PMID- 32627718
OWN - NLM
STAT- MEDLINE
DCOM- 20201231
LR  - 20210706
IS  - 1943-4936 (Electronic)
IS  - 1040-6387 (Print)
IS  - 1040-6387 (Linking)
VI  - 32
IP  - 5
DP  - 2020 Sep
TI  - Validation of a commercial magnetic bead-based multiplex assay for 5 novel 
      biomarkers of acute kidney injury in canine serum.
PG  - 656-663
LID - 10.1177/1040638720939520 [doi]
AB  - Interest is growing in measurement of novel biomarkers for the diagnosis of acute 
      kidney injury. Multiplex assays may provide a rapid and cost-effective way of 
      measurement; however, sparse information is published regarding their use in 
      dogs. We aimed to validate a commercial magnetic bead-based assay for 5 
      biomarkers: clusterin (Clus), cystatin C (CysC), kidney injury molecule 1 
      (KIM-1), monocyte chemoattractant protein 1 (MCP-1), and neutrophil 
      gelatinase-associated lipocalin (NGAL). Intra- and inter-assay imprecision, 
      linearity under dilution (LUD), spike recovery (S-R), and hemoglobin interference 
      were evaluated using serum from healthy and diseased dogs. Additionally, the 
      effect of sample type (serum vs. plasma) was investigated. All values for Clus 
      and MCP-1 were outside the assay's measurable range. Intra- and inter-assay 
      precision were acceptable for NGAL (CVs 8.8% and 13.2%, respectively). Regression 
      analysis of LUD and S-R indicated good linearity for CysC and NGAL. Hemolysis did 
      not affect measurement of any biomarker. Measured concentrations of CysC (p = 
      0.018) and NGAL (p = 0.015) were significantly lower in sodium citrate plasma 
      compared to serum. We conclude that this magnetic bead-based assay is precise and 
      accurate for NGAL measurement in canine serum. Inappropriate standards for MCP-1 
      and Clus, and poor accuracy for KIM-1 measurement, suggest that this assay cannot 
      reliably quantify those biomarkers in canine blood. Measurements of CysC in 
      canine blood using this assay must be interpreted with caution given inter-assay 
      imprecision.
FAU - Davis, Jennifer
AU  - Davis J
AUID- ORCID: 0000-0002-7078-1645
AD  - College of Veterinary Medicine, School of Science, Health, Engineering, and 
      Education, Murdoch University, Murdoch, Western Australia, Australia.
FAU - Raisis, Anthea L
AU  - Raisis AL
AD  - College of Veterinary Medicine, School of Science, Health, Engineering, and 
      Education, Murdoch University, Murdoch, Western Australia, Australia.
FAU - Miller, David W
AU  - Miller DW
AD  - College of Veterinary Medicine, School of Science, Health, Engineering, and 
      Education, Murdoch University, Murdoch, Western Australia, Australia.
FAU - Rossi, Gabriele
AU  - Rossi G
AUID- ORCID: 0000-0003-4879-9504
AD  - College of Veterinary Medicine, School of Science, Health, Engineering, and 
      Education, Murdoch University, Murdoch, Western Australia, Australia.
LA  - eng
PT  - Journal Article
PT  - Validation Study
DEP - 20200705
PL  - United States
TA  - J Vet Diagn Invest
JT  - Journal of veterinary diagnostic investigation : official publication of the 
      American Association of Veterinary Laboratory Diagnosticians, Inc
JID - 9011490
RN  - 0 (Biomarkers)
SB  - IM
MH  - Acute Kidney Injury/diagnosis/*veterinary
MH  - Animals
MH  - Biomarkers/*blood
MH  - Dog Diseases/*diagnosis
MH  - Dogs
MH  - Female
MH  - Immunoassay/methods/*veterinary
MH  - Male
PMC - PMC7488959
OTO - NOTNLM
OT  - acute kidney injury
OT  - biomarkers
OT  - dogs
OT  - immunoassay
COIS- Declaration of conflicting interests: The authors declared no potential conflicts 
      of interest with respect to the research, authorship and/or publication of this 
      article.
EDAT- 2020/07/07 06:00
MHDA- 2021/01/01 06:00
PMCR- 2021/07/05
CRDT- 2020/07/07 06:00
PHST- 2020/07/07 06:00 [pubmed]
PHST- 2021/01/01 06:00 [medline]
PHST- 2020/07/07 06:00 [entrez]
PHST- 2021/07/05 00:00 [pmc-release]
AID - 10.1177_1040638720939520 [pii]
AID - 10.1177/1040638720939520 [doi]
PST - ppublish
SO  - J Vet Diagn Invest. 2020 Sep;32(5):656-663. doi: 10.1177/1040638720939520. Epub 
      2020 Jul 5.