PMID- 32628993
OWN - NLM
STAT- MEDLINE
DCOM- 20210423
LR  - 20210423
IS  - 1879-3177 (Electronic)
IS  - 0887-2333 (Linking)
VI  - 68
DP  - 2020 Oct
TI  - Human keratinocytes and monocytes co-culture cell system: An important 
      contribution for the study of moderate and weak sensitizers.
PG  - 104929
LID - S0887-2333(20)30479-3 [pii]
LID - 10.1016/j.tiv.2020.104929 [doi]
AB  - In vitro approaches to address key events in chemical-induced skin sensitization 
      have been developed, but there is still uncertain how they will be useful to 
      predict the potency for an effective risk assessment. Keratinocytes (KCs) play a 
      key role in all phases of skin sensitization. Dendritic cells (DCs) activation 
      and maturation require the binding of cytokines produced by KC as a result of 
      initial chemical exposure. We previously identified interleukin-18 (IL-18) as 
      useful marker for determination of skin sensitization potential of chemicals. The 
      aim of this paper was to mimic the interaction between KCs and DCs using a 
      co-culture of NCTC 2544 and THP-1 cells. Three selected contact allergens of 
      different potency (Bandrowski's base, diethyl maleate, and imidazolidinyl urea) 
      were tested in time-course experiments (24, 48 and 72 h). Cell surface markers 
      expression (CD80, CD86, and HLA-DR) was determined by flow cytometry analysis 
      while IL-18 production was evaluated with specific sandwich ELISA. Results 
      obtained from this simple in vitro co-culture system show the possibility to 
      study the contribution of KCs in DCs activation through the analysis of HLA-DR 
      expression. Results obtained demonstrate the ability of the KCs to favor the full 
      maturation of the DCs in the presence of moderate and weak allergens, while the 
      extreme allergen induced a complete maturation of DC alone without the need of 
      KCs.
CI  - Copyright (c) 2020 Elsevier Ltd. All rights reserved.
FAU - Galbiati, Valentina
AU  - Galbiati V
AD  - Laboratory of Toxicology, Department of Pharmacological and Biomolecular 
      Sciences, Universita degli Studi di Milano, Via Balzaretti 9, 20133 Milan, Italy. 
      Electronic address: valentina.galbiati@unimi.it.
FAU - Maddalon, Ambra
AU  - Maddalon A
AD  - Laboratory of Toxicology, Department of Pharmacological and Biomolecular 
      Sciences, Universita degli Studi di Milano, Via Balzaretti 9, 20133 Milan, Italy.
FAU - Iulini, Martina
AU  - Iulini M
AD  - Laboratory of Toxicology, Department of Environmental and Political Sciences, 
      Universita degli Studi di Milano, Via Balzaretti 9, 20133 Milan, Italy.
FAU - Marinovich, Marina
AU  - Marinovich M
AD  - Laboratory of Toxicology, Department of Pharmacological and Biomolecular 
      Sciences, Universita degli Studi di Milano, Via Balzaretti 9, 20133 Milan, Italy.
FAU - Corsini, Emanuela
AU  - Corsini E
AD  - Laboratory of Toxicology, Department of Environmental and Political Sciences, 
      Universita degli Studi di Milano, Via Balzaretti 9, 20133 Milan, Italy.
LA  - eng
PT  - Journal Article
DEP - 20200703
PL  - England
TA  - Toxicol In Vitro
JT  - Toxicology in vitro : an international journal published in association with 
      BIBRA
JID - 8712158
RN  - 0 (Allergens)
RN  - 0 (IL18 protein, human)
RN  - 0 (Interleukin-18)
SB  - IM
MH  - Allergens/*toxicity
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Coculture Techniques
MH  - Dendritic Cells/*drug effects/metabolism
MH  - *Dermatitis, Allergic Contact
MH  - Humans
MH  - Interleukin-18/metabolism
MH  - Keratinocytes/*drug effects/metabolism
MH  - Monocytes/drug effects/metabolism
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2020/07/07 06:00
MHDA- 2021/04/24 06:00
CRDT- 2020/07/07 06:00
PHST- 2020/04/03 00:00 [received]
PHST- 2020/06/10 00:00 [revised]
PHST- 2020/06/30 00:00 [accepted]
PHST- 2020/07/07 06:00 [pubmed]
PHST- 2021/04/24 06:00 [medline]
PHST- 2020/07/07 06:00 [entrez]
AID - S0887-2333(20)30479-3 [pii]
AID - 10.1016/j.tiv.2020.104929 [doi]
PST - ppublish
SO  - Toxicol In Vitro. 2020 Oct;68:104929. doi: 10.1016/j.tiv.2020.104929. Epub 2020 
      Jul 3.