB  - The carrier role of exosomes from human umbilical cord mesenchymal stem cells 
      (hUCMSCs) containing microRNAs (miRNAs) has been implicated in gene and drug 
      therapy. The aim of the present study was to investigate the role of exosomal 
      microRNA‑146a (miR‑146a) from hUCMSCs in ovarian cancer (OC). Following the 
      generation of docetaxel (DTX)‑resistant SKOV3 cells and taxane‑resistant A2780 
      cells, exosomes were isolated from hUCMSCs and added to the chemoresistant cells. 
      Microarray analysis revealed that miR‑146a expression was upregulated in 
      DTX/SKOV3 cells among 15 ectopically expressed miRNAs. Analysis using the 
      StarBase and miRSearch databases demonstrated that miR‑146a targeted laminin gamma2 
      (LAMC2), which was further verified using dual‑luciferase reporter assays. 
      Subsequently, miR‑146a inhibitor or LAMC2 overexpression vectors were transfected 
      into hUCMSCs or OC cells, respectively, and their effects on growth and 
      chemoresistance in OC cells were assessed. The hUCMSC‑derived exosomes reduced 
      cell growth and chemoresistance in OC. Furthermore, hUCMSC‑derived exosomes with 
      miR‑146a expression knocked down increased OC cell growth and chemoresistance, 
      which was mediated by the PI3K/Akt signaling pathway via LAMC2.
FAU - Qiu, Liya
AU  - Qiu L
AD  - Department of Obstetrics and Gynecology, Taizhou Women and Children's Hospital, 
      Taizhou, Zhejiang 318000, P.R. China.
FAU - Wang, Jiakun
AU  - Wang J
AD  - Department of Obstetrics and Gynecology, Taizhou Women and Children's Hospital, 
      Taizhou, Zhejiang 318000, P.R. China.
FAU - Chen, Mei
AU  - Chen M
AD  - Department of Obstetrics and Gynecology, Taizhou Women and Children's Hospital, 
      Taizhou, Zhejiang 318000, P.R. China.
FAU - Chen, Fengyun
AU  - Chen F
AD  - Department of Obstetrics and Gynecology, Taizhou Women and Children's Hospital, 
      Taizhou, Zhejiang 318000, P.R. China.
FAU - Tu, Wenluo
AU  - Tu W
AD  - Department of Obstetrics and Gynecology, Taizhou Women and Children's Hospital, 
      Taizhou, Zhejiang 318000, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20200605
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (LAMC2 protein, human)
RN  - 0 (Laminin)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Apoptosis/genetics/physiology
MH  - Blotting, Western
MH  - Computational Biology/*methods
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Exosomes/genetics/metabolism
MH  - Female
MH  - Humans
MH  - Laminin/genetics/metabolism
MH  - MicroRNAs/genetics/metabolism
MH  - Ovarian Neoplasms/genetics/*metabolism
PMC - PMC7307828
EDAT- 2020/07/07 06:00
MHDA- 2021/03/09 06:00
PMCR- 2020/06/05
CRDT- 2020/07/07 06:00
PHST- 2020/02/12 00:00 [received]
PHST- 2020/05/04 00:00 [accepted]
PHST- 2020/07/07 06:00 [entrez]
PHST- 2020/07/07 06:00 [pubmed]
PHST- 2021/03/09 06:00 [medline]
PHST- 2020/06/05 00:00 [pmc-release]
AID - ijmm-46-02-0609 [pii]
AID - 10.3892/ijmm.2020.4634 [doi]
PST - ppublish
SO  - Int J Mol Med. 2020 Aug;46(2):609-620. doi: 10.3892/ijmm.2020.4634. Epub 2020 Jun 
      5.