PMID- 32630030
OWN - NLM
STAT- MEDLINE
DCOM- 20201123
LR  - 20201123
IS  - 1660-4601 (Electronic)
IS  - 1661-7827 (Print)
IS  - 1660-4601 (Linking)
VI  - 17
IP  - 13
DP  - 2020 Jun 30
TI  - Anti-Inflammatory Potential of Cultured Ginseng Roots Extract in 
      Lipopolysaccharide-Stimulated Mouse Macrophages and Adipocytes.
LID - 10.3390/ijerph17134716 [doi]
LID - 4716
AB  - Wild ginseng, Panax ginseng Meyer, is a traditional medicine widely used in Asia. 
      Due to low reward and high costs, wild ginseng is produced by a plant cell 
      culture technique called cultured ginseng roots (GR). The health benefits of wild 
      ginseng have been well studied, but the potential health effects of GR are 
      largely unknown. Thus, we investigated the role of a GR extract (GRE) on 
      inflammatory responses. We firstly investigated the anti-inflammatory potential 
      of GRE in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. GRE (100 mug/mL) 
      dampened pro-inflammatory gene expression, cytokine release, reactive oxygen 
      species (ROS) production, and mitogen-activated protein kinase (MAPK) activation. 
      These anti-inflammatory responses by GRE were confirmed in mouse bone 
      marrow-derived macrophages (BMDMs), which showed that GRE could inhibit 
      inflammation with the induction of antioxidant levels. LPS was recently reported 
      to impair mitochondrial bioenergetics in mouse macrophages. We next measured the 
      mitochondrial oxygen consumption rate (OCR), determining mitochondrial function. 
      LPS treatment downregulated OCR; however, GRE partially restored the LPS-mediated 
      energy homeostasis defects. Furthermore, GRE-pretreated conditioned media (CM) 
      obtained from mouse macrophages decreased CM-mediated adipocyte inflammation. 
      Collectively, these data suggested that GRE attenuated LPS-induced inflammation, 
      and it might be partially involved in the protection from mitochondrial 
      dysfunction in macrophages and adipocytes.
FAU - Park, Hyun Ju
AU  - Park HJ
AD  - Department of Food Science and Nutrition, Jeju National University, Jeju 63243, 
      Korea.
FAU - Jo, Sang-Mi
AU  - Jo SM
AD  - Department of Food Science and Nutrition, Jeju National University, Jeju 63243, 
      Korea.
FAU - Seo, Seok Hee
AU  - Seo SH
AD  - Department of Food Science and Nutrition, Jeju National University, Jeju 63243, 
      Korea.
FAU - Lee, Myoungsook
AU  - Lee M
AUID- ORCID: 0000-0003-1344-6979
AD  - Department of Food and Nutrition, Sungshin Women's University, Seoul 01133, 
      Korea.
FAU - Lee, Yunkyoung
AU  - Lee Y
AUID- ORCID: 0000-0001-6453-769X
AD  - Department of Food Science and Nutrition, Jeju National University, Jeju 63243, 
      Korea.
FAU - Kang, Inhae
AU  - Kang I
AUID- ORCID: 0000-0001-9352-5591
AD  - Department of Food Science and Nutrition, Jeju National University, Jeju 63243, 
      Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200630
PL  - Switzerland
TA  - Int J Environ Res Public Health
JT  - International journal of environmental research and public health
JID - 101238455
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Cytokines)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Plant Extracts)
SB  - IM
MH  - Adipocytes/drug effects
MH  - Animals
MH  - Anti-Inflammatory Agents/pharmacology
MH  - Asia
MH  - Cytokines
MH  - *Lipopolysaccharides/toxicity
MH  - Macrophages/drug effects
MH  - Mice
MH  - *Panax
MH  - *Plant Extracts/pharmacology
PMC - PMC7369833
OTO - NOTNLM
OT  - adipocytes
OT  - antioxidant
OT  - cultured wild ginseng roots
OT  - inflammation
OT  - macrophages
OT  - mitochondrial dysfunction
OT  - reactive oxygen species
COIS- The authors declare no conflict of interest.
EDAT- 2020/07/08 06:00
MHDA- 2020/11/24 06:00
PMCR- 2020/07/01
CRDT- 2020/07/08 06:00
PHST- 2020/06/17 00:00 [received]
PHST- 2020/06/28 00:00 [revised]
PHST- 2020/06/29 00:00 [accepted]
PHST- 2020/07/08 06:00 [entrez]
PHST- 2020/07/08 06:00 [pubmed]
PHST- 2020/11/24 06:00 [medline]
PHST- 2020/07/01 00:00 [pmc-release]
AID - ijerph17134716 [pii]
AID - ijerph-17-04716 [pii]
AID - 10.3390/ijerph17134716 [doi]
PST - epublish
SO  - Int J Environ Res Public Health. 2020 Jun 30;17(13):4716. doi: 
      10.3390/ijerph17134716.