PMID- 32628833
OWN - NLM
STAT- MEDLINE
DCOM- 20210618
LR  - 20210618
IS  - 1526-4602 (Electronic)
IS  - 1525-7797 (Linking)
VI  - 21
IP  - 8
DP  - 2020 Aug 10
TI  - Monodisperse and Polydisperse PEGylation of Peptides and Proteins: A Comparative 
      Study.
PG  - 3134-3139
LID - 10.1021/acs.biomac.0c00517 [doi]
AB  - Although PEGylation is widely used in biomedicine with great success, it suffers 
      from many drawbacks, such as polydispersity, nonbiodegradability, and loss of 
      precursor potency. Recently, the search for polyethylene glycol (PEG) substitutes 
      has attracted considerable attention. Some of the substitutes partially address 
      the drawbacks of PEGs, but sacrifice the "stealth" effect of PEGs and bring in 
      new issues. Herein, we developed monodisperse oligoethylene glycol (M-OEG) 
      polyamides over 5000 Da as biodegradable and monodisperse PEGylation 
      (M-PEGylation) agents, which provided M-PEGylated peptides and proteins with high 
      monodispersity and a biodegradable PEG moiety. Compared to regular PEGylated 
      proteins with a complex "stealth" cloud of PEG, the hydrogen bond interactions 
      between the M-OEG polyamides and proteins provided the M-PEGylated protein with a 
      biodegradable "stealth" cloak. The monodisperse and biodegradable M-PEGylation 
      strategy as well as the peculiar protein-M-OEG polyamide interactions may shed 
      light on many long-lasting issues during the development of PEGylated biologic 
      drugs, such as monodispersity, biodegradability, and tunable conformation.
FAU - Wang, Jie
AU  - Wang J
AD  - Hubei Province Engineering and Technology Research Center for Fluorinated 
      Pharmaceuticals, School of Pharmaceutical Sciences, Wuhan University, Wuhan 
      430071, P. R. China.
FAU - Deng, Tao
AU  - Deng T
AD  - Hubei Province Engineering and Technology Research Center for Fluorinated 
      Pharmaceuticals, School of Pharmaceutical Sciences, Wuhan University, Wuhan 
      430071, P. R. China.
FAU - Liu, Yuntai
AU  - Liu Y
AD  - Hubei Province Engineering and Technology Research Center for Fluorinated 
      Pharmaceuticals, School of Pharmaceutical Sciences, Wuhan University, Wuhan 
      430071, P. R. China.
FAU - Chen, Kexin
AU  - Chen K
AD  - Hubei Province Engineering and Technology Research Center for Fluorinated 
      Pharmaceuticals, School of Pharmaceutical Sciences, Wuhan University, Wuhan 
      430071, P. R. China.
FAU - Yang, Zhigang
AU  - Yang Z
AUID- ORCID: 0000-0002-4857-4850
AD  - Hubei Province Engineering and Technology Research Center for Fluorinated 
      Pharmaceuticals, School of Pharmaceutical Sciences, Wuhan University, Wuhan 
      430071, P. R. China.
FAU - Jiang, Zhong-Xing
AU  - Jiang ZX
AUID- ORCID: 0000-0003-2601-4366
AD  - Hubei Province Engineering and Technology Research Center for Fluorinated 
      Pharmaceuticals, School of Pharmaceutical Sciences, Wuhan University, Wuhan 
      430071, P. R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200720
PL  - United States
TA  - Biomacromolecules
JT  - Biomacromolecules
JID - 100892849
RN  - 0 (Peptides)
RN  - 0 (Proteins)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
SB  - IM
MH  - *Peptides
MH  - Polyethylene Glycols
MH  - *Proteins
EDAT- 2020/07/07 06:00
MHDA- 2021/06/22 06:00
CRDT- 2020/07/07 06:00
PHST- 2020/07/07 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2020/07/07 06:00 [entrez]
AID - 10.1021/acs.biomac.0c00517 [doi]
PST - ppublish
SO  - Biomacromolecules. 2020 Aug 10;21(8):3134-3139. doi: 10.1021/acs.biomac.0c00517. 
      Epub 2020 Jul 20.