PMID- 32630236
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 2076-3921 (Print)
IS  - 2076-3921 (Electronic)
IS  - 2076-3921 (Linking)
VI  - 9
IP  - 7
DP  - 2020 Jul 1
TI  - The Impact of Sugar-Sweetened Beverage Consumption on the Liver: A 
      Proteomics-based Analysis.
LID - 10.3390/antiox9070569 [doi]
LID - 569
AB  - Cardiometabolic complications such as the metabolic syndrome and Type 2 Diabetes 
      Mellitus (T2DM) are major causes of global morbidity and mortality. As 
      sugar-sweetened beverages (SSBs) are implicated in this process, this study aimed 
      to obtain greater mechanistic insights. Male Wistar rats (~200 g) were gavaged 
      with a local SSB every day for a period of six months while the control group was 
      gavaged with an iso-volumetric amount of water. Experimental dosages were 
      calculated according to the surface area-to-volume ratio and were equivalent to 
      125 mL/day (in human terms). A proteomic analysis was performed on isolated liver 
      samples and thereafter, markers of endoplasmic reticulum (ER) stress, 
      antioxidant/oxidant capacity, calcium regulation, and mitochondrial functionality 
      were assessed. These data show that SSB consumption resulted in (a) the induction 
      of mild hepatic ER stress; (b) altered hepatic mitochondrial dynamics; and (c) 
      perturbed calcium handling across mitochondria-associated ER membranes. Despite 
      significant changes in markers of ER stress, the antioxidant response and calcium 
      handling (proteomics data), the liver is able to initiate adaptive responses to 
      counteract such stressors. However, the mitochondrial data showed increased 
      fission and decreased fusion that may put the organism at risk for developing 
      insulin resistance and T2DM in the longer term.
FAU - Benade, Janina
AU  - Benade J
AD  - Center for Cardio-Metabolic Research in Africa (CARMA), Department of 
      Physiological Sciences, Stellenbosch University, Stellenbosch 7600, South Africa.
FAU - Sher, Lucien
AU  - Sher L
AD  - Center for Cardio-Metabolic Research in Africa (CARMA), Department of 
      Physiological Sciences, Stellenbosch University, Stellenbosch 7600, South Africa.
FAU - De Klerk, Sheneez
AU  - De Klerk S
AD  - Center for Cardio-Metabolic Research in Africa (CARMA), Department of 
      Physiological Sciences, Stellenbosch University, Stellenbosch 7600, South Africa.
FAU - Deshpande, Gaurang
AU  - Deshpande G
AD  - Center for Cardio-Metabolic Research in Africa (CARMA), Department of 
      Physiological Sciences, Stellenbosch University, Stellenbosch 7600, South Africa.
FAU - Bester, Dirk
AU  - Bester D
AD  - Applied Microbial and Health Biotechnology Institute, Cape Peninsula University 
      of Technology, Bellville 7535, Cape Town, South Africa.
FAU - Marnewick, Jeanine L
AU  - Marnewick JL
AD  - Applied Microbial and Health Biotechnology Institute, Cape Peninsula University 
      of Technology, Bellville 7535, Cape Town, South Africa.
FAU - Sieck, Gary
AU  - Sieck G
AD  - Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN 
      55905, USA.
FAU - Laher, Ismail
AU  - Laher I
AUID- ORCID: 0000-0002-3917-4417
AD  - Center for Cardio-Metabolic Research in Africa (CARMA), Department of 
      Physiological Sciences, Stellenbosch University, Stellenbosch 7600, South Africa.
AD  - Department of Anesthesiology, Pharmacology & Therapeutics, University of British 
      Columbia, Vancouver, BC V6T 1Z3, Canada.
FAU - Essop, M Faadiel
AU  - Essop MF
AUID- ORCID: 0000-0002-8434-4294
AD  - Center for Cardio-Metabolic Research in Africa (CARMA), Department of 
      Physiological Sciences, Stellenbosch University, Stellenbosch 7600, South Africa.
LA  - eng
GR  - IFR150119112480/South African National Research Foundation/
GR  - N/A/Stellenbosch University/
PT  - Journal Article
DEP - 20200701
PL  - Switzerland
TA  - Antioxidants (Basel)
JT  - Antioxidants (Basel, Switzerland)
JID - 101668981
PMC - PMC7402188
OTO - NOTNLM
OT  - antioxidant capacity
OT  - calcium homeostasis
OT  - endoplasmic reticulum stress
OT  - metabolic syndrome
OT  - mitochondrial dysfunction
OT  - mitochondrial fission and fusion
OT  - sugar-sweetened beverages
COIS- The authors declare no conflict of interest.
EDAT- 2020/07/08 06:00
MHDA- 2020/07/08 06:01
PMCR- 2020/07/01
CRDT- 2020/07/08 06:00
PHST- 2020/06/01 00:00 [received]
PHST- 2020/06/15 00:00 [revised]
PHST- 2020/06/19 00:00 [accepted]
PHST- 2020/07/08 06:00 [entrez]
PHST- 2020/07/08 06:00 [pubmed]
PHST- 2020/07/08 06:01 [medline]
PHST- 2020/07/01 00:00 [pmc-release]
AID - antiox9070569 [pii]
AID - antioxidants-09-00569 [pii]
AID - 10.3390/antiox9070569 [doi]
PST - epublish
SO  - Antioxidants (Basel). 2020 Jul 1;9(7):569. doi: 10.3390/antiox9070569.