PMID- 32630646 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20200928 IS - 2076-0817 (Print) IS - 2076-0817 (Electronic) IS - 2076-0817 (Linking) VI - 9 IP - 7 DP - 2020 Jul 2 TI - Core Genome Multilocus Sequence Typing for Food Animal Source Attribution of Human Campylobacter jejuni Infections. LID - 10.3390/pathogens9070532 [doi] LID - 532 AB - Campylobacter jejuni is a major foodborne pathogen and common cause of bacterial enteritis worldwide. A total of 622 C. jejuni isolates recovered from food animals and retail meats in the United States through the National Antimicrobial Resistance Monitoring System between 2013 and 2017 were sequenced using an Illumina MiSeq. Sequences were combined with WGS data of 222 human isolates downloaded from NCBI and analyzed by core genome multilocus sequence typing (cgMLST) and traditional MLST. cgMLST allelic difference (AD) thresholds of 0, 5, 10, 25, 50, 100 and 200 identified 828, 734, 652, 543, 422, 298 and 197 cgMLST types among the 844 isolates, respectively, and traditional MLST identified 174 ST. The cgMLST scheme allowing an AD of 200 (cgMLST(200)) revealed strong correlation with MLST. cgMLST(200) showed 40.5% retail chicken isolates, 56.5% swine, 77.4% dairy cattle and 78.9% beef cattle isolates shared cgMLST sequence type with human isolates. All ST-8 had the same cgMLST(200) type (cgMLST(200)-12) and 74.3% of ST-8 and 75% cgMLST(200)-12 were confirmed as sheep abortion virulence clones by PorA analysis. Twenty-nine acquired resistance genes, including 21 alleles of bla(OXA), tetO, aph(3')-IIIa, ant(6)-Ia, aadE, aad9, aph(2')-Ig, aph(2')-Ih, sat4 plus mutations in gyrA, 23SrRNA and L22 were identified. Resistance genotypes were strongly linked with cgMLST(200) type for certain groups including 12/12 cgMLST(200)-510 with the A103V substitution in L22 and 10/11 cgMLST(200)-608 with the T86I GyrA substitution associated with macrolide and quinolone resistance, respectively. In summary, the cgMLST(200) threshold scheme combined with resistance genotype information could provide an excellent subtyping scheme for source attribution of human C. jejuni infections. FAU - Hsu, Chih-Hao AU - Hsu CH AD - Center for Veterinary Medicine, U.S. Food and Drug Administration, Laurel, MD 20708, USA. FAU - Harrison, Lucas AU - Harrison L AD - Center for Veterinary Medicine, U.S. Food and Drug Administration, Laurel, MD 20708, USA. FAU - Mukherjee, Sampa AU - Mukherjee S AD - Center for Veterinary Medicine, U.S. Food and Drug Administration, Laurel, MD 20708, USA. FAU - Strain, Errol AU - Strain E AD - Center for Veterinary Medicine, U.S. Food and Drug Administration, Laurel, MD 20708, USA. FAU - McDermott, Patrick AU - McDermott P AD - Center for Veterinary Medicine, U.S. Food and Drug Administration, Laurel, MD 20708, USA. FAU - Zhang, Qijing AU - Zhang Q AD - College of Veterinary Medicine, Iowa State University, Ames, IA 50010, USA. FAU - Zhao, Shaohua AU - Zhao S AD - Center for Veterinary Medicine, U.S. Food and Drug Administration, Laurel, MD 20708, USA. LA - eng PT - Journal Article DEP - 20200702 PL - Switzerland TA - Pathogens JT - Pathogens (Basel, Switzerland) JID - 101596317 PMC - PMC7400327 OTO - NOTNLM OT - Campylobacter jejuni OT - PorA OT - cgMLST OT - foodborne pathogens OT - source attribution COIS- The authors declare no conflict of interest. EDAT- 2020/07/08 06:00 MHDA- 2020/07/08 06:01 PMCR- 2020/07/01 CRDT- 2020/07/08 06:00 PHST- 2020/05/19 00:00 [received] PHST- 2020/06/24 00:00 [revised] PHST- 2020/06/29 00:00 [accepted] PHST- 2020/07/08 06:00 [entrez] PHST- 2020/07/08 06:00 [pubmed] PHST- 2020/07/08 06:01 [medline] PHST- 2020/07/01 00:00 [pmc-release] AID - pathogens9070532 [pii] AID - pathogens-09-00532 [pii] AID - 10.3390/pathogens9070532 [doi] PST - epublish SO - Pathogens. 2020 Jul 2;9(7):532. doi: 10.3390/pathogens9070532.